Bimodal MR-PET Agent for Quantitative pH Imaging

2010 ◽  
Vol 122 (13) ◽  
pp. 2432-2434 ◽  
Author(s):  
Luca Frullano ◽  
Ciprian Catana ◽  
Thomas Benner ◽  
A. Dean Sherry ◽  
Peter Caravan
Keyword(s):  
Ph Imaging

Quantitative Ratiometric pH Imaging Using a 1,2-Dioxetane Chemiluminescence Resonance Energy Transfer Sensor

2020 ◽  
Author(s):  
Lucas S. Ryan ◽  
Jeni Gerberich ◽  
Uroob Haris ◽  
ralph mason ◽  
Alexander Lippert
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Whole Body ◽  
Photon Flux ◽  
Ph Homeostasis ◽  
Ph Imaging ◽  
Confounding Variables ◽  
Significant Difference

<p>Regulation of physiological pH is integral for proper whole-body and cellular function, and disruptions in pH homeostasis can be both a cause and effect of disease. In light of this, many methods have been developed to monitor pH in cells and animals. In this study, we report a chemiluminescence resonance energy transfer (CRET) probe Ratio-pHCL-1, comprised of an acrylamide 1,2-dioxetane chemiluminescent scaffold with an appended pH-sensitive carbofluorescein fluorophore. The probe provides an accurate measurement of pH between 6.8-8.4, making it viable tool for measuring pH in biological systems. Further, its ratiometric output is independent of confounding variables. Quantification of pH can be accomplished both using common fluorimetry and advanced optical imaging methods. Using an IVIS Spectrum, pH can be quantified through tissue with Ratio-pHCL-1, which has been shown in vitro and precisely calibrated in sacrificed mouse models. Initial studies showed that intraperitoneal injections of Ratio-pHCL-1 into sacrificed mice produce a photon flux of more than 10^10 photons per second, and showed a significant difference in ratio of emission intensities between pH 6.0, 7.0, and 8.0.</p> <b></b><i></i><u></u><sub></sub><sup></sup><br>

Ratiometric MRI Sensors Based on Core-Shell Nanoparticles for Quantitative pH Imaging

2014 ◽  
Vol 26 (19) ◽  
pp. 2989-2992 ◽  
Author(s):  
Satoshi Okada ◽  
Shin Mizukami ◽  
Takao Sakata ◽  
Yutaka Matsumura ◽  
Yoshichika Yoshioka ◽  
...  
Keyword(s):  
Core Shell ◽  
Shell Nanoparticles ◽  
Ph Imaging ◽  
Core Shell Nanoparticles

Application of chemical imaging sensor to in-situ pH imaging in the vicinity of a corroding metal surface

2015 ◽  
Vol 183 ◽  
pp. 137-142 ◽  
Author(s):  
Ko-ichiro Miyamoto ◽  
Sakura Sakakita ◽  
Torsten Wagner ◽  
Michael J. Schöning ◽  
Tatsuo Yoshinobu
Keyword(s):  
Metal Surface ◽  
Chemical Imaging ◽  
Ph Imaging ◽  
Imaging Sensor

scholarly journals In vivo evaluation of tumour acidosis for assessing the early metabolic response and onset of resistance to dichloroacetate by using magnetic resonance pH imaging

2017 ◽  
Vol 51 (2) ◽  
pp. 498-506 ◽  
Author(s):  
Annasofia Anemone ◽  
Lorena Consolino ◽  
Laura Conti ◽  
Francesca Reineri ◽  
Federica Cavallo ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Metabolic Response ◽  
In Vivo Evaluation ◽  
Ph Imaging ◽  
Early Metabolic Response

Application of the pH-Imaging Sensor to Determining the Diffusion Coefficients of Ions in Electrolytic Solutions

2000 ◽  
Vol 39 (Part 2, No. 4A) ◽  
pp. L318-L320 ◽  
Author(s):  
Tatsuo Yoshinobu ◽  
Tetsuro Harada ◽  
Hiroshi Iwasaki
Keyword(s):  
Diffusion Coefficients ◽  
Ph Imaging ◽  
Imaging Sensor

High-resolution optical pH imaging of concrete exposed to chemically corrosive environments

