scholarly journals Identification of Flt3 internal tandem duplications downstream targets by high-throughput immunoblotting protein array system

2006 ◽  
Vol 81 (9) ◽  
pp. 717-719 ◽  
Author(s):  
Shinichiro Takahashi
Keyword(s):  
High Throughput ◽  
Protein Array ◽  
Downstream Targets ◽  
Tandem Duplications

DNA-LINKED PROTEIN ARRAY FOR HIGH-THROUGHPUT PROTEOMICS: FROM SPATIALLY UNKNOWN DNA ARRAYS TO IDENTIFIABLE PROTEIN ARRAYS

Nano LIFE ◽  
2010 ◽  
Vol 01 (01n02) ◽  
pp. 33-43 ◽  
Author(s):  
MANISH BIYANI ◽  
NAOTO NEMOTO ◽  
TAKANORI ICHIKI
Keyword(s):  
High Throughput ◽  
Protein Array ◽  
Functional Proteomics ◽  
Functional Assay ◽  
Dna Arrays ◽  
Protein Fusion ◽  
Protein Arrays ◽  
Pou Domain ◽  
Domain Protein ◽  
One To One

Protein array technology has a tremendous potential in large-scale and high-throughput functional proteomics. The conventional protein array approach, which is based on one-to-one indexing between a known protein and an array position, is however, not applicable for decoding genetic information from unknown expressed protein arrays and thus less practicable for global proteome analysis. To address this issue, we have introduced a novel concept of DNA-linked protein array by adopting a strategy of one-to-one indexing between spatially unknown individual DNA arrays and their encoded protein array products. A set of test DNA arrays of DNA-binding (POU) domain protein, c-Myc-tagged POU domain protein, and green fluorescent protein were randomly patterned on a glass surface and subsequently converted into identifiable protein arrays by simultaneously producing and linking the encoded proteins to their encoding genes using a puromycin-assisted mRNA–protein fusion approach in situ. For concept validation, functional assay was performed by the screening of mRNA–protein fusion arrays against a mixture of anti-cMyc and anti-GFP antibodies. Moreover, this approach also provides an additional advantage to incorporate post-translational modifications into protein arrays. The concept developed here is expected to markedly enhance the application of protein microarrays to the study of functional proteomics.

High-Throughput Phosphotyrosine Protein Complexes Screening by Photoaffinity-Engineered Protein Scaffold-Based Forward-Phase Protein Array

2019 ◽  
Vol 91 (15) ◽  
pp. 10026-10032 ◽  
Author(s):  
Zhendong Zheng ◽  
Bizhu Chu ◽  
Qian Kong ◽  
Xiong Chen ◽  
Mi Ke ◽  
...  
Keyword(s):  
High Throughput ◽  
Protein Complexes ◽  
Protein Array ◽  
Protein Scaffold ◽  
Phase Protein

High-Throughput Evaluation of Metabolic Activities Using Reverse Phase Protein Array Technology

2021 ◽  
pp. 293-317
Author(s):  
Hsin-Yi Lu ◽  
Jian Xiong ◽  
Dimuthu Nuwan Perera ◽  
Kimal Rajapakshe ◽  
Xuan Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Protein Array ◽  
Reverse Phase Protein Array ◽  
Reverse Phase ◽  
Array Technology ◽  
Phase Protein ◽  
Metabolic Activities

scholarly journals Integrated Protein Array Screening and High Throughput Validation of 70 Novel Neural Calmodulin-binding Proteins

2010 ◽  
Vol 9 (6) ◽  
pp. 1118-1132 ◽  
Author(s):  
David J. O'Connell ◽  
Mikael C. Bauer ◽  
John O'Brien ◽  
Winifred M. Johnson ◽  
Catherine A. Divizio ◽  
...  
Keyword(s):  
High Throughput ◽  
Binding Proteins ◽  
Protein Array ◽  
Calmodulin Binding

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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