scholarly journals Fluorescence in situ hybridization: Method of choice for a definitive diagnosis of mantle cell lymphoma

2003 ◽  
Vol 74 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Tsieh Sun ◽  
Mary Lowery Nordberg ◽  
James D. Cotelingam ◽  
Diana M. Veillon ◽  
John Ryder
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mantle Cell Lymphoma ◽  
Cell Lymphoma ◽  
Definitive Diagnosis ◽  
Hybridization Method ◽  
Mantle Cell

Molecular diagnosis of t(11;14) in mantle cell lymphoma using two-colour interphase fluorescence in situ hybridization

1996 ◽  
Vol 93 (3) ◽  
pp. 656-660 ◽  
Author(s):  
MICHELE MONTEIL ◽  
MARY CALLANAN ◽  
CRISTINA DASCALESCU ◽  
JEAN-JACQUES SOTTO ◽  
DOMINIQUE LEROUX
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mantle Cell Lymphoma ◽  
Molecular Diagnosis ◽  
Cell Lymphoma ◽  
Mantle Cell

scholarly journals Visualization of mono-allelic chromosomal aberrations 3′ and 5′ of the cyclin D1 gene in mantle cell lymphoma using DNA fiber fluorescence in situ hybridization

Oncogene ◽  
1997 ◽  
Vol 15 (13) ◽  
pp. 1599-1603 ◽  
Author(s):  
Carla J de Boer ◽  
Jan-Willem Vaandrager ◽  
Johan HJM van Krieken ◽  
Zandra Holmes ◽  
Philip M Kluin ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cyclin D1 ◽  
Mantle Cell Lymphoma ◽  
Chromosomal Aberrations ◽  
Cell Lymphoma ◽  
Mantle Cell

scholarly journals Detection of Translocation t(11;14)(q13;q32) in Mantle Cell Lymphoma by Fluorescence in Situ Hybridization

1999 ◽  
Vol 154 (5) ◽  
pp. 1449-1452 ◽  
Author(s):  
Jian-Yong Li ◽  
Fanny Gaillard ◽  
Anne Moreau ◽  
Jean-Luc Harousseau ◽  
Christian Laboisse ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mantle Cell Lymphoma ◽  
Cell Lymphoma ◽  
Mantle Cell

Diagnostic utility of fluorescence in situ hybridization in mantle-cell lymphoma

2000 ◽  
Vol 110 (4) ◽  
pp. 856-862 ◽  
Author(s):  
E. D. Remstein ◽  
P. J. Kurtin ◽  
I. Buño ◽  
R. J. Bailey ◽  
J. Proffitt ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mantle Cell Lymphoma ◽  
Cell Lymphoma ◽  
Diagnostic Utility ◽  
Mantle Cell

Flow Cytometry and Fluorescence In Situ Hybridization Are Methods of Choice for Routine Diagnosis of Mantle Cell Lymphoma.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4659-4659 ◽  
Author(s):  
Grzegorz Rymkiewicz ◽  
Renata Woroniecka ◽  
Katarzyna Blachnio ◽  
Barbara Pienkowska-Grela ◽  
Jan Walewski
Keyword(s):  
Flow Cytometry ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cyclin D1 ◽  
Mantle Cell Lymphoma ◽  
Cell Lymphoma ◽  
Initial Diagnosis ◽  
Combined Use ◽  
Mantle Cell

Abstract Mantle cell lymphoma (MCL) is a distinct clinicopathologic entity, characterized by expansion of lymphocytes with co-expression of CD5 and CD20 and frequent t(11;14) translocation. MCL and its morphological variants are frequently confused with other lymphoma subtypes. The aim of this study was to analyze a contribution of histopathology (HP), immunohistochemistry (IHC), flow cytometry (FCM) and cytogenetic analysis with fluorescence in situ hybridization (FISH) to ultimate diagnosis of MCL. We identified 66 pts diagnosed with MCL either by use of HP/IHC or/and FCM. Initial diagnosis was based on HP/IHC only, and was validated in 55 of 66 patients by combined use of HP/IH, FCM, and FISH. We examined paraffin sections IHC panel consisting of antibodies to CD3, CD5, CD20, CD23, CD43, and cyclin D1. FCM analysis was done by 3-colour direct immunofluorescence with extended panel of antibodies to CD5, CD10, CD11c, CD19, CD20, CD22, CD23, CD25, CD38, CD45, HLADR, FMC7, light and heavy chains. We used FISH to detect the t(11;14) in interphase cells of 24 cases of MCL. All 55 cases of MCL were CD20 positive and CD23 negative on IHC, 32 of 51 (63%) and 46 of 52 (88%) cases coexpressed the CD5 and CD43 antigens, respectively. Cyclin-D1 staining revealed nuclear positivity in 38 of 52 (73%) pts. Dual expression of CD5 and cyclin D1 - a finding highly reliable for MCL diagnosis on IHC, was seen in 49% (27/55) of pts. All MCL cases were CD20 (51/51) and CD19 (55/55) positive by FCM with higher intensity of CD20 expression compared to CD19 in 100% (51/51) of cases. CD5 and CD25 were coexpressed in 54 of 55 (98%) and in 43 of 49 (88%) pts respectively. CD22 (45/45) and HLADR (55/55) were positive in all cases. FMC7 and CD38 were found in 22 of 23 (96%) and in 19 of 23 (83%) patients, respectively. CD10, CD11c, CD23 were only seen on a subpopulation of cells with weak intensity in 15%(7/48), 13% (6/45), and 11% (5/46) pts., respectively. MCL cells expressed moderate intensity monoclonal surface light chains in 47 of 52 (90%) pts with L light chain predominance and IgM+/IgD+ in 16 of 17 (94%) pts. False or incomplete initial diagnosis based mainly on lymph node HP/IHC was found in 35 of 66 (53%) cases. False FCM diagnosis of MCL was found in 2 of 66 (3%) cases. Results of FCM and FISH were consistent with the MCL diagnosis in 92% (22/24) of patients, in our hands. Our data indicate that the phenotypic pattern of MCL by FCM is remarkably constant among patients. As MCL represents a frequent diagnostic challenge, a combined use of FCM and FISH may provide an optimum solution.

Secondary Chromosome Changes in Mantle Cell Lymphoma: Cytogenetic and Fluorescence in situ Hybridization Studies

2001 ◽  
Vol 40 (5-6) ◽  
pp. 581-590 ◽  
Author(s):  
Renato Bigoni ◽  
Antonio Cuneo ◽  
Raffaella Milani ◽  
Maria Grazia Roberti ◽  
Antonella Bardi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mantle Cell Lymphoma ◽  
Cell Lymphoma ◽  
Mantle Cell
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