Systemic prednisone administration selectively alters granulocyte subsets in nasal polyps from aspirin-exacerbated respiratory disease and chronic rhinosinusitis patients

2013 ◽  
Vol 3 (11) ◽  
pp. 866-876 ◽  
Author(s):  
Justin A. Edward ◽  
Mrinmoy Sanyal ◽  
Vijay R. Ramakrishnan ◽  
Wei Le ◽  
Alan L. Nguyen ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Respiratory Disease ◽  
Nasal Polyps ◽  
Aspirin Exacerbated Respiratory Disease

scholarly journals IL-5Rα marks nasal polyp IgG4 and IgE-secreting cells in aspirin-exacerbated respiratory disease

2019 ◽  
Author(s):  
Kathleen M. Buchheit ◽  
Daniel F. Dwyer ◽  
Jose Ordovas-Montanes ◽  
Howard R. Katz ◽  
Erin Lewis ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Respiratory Disease ◽  
Disease Severity ◽  
Nasal Polyp ◽  
Nasal Polyps ◽  
Mrna Levels ◽  
Aspirin Exacerbated Respiratory Disease ◽  
Polyp Tissue ◽  
Antibody Levels ◽  
Antibody Secreting Cells

AbstractBackgroundThe cause of nasal polyposis in aspirin-exacerbated respiratory disease (AERD) is unknown. Elevated antibody levels have been associated with disease severity in nasal polyps, but the upstream drivers and cellular mechanisms of local antibody production in AERD remain to be investigated.ObjectiveWe sought to identify the upstream drivers and phenotypic properties of local antibody-secreting cells in nasal polyps and to understand their clinical relevance in AERD.MethodsSinus tissue was obtained from subjects with AERD, aspirin-tolerant chronic rhinosinusitis with nasal polyps (CRSwNP), aspirin-tolerant chronic rhinosinusitis without nasal polyps (CRSsNP), and healthy controls. Tissue antibody levels were quantified via ELISA and immunohistochemistry, and were correlated with clinical markers of disease severity. Tissue cytokine mRNA levels were measured with quantitative PCR (qPCR). Antibody-secreting cells were profiled with a combination of single-cell RNA-sequencing (scRNA-seq), flow cytometry and immunofluorescence.ResultsTissue IgE and IgG4 were elevated in AERD compared to controls (p<0.01 for IgE and p<0.001 for IgG4, vs. CRSwNP). Total IgG and IgG4 positively correlated with the number of polyp surgeries per subject (r=0.48, p=0.011 and r=0.58, p=0.0003, respectively). Polyp IL-10 mRNA expression was higher in AERD vs. CRSwNP (p<0.05), but there were no differences in mRNA expression of type 2 cytokines. ScRNA-seq revealed increased IL5RA, IGHG4, and IGHE in the antibody-associated cells of subjects with AERD compared to CRSwNP. Total plasma cells and IL-5Rα+ plasma cell numbers in the polyp tissue from AERD exceeded those in polyps from CRSwNP (p=0.0051 and p=0.026, respectively) by flow cytometry. With immunofluorescence, we determined that IL-5Rα and IgG4 are co-expressed in antibody-secreting cells in AERD.ConclusionsOur study identifies unique clusters of antibody-secreting cells in AERD defined by enrichment of transcripts encoding IL5RA, IGHG4 and IGHE. We confirm surface expression of IL-5Rα on these cells, and identify T cells as a unique transcriptional source of IL-5. Tissue antibody levels are elevated in AERD and correlate with disease severity. Our findings suggest a role for IL-5 in facilitating local antibody production that may drive features of severe sinus disease.Key MessagesIgG4 and IgE levels are markedly increased in nasal polyp tissue from subjects with AERD compared to aspirin-tolerant CRSwNP.Tissue IgG4 levels positively correlate with disease recurrence.IL-10 mRNA levels are significantly higher in AERD polyp tissue compared to CRSwNP tissue, but differences were not noted for type 2 cytokines or cytokines involved in class switch recombination.IL-5Rα transcript and protein surface expression is elevated in antibody-secreting cells from subjects with AERD and may play a role in facilitating class switching and/or survival of antibody-secreting cells.Capsule SummarySingle-cell RNA-sequencing (scRNA-seq) of whole nasal polyp tissue identified increased IL5RA, IGHE, and IGHG4 expression in the antibody-secreting cell compartment of subjects with aspirin-exacerbated respiratory disease (AERD) compared to aspirin-tolerant chronic rhinosinusitis with nasal polyps (CRSwNP). IgE and IgG4 levels are elevated in nasal polyp tissue from subjects with AERD compared to CRSwNP and correlate with disease recurrence.

