scholarly journals Rapid detection of six common Mediterranean and three non-Mediterranean ?-thalassemia point mutations by reverse dot blot analysis

2003 ◽  
Vol 74 (3) ◽  
pp. 191-195 ◽  
Author(s):  
Enrica Foglietta ◽  
Ida Bianco ◽  
Aurelio Maggio ◽  
Antonino Giambona
Keyword(s):  
Blot Analysis ◽  
Rapid Detection ◽  
Point Mutations ◽  
Dot Blot ◽  
Dot Blot Analysis ◽  
Reverse Dot Blot

Direct sequencing of a novel DPB1 allele (DPB1*5101) of the heterozygote from the membrane of reverse dot blot analysis

1994 ◽  
Vol 44 (3) ◽  
pp. 204-207 ◽  
Author(s):  
Toshihiko Kaneshige ◽  
Tomoko Kinoshita ◽  
Mitsuo Hashimoto ◽  
Yayoi Matsumoto ◽  
Toyoki Moribe ◽  
...  
Keyword(s):  
Blot Analysis ◽  
Direct Sequencing ◽  
Dot Blot ◽  
Dot Blot Analysis ◽  
Reverse Dot Blot

Simultaneous detection of fourteen Italian cystic fibrosis mutations in seven exons by reverse dot-blot analysis

1995 ◽  
Vol 9 (5) ◽  
pp. 357-360 ◽  
Author(s):  
M. Rady ◽  
E. D'Alcamo ◽  
M. Seia ◽  
L. Iapichino ◽  
M. Ferrari ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Blot Analysis ◽  
Simultaneous Detection ◽  
Dot Blot ◽  
Dot Blot Analysis ◽  
Reverse Dot Blot

scholarly journals Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192398 ◽  
Author(s):  
Maite Sabalza ◽  
Rubina Yasmin ◽  
Cheryl A. Barber ◽  
Talita Castro ◽  
Daniel Malamud ◽  
...  
Keyword(s):  
Reverse Transcription ◽  
Blot Analysis ◽  
Zika Virus ◽  
Dot Blot ◽  
Dot Blot Analysis ◽  
Reverse Dot Blot

Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA

2018 ◽  
pp. 263-271 ◽  
Author(s):  
Arvindhan Nagarajan ◽  
Radoslav Janostiak ◽  
Narendra Wajapeyee
Keyword(s):  
Blot Analysis ◽  
Dot Blot ◽  
Dot Blot Analysis

An Improved Method to Do Dot Blot Analysis

1994 ◽  
Vol 222 (1) ◽  
pp. 293-294 ◽  
Author(s):  
K.S. Saini ◽  
B. Bhandari
Keyword(s):  
Blot Analysis ◽  
Improved Method ◽  
Dot Blot ◽  
Dot Blot Analysis

scholarly journals Measurement of telomere DNA content by dot blot analysis

2011 ◽  
Vol 39 (12) ◽  
pp. e84-e84 ◽  
Author(s):  
M. Kimura ◽  
A. Aviv
Keyword(s):  
Dna Content ◽  
Blot Analysis ◽  
Dot Blot ◽  
Dot Blot Analysis

scholarly journals Quantitative dot blot analysis (QDB), a versatile high throughput immunoblot method

Oncotarget ◽  
2017 ◽  
Vol 8 (35) ◽  
pp. 58553-58562 ◽  
Author(s):  
Geng Tian ◽  
Fangrong Tang ◽  
Chunhua Yang ◽  
Wenfeng Zhang ◽  
Jonas Bergquist ◽  
...  
Keyword(s):  
High Throughput ◽  
Blot Analysis ◽  
Dot Blot ◽  
Dot Blot Analysis

HSP-72 synthesis is promoted by increase in [Ca2+]i or activation of G proteins but not pHi or cAMP

1994 ◽  
Vol 267 (1) ◽  
pp. C104-C114 ◽  
Author(s):  
J. G. Kiang ◽  
F. E. Carr ◽  
M. R. Burns ◽  
D. E. McClain
Keyword(s):  
Heat Shock ◽  
G Proteins ◽  
Blot Analysis ◽  
Dot Blot ◽  
Heat Shock Element ◽  
The Family ◽  
Shock Proteins ◽  
Dot Blot Analysis ◽  
The Relationship ◽  
Acid Loading

