scholarly journals A novel pooled-sample multiplex luminex assay for high-throughput measurement of relative telomere length

2018 ◽  
Vol 30 (4) ◽  
pp. e23118 ◽  
Author(s):  
Farzana Jasmine ◽  
Justin Shinkle ◽  
Mekala Sabarinathan ◽  
Habibul Ahsan ◽  
Brandon L. Pierce ◽  
...  
Keyword(s):  
Telomere Length ◽  
High Throughput ◽  
Luminex Assay ◽  
Relative Telomere Length ◽  
Pooled Sample

scholarly journals Glucocorticoids, state-dependent reproductive investment and success in the face of danger in a long-lived bird

2020 ◽  
Author(s):  
Kristina Noreikienė ◽  
Kim Jaatinen ◽  
Benjamin B. Steele ◽  
Markus Öst
Keyword(s):  
Predation Risk ◽  
Telomere Length ◽  
Clutch Size ◽  
Body Condition ◽  
Nest Predation ◽  
Hatching Success ◽  
Reproductive Investment ◽  
Trade Off ◽  
Relative Telomere Length ◽  
Future Reproduction

AbstractGlucocorticoid hormones may mediate trade-offs between current and future reproduction. However, understanding their role is complicated by predation risk, which simultaneously affects the value of the current reproductive investment and elevates glucocorticoid levels. Here, we shed light on these issues in long-lived female Eiders (Somateria mollissima) by investigating how current reproductive investment (clutch size) and hatching success relate to faecal glucocorticoid metabolite [fGCM] level and residual reproductive value (minimum years of breeding experience, body condition, relative telomere length) under spatially variable predation risk. Our results showed a positive relationship between colony-specific predation risk and mean colony-specific fGCM levels. Clutch size and female fGCM were negatively correlated only under high nest predation and in females in good body condition, previously shown to have a longer life expectancy. We also found that younger females with longer telomeres had smaller clutches. The drop in hatching success with increasing fGCM levels was least pronounced under high nest predation risk, suggesting that elevated fGCM levels may allow females to ensure some reproductive success under such conditions. Hatching success was positively associated with female body condition, with relative telomere length, particularly in younger females, and with female minimum age, particularly under low predation risk, showing the utility of these metrics as indicators of individual quality. In line with a trade-off between current and future reproduction, our results show that high potential for future breeding prospects and increased predation risk shift the balance toward investment in future reproduction, with glucocorticoids playing a role in the resolution of this trade-off.

Correlation between metabolic syndrome and relative telomere length shortening in HIV/AIDS patients on combined antiretroviral therapy

2021 ◽  
Vol 147 ◽  
pp. 111269
Author(s):  
Gordana Dragović ◽  
Mladen Andjić ◽  
Boško Toljić ◽  
Djordje Jevtović ◽  
Relja Lukić ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Antiretroviral Therapy ◽  
Telomere Length ◽  
Aids Patients ◽  
Relative Telomere Length ◽  
Combined Antiretroviral Therapy ◽  
Hiv Aids

scholarly journals Urinary arsenic and relative telomere length in 5–7 year old children in Bangladesh

2021 ◽  
Vol 156 ◽  
pp. 106765
Author(s):  
Shohreh F. Farzan ◽  
Mohammad Shahriar ◽  
Muhammad G. Kibriya ◽  
Farzana Jasmine ◽  
Golam Sarwar ◽  
...  
Keyword(s):  
Telomere Length ◽  
Urinary Arsenic ◽  
Relative Telomere Length

Relative telomere length is associated with mortality in prostate cancer patients

2019 ◽  
Vol 18 (11) ◽  
pp. e3488
Author(s):  
T. Langsenlehner ◽  
K. Lukasiak ◽  
H-J. Gruber ◽  
M. Hermann ◽  
U. Langsenlehner ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Telomere Length ◽  
Cancer Patients ◽  
Relative Telomere Length

Relative telomere length and gene expression of shelterin complex proteins among vinyl chloride monomer‐exposed workers in China

2019 ◽  
Vol 60 (4) ◽  
pp. 361-367 ◽  
Author(s):  
Guo‐Qiao Zheng ◽  
Guang‐Hui Zhang ◽  
Han‐Tian Wu ◽  
Yu‐Ting Tu ◽  
Wei Tian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Telomere Length ◽  
Vinyl Chloride ◽  
Vinyl Chloride Monomer ◽  
Shelterin Complex ◽  
Relative Telomere Length ◽  
Exposed Workers

scholarly journals Massively parallel single-molecule telomere length measurement with digital real-time PCR

2020 ◽  
Vol 6 (34) ◽  
pp. eabb7944 ◽  
Author(s):  
Yongqiang Luo ◽  
Ramya Viswanathan ◽  
Manoor Prakash Hande ◽  
Amos Hong Pheng Loh ◽  
Lih Feng Cheow
Keyword(s):  
Telomere Length ◽  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Real Time Pcr ◽  
Length Distribution ◽  
Telomere Maintenance ◽  
Massively Parallel ◽  
Primary Tumors ◽  
Absolute Length

Telomere length is a promising biomarker for age-associated diseases and cancer, but there are still substantial challenges to routine telomere analysis in clinics because of the lack of a simple and rapid yet scalable method for measurement. We developed the single telomere absolute-length rapid (STAR) assay, a novel high-throughput digital real-time PCR approach for rapidly measuring the absolute lengths and quantities of individual telomere molecules. We show that this technique provides the accuracy and sensitivity to uncover associations between telomere length distribution and telomere maintenance mechanisms in cancer cell lines and primary tumors. The results indicate that the STAR assay is a powerful tool to enable the use of telomere length distribution as a biomarker in disease and population-wide studies.

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