Systemic expression of inflammatory mediators in patients with chronic rhinosinusitis and nasal polyps with and without Aspirin Exacerbated Respiratory Disease

Cytokine ◽  
2016 ◽  
Vol 77 ◽  
pp. 157-167 ◽  
Author(s):  
Rogerio Pezato ◽  
Monika Świerczyńska-Krępa ◽  
Ewa Niżankowska-Mogilnicka ◽  
Gabriele Holtappels ◽  
Natalie De Ruyck ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Respiratory Disease ◽  
Inflammatory Mediators ◽  
Nasal Polyps ◽  
Aspirin Exacerbated Respiratory Disease

scholarly journals A genetic variant near TSLP is associated with chronic rhinosinusitis with nasal polyps and aspirin-exacerbated respiratory disease in Japanese populations

2020 ◽  
Vol 69 (1) ◽  
pp. 138-140 ◽  
Author(s):  
Tsuguhisa Nakayama ◽  
Tomomitsu Hirota ◽  
Daiya Asaka ◽  
Masafumi Sakashita ◽  
Takahiro Ninomiya ◽  
...  
Keyword(s):  
Chronic Rhinosinusitis ◽  
Respiratory Disease ◽  
Nasal Polyps ◽  
Genetic Variant ◽  
Aspirin Exacerbated Respiratory Disease

scholarly journals Associations between the Quality of Life and Nasal Polyp Size in Patients Suffering from Chronic Rhinosinusitis without Nasal Polyps, with Nasal Polyps or Aspirin-Exacerbated Respiratory Disease

2020 ◽  
Vol 9 (4) ◽  
pp. 925 ◽  
Author(s):  
Sven Schneider ◽  
Nicholas J. Campion ◽  
Sergio Villazala-Merino ◽  
David Tianxiang Liu ◽  
Tina Bartosik ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Chronic Rhinosinusitis ◽  
Respiratory Disease ◽  
Nasal Polyp ◽  
Nasal Polyps ◽  
Common Disease ◽  
Polyp Size ◽  
Aspirin Exacerbated Respiratory Disease ◽  
The Impact

Chronic rhinosinusitis (CRS) is a common disease that substantially impairs the quality of life (QoL). Here, we aimed to assess patients’ QoL in different subtypes of CRS and correlated this with nasal polyp size to improve the clinical understanding of the burden of disease. In this retrospective single-center study, 107 patients with the following diagnoses were analyzed: CRS without nasal polyps (CRSsNP), CRS with nasal polyps (CRSwNP), or aspirin-exacerbated respiratory disease (AERD). Sino-Nasal Outcome Test-20 German Adapted Version (SNOT-20 GAV) scores and their correlation with endoscopic Total Polyp Scores (TPS) were evaluated. The mean SNOT-20 GAV scores were highest in patients with AERD (AERD = 43.4, CRSwNP = 36.3, CRSsNP = 30.9). A statistically significant correlation of total SNOT-20 GAV score with TPS was observed in CRSwNP patients (r = 0.3398, p = 0.0195), but not in AERD patients (r = 0.2341, p = 0.1407). When analyzing single SNOT-20 parameters, a strong correlation with TPS was observed for blockage/congestion of the nose, particularly in AERD patients (r = 0.65, p < 0.0001). The impact of nasal polyp size on the QoL differs amongst the subgroups of CRS. Nasal symptoms have the greatest impact on QoL in patients suffering from AERD. CRSwNP and AERD patients should be separately analyzed in clinical investigations and interpretations due to significant differences in QoL.

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