scholarly journals Refining murine heterotopic heart transplantation: A model to study ischemia and reperfusion injury in donation after circulatory death hearts

2021 ◽  
Author(s):  
Mohammed Quader ◽  
Renee Cholyway ◽  
Niluka Wickramaratne ◽  
Oluwatoyin Akande ◽  
Martin Mangino ◽  
...  
Keyword(s):  
Heart Transplantation ◽  
Reperfusion Injury ◽  
Ischemia And Reperfusion ◽  
Donation After Circulatory Death ◽  
Ischemia And Reperfusion Injury ◽  
Heterotopic Heart Transplantation ◽  
Circulatory Death

scholarly journals Ischemia and reperfusion injury to mitochondria and cardiac function in donation after circulatory death hearts- an experimental study

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243504
Author(s):  
Oluwatoyin Akande ◽  
Qun Chen ◽  
Stefano Toldo ◽  
Edward J. Lesnefsky ◽  
Mohammed Quader
Keyword(s):  
Cardiac Function ◽  
Heart Transplantation ◽  
Ischemia And Reperfusion ◽  
Donation After Circulatory Death ◽  
Ischemia And Reperfusion Injury ◽  
Donor Pool ◽  
Retention Capacity ◽  
Donation Process ◽  
Heart Donor ◽  
Circulatory Death

The ultimate treatment for patients with end-stage heart failure is heart transplantation. The number of donor hearts which are primarily procured from donation after brain death (DBD) donors is limited, but donation after circulatory death (DCD) donor hearts can increase the heart donor pool. However, ischemia and reperfusion injuries associated with the DCD process causes myocardial damage, limiting the use of DCD hearts in transplantation. Addressing this problem is critical in the exploration of DCD hearts as suitable donor hearts for transplantation. In this study, rat hearts were procured following the control beating-heart donor (CBD) or DCD donation process. Changes in mitochondria and cardiac function from DCD hearts subjected to 25 or 35 minutes of ischemia followed by 60 minutes of reperfusion were compared to CBD hearts. Following ischemia, rates of oxidative phosphorylation and calcium retention capacity were progressively impaired in DCD hearts compared to CBD hearts. Reperfusion caused additional mitochondrial dysfunction in DCD hearts. Developed pressure, inotropy and lusitropy, were significantly reduced in DCD hearts compared to CBD hearts. We, therefore, suggest that interventional strategies targeted before the onset of ischemia and at reperfusion could protect mitochondria, thus potentially making DCD hearts suitable for heart transplantation.

Splanchnic ischemia and reperfusion injury is reduced by genetic or pharmacological inhibition of TNF-α

2007 ◽  
Vol 81 (4) ◽  
pp. 1032-1043 ◽  
Author(s):  
Emanuela Esposito ◽  
Emanuela Mazzon ◽  
Carmelo Muià ◽  
Rosaria Meli ◽  
Edoardo Sessa ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia And Reperfusion ◽  
Ischemia And Reperfusion Injury ◽  
Pharmacological Inhibition ◽  
Tnf Α ◽  
Splanchnic Ischemia
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