The family of 70-kDa heat-shock proteins (HSP-70) is evolutionarily highly conserved and has been shown to enhance cell survival from thermal injury. This study characterized HSP-72 induction in human epidermoid A-431 cells exposed to 45 degrees C for 10 min and determined the relationship between HSP-72, intracellular pH (pHi), adenosine 3',5'-cyclic monophosphate (cAMP), G proteins, and intracellular cytosolic free Ca2+ concentration ([Ca2+]i). Heat shock induced HSP-72 production, which was dependent on both temperature and the duration of heating. This HSP-72 induction was confirmed by Western blot analysis. HSP-72 levels in cells that had been heated then returned to 37 degrees C were elevated at 2 h (1.5 +/- 0.1 x control), reached a maximum at 8 h (2.7 +/- 0.1 x control), and remained above baseline for up to 4 days. Levels of HSP-72 mRNA, determined by dot-blot analysis, reached a maximum at 2 h and returned to baseline within 8 h. Both actinomycin D and cycloheximide blocked HSP-72 induction. Because heating causes intracellular acidification and increases in cAMP and [Ca2+]i, we studied the effect of pHi, cellular cAMP, and [Ca2+]i on HSP-72 induction. The reduction of pHi to 6.9 by acid loading did not affect the basal level of HSP-72 in unheated cells. Treatment with pertussis toxin, cholera toxin, or forskolin, but not 8-bromo-cAMP, 3-isobutyl-1-methylxanthine, or N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide potentiated heat-induced HSP-72 production. Inhibition of the heat-induced increase in [Ca2+]i attenuated, but failed to completely block, heat-induced HSP-72 production, mRNA synthesis, and the heat-shock transcriptional factor-heat-shock element binding complex formation, which suggests there are Ca(2+)-dependent and -independent processes involved in HSP-72 synthesis. Our results show that an increase in [Ca2+]i or activation of G proteins, but not pHi and cAMP, enhances HSP-72 induction.

scholarly journals New poly(A)+RNAs appear coordinately during the differentiation of Naegleria gruberi amebae into flagellates.

1986 ◽  
Vol 102 (2) ◽  
pp. 353-361 ◽  
Author(s):  
J Mar ◽  
J H Lee ◽  
D Shea ◽  
C J Walsh
Keyword(s):  
Blot Analysis ◽  
Rna Synthesis ◽  
In Vitro Translation ◽  
Dot Blot ◽  
Cross Hybridization ◽  
Tubulin Mrna ◽  
Dot Blot Analysis ◽  
Rna Concentration ◽  
Naegleria Gruberi

We have examined the nature of the requirement for RNA synthesis during the differentiation of Naegleria gruberi amebae into flagellates (Fulton, C., and C. Walsh, 1980, J. Cell Biol., 85:346-360) by looking for poly(A)+RNAs that are specific to differentiating cells. A cDNA library prepared from poly(A)+RNA extracted from cells 40 min after initiation of the differentiation (40-min RNA), the time when formation of flagella becomes insensitive to inhibitors of RNA synthesis, was cloned into pBR322. Recombinant clones were screened for sequences that were complementary to 40-min RNA but not to RNA from amebae (0-min RNA). Ten of these differentiation-specific (DS) plasmids were identified. The DS plasmids were found to represent at least four different poly(A)+RNAs based on cross-hybridization, restriction mapping, and Northern blot analysis. Dot blot analysis was used to quantify changes in DS RNA concentration. The four DS RNAs appeared coordinately during the differentiation. They were first detectable at 10-15 min after initiation, reached a peak at 70 min as flagella formed, and then declined to low levels by 120 min when flagella reached full length. The concentration of the DS RNAs was found to be at least 20-fold higher in cells at 70 min than in amebae. The changes in DS RNA concentration closely parallel changes in tubulin mRNA as measured by in vitro translation (Lai, E.Y., C. Walsh, D. Wardell, and C. Fulton, 1979, Cell, 17:867-878).

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