scholarly journals Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore

2010 ◽  
Vol 122 (52) ◽  
pp. 10304-10307 ◽  
Author(s):  
John Chu ◽  
Marcos González-López ◽  
Scott L. Cockroft ◽  
Manuel Amorin ◽  
M. Reza Ghadiri
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Real Time Monitoring ◽  
Single Nucleotide ◽  
Dna Strand

scholarly journals Real-Time Monitoring of DNA Polymerase Function and Stepwise Single-Nucleotide DNA Strand Translocation through a Protein Nanopore

2010 ◽  
Vol 49 (52) ◽  
pp. 10106-10109 ◽  
Author(s):  
John Chu ◽  
Marcos González-López ◽  
Scott L. Cockroft ◽  
Manuel Amorin ◽  
M. Reza Ghadiri
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Real Time Monitoring ◽  
Single Nucleotide ◽  
Dna Strand

Real-time monitoring of DNA polymerase activity using molecular beacon

2006 ◽  
Vol 353 (1) ◽  
pp. 141-143 ◽  
Author(s):  
Changbei Ma ◽  
Zhiwen Tang ◽  
Kemin Wang ◽  
Weihong Tan ◽  
Jun Li ◽  
...  
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Molecular Beacon ◽  
Polymerase Activity ◽  
Real Time Monitoring

scholarly journals Real-time single-molecule electronic DNA sequencing by synthesis using polymer-tagged nucleotides on a nanopore array

2016 ◽  
Vol 113 (19) ◽  
pp. 5233-5238 ◽  
Author(s):  
Carl W. Fuller ◽  
Shiv Kumar ◽  
Mintu Porel ◽  
Minchen Chien ◽  
Arek Bibillo ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Real Time ◽  
Lipid Bilayers ◽  
Dna Polymerase ◽  
Single Molecule ◽  
High Throughput Sequencing ◽  
Electrical Current ◽  
Sequencing Data ◽  
Sequencing By Synthesis ◽  
Dna Strand

DNA sequencing by synthesis (SBS) offers a robust platform to decipher nucleic acid sequences. Recently, we reported a single-molecule nanopore-based SBS strategy that accurately distinguishes four bases by electronically detecting and differentiating four different polymer tags attached to the 5′-phosphate of the nucleotides during their incorporation into a growing DNA strand catalyzed by DNA polymerase. Further developing this approach, we report here the use of nucleotides tagged at the terminal phosphate with oligonucleotide-based polymers to perform nanopore SBS on an α-hemolysin nanopore array platform. We designed and synthesized several polymer-tagged nucleotides using tags that produce different electrical current blockade levels and verified they are active substrates for DNA polymerase. A highly processive DNA polymerase was conjugated to the nanopore, and the conjugates were complexed with primer/template DNA and inserted into lipid bilayers over individually addressable electrodes of the nanopore chip. When an incoming complementary-tagged nucleotide forms a tight ternary complex with the primer/template and polymerase, the tag enters the pore, and the current blockade level is measured. The levels displayed by the four nucleotides tagged with four different polymers captured in the nanopore in such ternary complexes were clearly distinguishable and sequence-specific, enabling continuous sequence determination during the polymerase reaction. Thus, real-time single-molecule electronic DNA sequencing data with single-base resolution were obtained. The use of these polymer-tagged nucleotides, combined with polymerase tethering to nanopores and multiplexed nanopore sensors, should lead to new high-throughput sequencing methods.

scholarly journals Real-time monitoring of DNA immobilization and detection of DNA polymerase activity by a microfluidic nanoplasmonic platform

2019 ◽  
Vol 142 ◽  
pp. 111528 ◽  
Author(s):  
Johanna Roether ◽  
Kang-Yu Chu ◽  
Norbert Willenbacher ◽  
Amy Q. Shen ◽  
Nikhil Bhalla
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Polymerase Activity ◽  
Real Time Monitoring ◽  
Dna Immobilization

Label-free molecular beacon for real-time monitoring of DNA polymerase activity

2016 ◽  
Vol 408 (12) ◽  
pp. 3275-3280 ◽  
Author(s):  
Changbei Ma ◽  
Haisheng Liu ◽  
Jun Wang ◽  
Shunxin Jin ◽  
Kemin Wang
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Molecular Beacon ◽  
Polymerase Activity ◽  
Label Free ◽  
Real Time Monitoring

Real-time monitoring of DNA polymerase reactions by a micro ISFET pH sensor

1992 ◽  
Vol 64 (17) ◽  
pp. 1996-1997 ◽  
Author(s):  
Toshinari. Sakurai ◽  
Yuzuru. Husimi
Keyword(s):  
Real Time ◽  
Dna Polymerase ◽  
Ph Sensor ◽  
Real Time Monitoring

1618: Real-Time Monitoring of Nitric Oxide and Blood Flow During Ischemia-Reperfusion in the Rat Testis

2006 ◽  
Vol 175 (4S) ◽  
pp. 521-521
Author(s):  
Motoaki Saito ◽  
Tomoharu Kono ◽  
Yukako Kinoshita ◽  
Itaru Satoh ◽  
Keisuke Satoh
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Real Time ◽  
Ischemia Reperfusion ◽  
Real Time Monitoring ◽  
Rat Testis

GaN heteroepitaxy by remote plasma MOCVD : Real time monitoring by laser reflectance interferometry

2001 ◽  
Vol 11 (PR3) ◽  
pp. Pr3-1175-Pr3-1182 ◽  
Author(s):  
M. Losurdo ◽  
A. Grimaldi ◽  
M. Giangregorio ◽  
P. Capezzuto ◽  
G. Bruno
Keyword(s):  
Real Time ◽  
Real Time Monitoring ◽  
Remote Plasma ◽  
Laser Reflectance
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