2019 ◽  
Vol 116 ◽  
pp. 231-237 ◽  
Author(s):  
Cyrill Grengg ◽  
Bernhard Müller ◽  
Christoph Staudinger ◽  
Florian Mittermayr ◽  
Johanna Breininger ◽  
...  
Keyword(s):  
High Resolution ◽  
Ph Imaging ◽  
Corrosive Environments

Reversible Self-Assembly of Nanoprobes in Live Cells for Dynamic Intracellular pH Imaging

ACS Nano ◽  
2019 ◽  
Author(s):  
Bo Dong ◽  
Shuangli Du ◽  
Chunxiao Wang ◽  
Haohao Fu ◽  
Qiang Li ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Self Assembly ◽  
Live Cells ◽  
Ph Imaging

Design and validation of a new ratiometric intracellular pH imaging probe using lanthanide-doped upconverting nanoparticles

2017 ◽  
Vol 46 (40) ◽  
pp. 13957-13965 ◽  
Author(s):  
Shuoren Du ◽  
Javier Hernández-Gil ◽  
Hao Dong ◽  
Xiaoyu Zheng ◽  
Guangming Lyu ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Quantitative Imaging ◽  
Living Cells ◽  
Ph Sensitive ◽  
Upconversion Nanoparticles ◽  
Imaging Probe ◽  
Ph Imaging ◽  
Ratiometric Probe ◽  
Subcellular Level

A ratiometric probe based on upconversion nanoparticles modified with a pH sensitive moiety for the quantitative imaging of pH at the subcellular level in living cells.

Synthesis and tissue distribution of four Se-labeled tertiary amines, potential brain pH imaging agents

1990 ◽  
Vol 17 (6) ◽  
pp. 601-607
Author(s):  
Alain Plenevaux ◽  
Robert Cantineau ◽  
Claude Brihaye ◽  
Christian Lemaire ◽  
Léon Christiaens ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Imaging Agents ◽  
Tertiary Amines ◽  
Ph Imaging ◽  
Brain Ph

scholarly journals High-Resolution pH Imaging of Living Bacterial Cells To Detect Local pH Differences

mBio ◽  
2016 ◽  
Vol 7 (6) ◽  
Author(s):  
Yusuke V. Morimoto ◽  
Nobunori Kami-ike ◽  
Tomoko Miyata ◽  
Akihiro Kawamoto ◽  
Takayuki Kato ◽  
...  
Keyword(s):  
High Resolution ◽  
Atp Hydrolysis ◽  
Imaging System ◽  
Proton Motive Force ◽  
Energy Transduction ◽  
Protein Export ◽  
Motive Force ◽  
Bacterial Flagellum ◽  
Ph Imaging ◽  
Local Ph

ABSTRACTProtons are utilized for various biological activities such as energy transduction and cell signaling. For construction of the bacterial flagellum, a type III export apparatus utilizes ATP and proton motive force to drive flagellar protein export, but the energy transduction mechanism remains unclear. Here, we have developed a high-resolution pH imaging system to measure local pH differences within livingSalmonella entericacells, especially in close proximity to the cytoplasmic membrane and the export apparatus. The local pH near the membrane was ca. 0.2 pH unit higher than the bulk cytoplasmic pH. However, the local pH near the export apparatus was ca. 0.1 pH unit lower than that near the membrane. This drop of local pH depended on the activities of both transmembrane export components and FliI ATPase. We propose that the export apparatus acts as an H+/protein antiporter to couple ATP hydrolysis with H+flow to drive protein export.IMPORTANCEThe flagellar type III export apparatus is required for construction of the bacterial flagellum beyond the cellular membranes. The export apparatus consists of a transmembrane export gate and a cytoplasmic ATPase complex. The export apparatus utilizes ATP and proton motive force as the energy source for efficient and rapid protein export during flagellar assembly, but it remains unknown how. In this study, we have developed anin vivopH imaging system with high spatial and pH resolutions with a pH indicator probe to measure local pH near the export apparatus. We provide direct evidence suggesting that ATP hydrolysis by the ATPase complex and the following rapid protein translocation by the export gate are both linked to efficient proton translocation through the gate.

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