A Practical Solid-Phase Synthesis of Glu7-Phalloidin and Entry into Fluorescent F-Actin-Binding Reagents

2007 ◽  
Vol 119 (19) ◽  
pp. 3617-3619 ◽  
Author(s):  
Laura A. Schuresko ◽  
R. Scott Lokey
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Actin Binding ◽  
Phase Synthesis

Solid-phase synthesis of a novel phalloidin analog with on-bead and off-bead actin-binding activity

2019 ◽  
Vol 55 (3) ◽  
pp. 385-388 ◽  
Author(s):  
Antoine Blanc ◽  
Mihajlo Todorovic ◽  
David M. Perrin
Keyword(s):  
Cellular Response ◽  
Actin Polymerization ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Binding Activity ◽  
Cellular Morphology ◽  
Actin Binding ◽  
Phase Synthesis

Specific effectors of actin polymerization have found use as dynamic probes of cellular morphology that may be used to gauge cellular response to stimuli and drugs.

Synthesis and Screening of A DNA-Encoded Library of Non-Peptidic Macrocycles

2020 ◽  
Author(s):  
Eric Koesema ◽  
Animesh Roy ◽  
Nicholas G. Paciaroni ◽  
Thomas Kodadek
Keyword(s):  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Cell Permeability ◽  
Phase Synthesis ◽  
Protein Ligands ◽  
High Affinity ◽  
Scaffold Diversity

There is considerable interest in the development of libraries of non-peptidic macrocycles as a source of ligands for difficult targets. We report here the solid-phase synthesis of a DNA-encoded library of several hundred thousand thioether-linked macrocycles. The library was designed to be highly diverse with respect to backbone scaffold diversity and to minimize the number of amide N-H bonds, which compromise cell permeability. The utility of the library as a source of protein ligands is demonstrated through the isolation of compounds that bind streptavidin, a model target, with high affinity.

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