Titelbild: In Situ Assembly and Screening of Enzyme Inhibitors with Surface-Tension Microarrays (Angew. Chem. 41/2009)

2009 ◽  
Vol 121 (41) ◽  
pp. 7589-7589
Author(s):  
Laurent Mugherli ◽  
Olga N. Burchak ◽  
Larissa A. Balakireva ◽  
Aline Thomas ◽  
François Chatelain ◽  
...  
Keyword(s):  
Surface Tension ◽  
Enzyme Inhibitors

In Situ Assembly and Screening of Enzyme Inhibitors with Surface-Tension Microarrays

2009 ◽  
Vol 121 (41) ◽  
pp. 7775-7780 ◽  
Author(s):  
Laurent Mugherli ◽  
Olga N. Burchak ◽  
Larissa A. Balakireva ◽  
Aline Thomas ◽  
François Chatelain ◽  
...  
Keyword(s):  
Surface Tension ◽  
Enzyme Inhibitors

Cover Picture: In Situ Assembly and Screening of Enzyme Inhibitors with Surface-Tension Microarrays (Angew. Chem. Int. Ed. 41/2009)

2009 ◽  
Vol 48 (41) ◽  
pp. 7453-7453 ◽  
Author(s):  
Laurent Mugherli ◽  
Olga N. Burchak ◽  
Larissa A. Balakireva ◽  
Aline Thomas ◽  
François Chatelain ◽  
...  
Keyword(s):  
Surface Tension ◽  
Enzyme Inhibitors

Titelbild: In Situ Assembly and Screening of Enzyme Inhibitors with Surface-Tension Microarrays (Angew. Chem. 41/2009)

2009 ◽  
Vol 121 (41) ◽  
pp. 7589-7589
Author(s):  
Laurent Mugherli ◽  
Olga N. Burchak ◽  
Larissa A. Balakireva ◽  
Aline Thomas ◽  
François Chatelain ◽  
...  
Keyword(s):  
Surface Tension ◽  
Enzyme Inhibitors

Cover Picture: In Situ Assembly and Screening of Enzyme Inhibitors with Surface-Tension Microarrays (Angew. Chem. Int. Ed. 41/2009)

2009 ◽  
Vol 48 (41) ◽  
pp. 7453-7453
Author(s):  
Laurent Mugherli ◽  
Olga N. Burchak ◽  
Larissa A. Balakireva ◽  
Aline Thomas ◽  
François Chatelain ◽  
...  
Keyword(s):  
Surface Tension ◽  
Enzyme Inhibitors

In Situ Assembly and Screening of Enzyme Inhibitors with Surface-Tension Microarrays

2009 ◽  
Vol 48 (41) ◽  
pp. 7639-7644 ◽  
Author(s):  
Laurent Mugherli ◽  
Olga N. Burchak ◽  
Larissa A. Balakireva ◽  
Aline Thomas ◽  
François Chatelain ◽  
...  
Keyword(s):  
Surface Tension ◽  
Enzyme Inhibitors

scholarly journals Network of Palladium-Based Nanorings Synthesized by Liquid-Phase Reduction Using DMSO-H2O: In Situ Monitoring of Structure Formation and Drying Deformation by ASEM

2020 ◽  
Vol 21 (9) ◽  
pp. 3271 ◽  
Author(s):  
Takuki Komenami ◽  
Akihiro Yoshimura ◽  
Yasunari Matsuno ◽  
Mari Sato ◽  
Chikara Sato
Keyword(s):  
Surface Tension ◽  
Liquid Phase ◽  
Aqueous Solutions ◽  
Critical Point ◽  
Synthesis Method ◽  
Three Dimensional ◽  
In Situ Monitoring ◽  
Critical Point Drying ◽  
Micrometer Size

We developed a liquid-phase synthesis method for Pd-based nanostructure, in which Pd dissolved in dimethyl sulfoxide (DMSO) solutions was precipitated using acid aqueous solution. In the development of the method, in situ monitoring using atmospheric scanning electron microscopy (ASEM) revealed that three-dimensional (3D) Pd-based nanonetworks were deformed to micrometer-size particles possibly by the surface tension of the solutions during the drying process. To avoid surface tension, critical point drying was employed to dry the Pd-based precipitates. By combining ASEM monitoring with critical point drying, the synthesis parameters were optimized, resulting in the formation of lacelike delicate nanonetworks using citric acid aqueous solutions. Precipitation using HCl acid aqueous solutions allowed formation of 500-nm diameter nanorings connected by nanowires. The 3D nanostructure formation was controllable and modifiable into various shapes using different concentrations of the Pd and Cl ions as the parameters.

scholarly journals Development and Validation of a Simple Cell-Based Fluorescence Assay for Dipeptidyl Peptidase 1 (DPP1) Activity

2010 ◽  
Vol 16 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Bob Thong ◽  
James Pilling ◽  
Edward Ainscow ◽  
Raj Beri ◽  
John Unitt
Keyword(s):  
Cell Sorting ◽  
Enzyme Inhibitors ◽  
Dipeptidyl Peptidase ◽  
Fluorescence Assay ◽  
Enzyme Assays ◽  
Fluorogenic Substrates ◽  
Intracellular Degradation ◽  
Dipeptidyl Aminopeptidase ◽  
Development And Validation

Dipeptidyl peptidase 1 (DPP1) (EC 3.4.14.1; also known as cathepsin C, cathepsin J, dipeptidyl aminopeptidase, and dipeptidyl aminotransferase) is a lysosomal cysteinyl protease of the papain family involved in the intracellular degradation of proteins. Isolated enzyme assays for DPP1 activity using a variety of synthetic substrates such as dipeptide or peptide linked to amino-methyl-coumarin (AMC) or other fluorophores are well established. There is, however, no report of a simple whole-cell-based assay for measuring lysosomal DPP1 activity other than the use of flow cytometry (fluorescence-activated cell sorting) or the use of invasive activity-based probes or the production of physiological products such as neutrophil elastase. The authors investigated a number of DPP1 fluorogenic substrates that have the potential to access the lysosome and enable the measurement of DPP1 enzyme activity in situ. They describe the development and evaluation of a simple noninvasive fluorescence assay for measuring DPP1 activity in fresh or cryopreserved human THP-1 cells using the substrate H-Gly-Phe-AFC (amino-fluoro-coumarin). This cell-based fluorescence assay can be performed in a 96-well plate format and is ideally suited for determining the cell potency of potential DPP1 enzyme inhibitors.

In situ assembly of enzyme inhibitors using extended tethering

2003 ◽  
Vol 21 (3) ◽  
pp. 308-314 ◽  
Author(s):  
Daniel A. Erlanson ◽  
Joni W. Lam ◽  
Christian Wiesmann ◽  
Tinh N. Luong ◽  
Robert L. Simmons ◽  
...  
Keyword(s):  
Enzyme Inhibitors

Investigation of Surface Tension Effects of a Water-Hexadecane Interface on Silicon Microparts

2011 ◽  
Author(s):  
Caroline E. Liberti ◽  
Nathan B. Crane
Keyword(s):  
Surface Tension ◽  
Low Cost ◽  
Photovoltaic Cells ◽  
In Situ Fabrication ◽  
Recent Interest

Assembly at sub-millimeter dimensions is a challenging process that is often not economically feasible. This limits many systems to in-situ fabrication from compatible materials. If freed from these limitations on processes and materials, it might be possible to improve microsystem performance. One critical application of recent interest is in the assembly of small crystalline photovoltaic cells onto low-cost and possibly flexible modules [1].

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