Determining the Full Three-Dimensional Orientation of Single Anisotropic Nanoparticles by Differential Interference Contrast Microscopy

2012 ◽  
Vol 124 (31) ◽  
pp. 7854-7858 ◽  
Author(s):  
Lehui Xiao ◽  
Ji Won Ha ◽  
Lin Wei ◽  
Gufeng Wang ◽  
Ning Fang
Keyword(s):  
Three Dimensional ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Anisotropic Nanoparticles ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

scholarly journals Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis

2003 ◽  
Vol 160 (5) ◽  
pp. 671-683 ◽  
Author(s):  
Alexey Khodjakov ◽  
Lily Copenagle ◽  
Michael B. Gordon ◽  
Duane A. Compton ◽  
Tarun M. Kapoor
Keyword(s):  
Three Dimensional ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Spindle Formation ◽  
Capture Mechanism ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy ◽  
Kinesin Eg5 ◽  
Mitotic Kinesin Eg5 ◽  
Dependent Mechanism

Near-simultaneous three-dimensional fluorescence/differential interference contrast microscopy was used to follow the behavior of microtubules and chromosomes in living α-tubulin/GFP-expressing cells after inhibition of the mitotic kinesin Eg5 with monastrol. Kinetochore fibers (K-fibers) were frequently observed forming in association with chromosomes both during monastrol treatment and after monastrol removal. Surprisingly, these K-fibers were oriented away from, and not directly connected to, centrosomes and incorporated into the spindle by the sliding of their distal ends toward centrosomes via a NuMA-dependent mechanism. Similar preformed K-fibers were also observed during spindle formation in untreated cells. In addition, upon monastrol removal, centrosomes established a transient chromosome-free bipolar array whose orientation specified the axis along which chromosomes segregated. We propose that the capture and incorporation of preformed K-fibers complements the microtubule plus-end capture mechanism and contributes to spindle formation in vertebrates.

Defocused differential interference contrast microscopy imaging of single plasmonic anisotropic nanoparticles

2014 ◽  
Vol 50 (41) ◽  
pp. 5500-5502 ◽  
Author(s):  
Ji Won Ha ◽  
Ning Fang
Keyword(s):  
Spatial Orientation ◽  
Scattered Light ◽  
Gold Nanorod ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Microscopy Imaging ◽  
Anisotropic Nanoparticles ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

The scattered light from a gold nanorod aligned closer to the polarization directions enables us to resolve its spatial orientation under a defocused DIC microscope.

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