scholarly journals Whole genome microarray analysis of gene expression in an imprinting center deletion mouse model of Prader–Willi syndrome

2007 ◽  
Vol 143A (5) ◽  
pp. 422-429 ◽  
Author(s):  
Douglas C. Bittel ◽  
Nataliya Kibiryeva ◽  
Steven G. McNulty ◽  
Daniel J. Driscoll ◽  
Merlin G. Butler ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mouse Model ◽  
Microarray Analysis ◽  
Prader Willi Syndrome ◽  
Whole Genome ◽  
Imprinting Center ◽  
Whole Genome Microarray

scholarly journals Whole genome microarray analysis of gene expression in Prader–Willi syndrome

2007 ◽  
Vol 143A (5) ◽  
pp. 430-442 ◽  
Author(s):  
Douglas C. Bittel ◽  
Nataliya Kibiryeva ◽  
Susan M. Sell ◽  
Theresa V. Strong ◽  
Merlin G. Butler
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Prader Willi Syndrome ◽  
Whole Genome ◽  
Whole Genome Microarray

scholarly journals The absence of P2X7 receptors (P2rx7) on non-haematopoietic cells leads to selective alteration in mood-related behaviour with dysregulated gene expression and stress reactivity in mice

2013 ◽  
Vol 16 (1) ◽  
pp. 213-233 ◽  
Author(s):  
Cecilia Csölle ◽  
Rómeó D. Andó ◽  
Ágnes Kittel ◽  
Flóra Gölöncsér ◽  
Mária Baranyi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Stress Reactivity ◽  
Therapeutic Potential ◽  
Forced Swim Test ◽  
Synaptic Function ◽  
Whole Genome ◽  
Haematopoietic Cells ◽  
Whole Genome Microarray ◽  
Genetic Deletion

Abstract The purpose of this study was to explore how genetic deletion and pharmacological antagonism of the P2X7 receptor (P2rx7) alter mood-related behaviour, gene expression and stress reactivity in the brain. The forced swim test (FST), tail suspension test (TST) and amphetamine-induced hyperlocomotion (AH) tests were used in wild-type (P2rx7+/+) and P2rx7-deficient (P2rx7−/−) mice. Biogenic amine levels were analysed in the amygdala and striatum, adrenocorticotropic hormone (ACTH) and corticosterone levels were measured in the plasma and pituitary after restraint stress. Chimeric mice were generated by bone marrow transplantation. A whole genome microarray analysis with real-time polymerase chain reaction validation was performed on the amygdala. In the absence of P2rx7s decreased behavioural despair in the FST, reduced immobility in the TST and attenuated amphetamine-induced hyperactivity were detected. Basal norepinephrine levels were elevated in the amygdala, whereas stress-induced ACTH and corticosterone responses were alleviated in P2rx7−/− mice. Sub-acute treatment with the selective P2rx7 antagonist, Brilliant Blue G, reproduced the effect of genetic deletion in the TST and AH test in P2rx7+/+ but not P2rx7−/− mice. No change in behavioural phenotype was observed in chimeras lacking the P2rx7 in their haematopoietic compartment. Whole genome microarray analysis indicated a widespread up- and down-regulation of genes crucial for synaptic function and neuroplasticity by genetic deletion. Here, we present evidence that the absence of P2rx7s on non-haematopoietic cells leads to a mood-stabilizing phenotype in several behavioural models and suggest a therapeutic potential of P2rx7 antagonists for the treatment of mood disorders.

scholarly journals Whole genome microarray analysis ofC. elegans rrf-3anderi-1mutants

FEBS Letters ◽  
2007 ◽  
Vol 581 (26) ◽  
pp. 5050-5054 ◽  
Author(s):  
Suvi Asikainen ◽  
Markus Storvik ◽  
Merja Lakso ◽  
Garry Wong
Keyword(s):  
Microarray Analysis ◽  
Whole Genome ◽  
Whole Genome Microarray
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