scholarly journals Stage- and region-dependent responses of chick wing-bud mesenchymal cells to retinoic acid in serum-free microcultures

1994 ◽  
Vol 201 (4) ◽  
pp. 310-323 ◽  
Author(s):  
Douglas F. Paulsen ◽  
Wei-Dang Chen ◽  
David Okello ◽  
Benita Johnson
Keyword(s):  
Retinoic Acid ◽  
Mesenchymal Cells ◽  
Serum Free ◽  
Wing Bud

Stable, position-related responses to retinoic acid by chick limb-bud mesenchymal cells in serum-free cultures

1994 ◽  
Vol 30 (3) ◽  
pp. 181-186 ◽  
Author(s):  
Douglas F. Paulsen ◽  
Michael Solursh ◽  
Robert M. Langille ◽  
Lei Pang ◽  
Wei-Dang Chen
Keyword(s):  
Retinoic Acid ◽  
Mesenchymal Cells ◽  
Limb Bud ◽  
Serum Free ◽  
Stable Position

Decalpenic acid induces early osteoblastic markers in pluripotent mesenchymal cells via activation of retinoic acid receptor γ

2012 ◽  
Vol 422 (4) ◽  
pp. 751-757 ◽  
Author(s):  
Shuichi Sakamoto ◽  
Fukiko Kojima ◽  
Isao Momose ◽  
Manabu Kawada ◽  
Hayamitsu Adachi ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Retinoic Acid Receptor ◽  
Mesenchymal Cells ◽  
Acid Receptor

Vitamin A alters the internal viscosity of fragments of limb-bud mesenchyme

Development ◽  
1980 ◽  
Vol 59 (1) ◽  
pp. 325-339
Author(s):  
T. E. Kwasigroch ◽  
D. M. Kochhar
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Mesenchymal Cells ◽  
Limb Bud ◽  
Mouse Limb ◽  
Vitamin A Acid ◽  
Internal Viscosity ◽  
Fusion Experiments

Two techniques were used to examine the effect of vitamin A compounds (vitamin A acid = retinoic acid and vitamin A acetate) upon the relative strengths of adhesion among mouse limb-bud mesenchymal cells. Treatment with retinoic acid in vivo and with vitamin A acetate in vitro reduced the rate at which the fragments of mesenchyme rounded-up when cultured on a non-adhesive substratum, but these compounds did not alter the behavior of tissues tested in fragment-fusion experiments. These conflicting results indicate that the two tests measure different activities of cells and suggest that treatment with vitamin A alters the property(ies) of cells which regulate the internal viscosity of tissues.

Retinoic acid signaling is required during early chick limb development

Development ◽  
1996 ◽  
Vol 122 (5) ◽  
pp. 1385-1394 ◽  
Author(s):  
J.A. Helms ◽  
C.H. Kim ◽  
G. Eichele ◽  
C. Thaller
Keyword(s):  
Retinoic Acid ◽  
Limb Development ◽  
Acid Synthesis ◽  
Apical Ectodermal Ridge ◽  
Limb Bud ◽  
Retinoid Receptor ◽  
Limb Morphogenesis ◽  
Wing Bud ◽  
Zone Of Polarizing Activity

In the chick limb bud, the zone of polarizing activity controls limb patterning along the anteroposterior and proximodistal axes. Since retinoic acid can induce ectopic polarizing activity, we examined whether this molecule plays a role in the establishment of the endogenous zone of polarizing activity. Grafts of wing bud mesenchyme treated with physiologic doses of retinoic acid had weak polarizing activity but inclusion of a retinoic acid-exposed apical ectodermal ridge or of prospective wing bud ectoderm evoked strong polarizing activity. Likewise, polarizing activity of prospective wing mesenchyme was markedly enhanced by co-grafting either a retinoic acid-exposed apical ectodermal ridge or ectoderm from the wing region. This equivalence of ectoderm-mesenchyme interactions required for the establishment of polarizing activity in retinoic acid-treated wing buds and in prospective wing tissue, suggests a role of retinoic acid in the establishment of the zone of polarizing activity. We found that prospective wing bud tissue is a high-point of retinoic acid synthesis. Furthermore, retinoid receptor-specific antagonists blocked limb morphogenesis and down-regulated a polarizing signal, sonic hedgehog. Limb agenesis was reversed when antagonist-exposed wing buds were treated with retinoic acid. Our results demonstrate a role of retinoic acid in the establishment of the endogenous zone of polarizing activity.

scholarly journals The Neurotrophic Function of Glucagon-Like Peptide-1 Promotes Human Neuroblastoma Differentiation via the PI3K-AKT Axis

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 348
Author(s):  
Jenq-Lin Yang ◽  
Yu-Ting Lin ◽  
Wei-Yu Chen ◽  
Yun-Ru Yang ◽  
Shu-Fang Sun ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Patch Clamp ◽  
Neurotrophic Factors ◽  
Human Neuroblastoma ◽  
Nicotinic Acetylcholine ◽  
Serum Free ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1 ◽  
Sugar Levels ◽  
Synapsin 1

Background: Neurons are terminally-differentiated cells that generally develop from neuronal stem cells stimulated by various neurotrophic factors such as NGF, BDNF, NT3, and NT-4. Neurotrophic factors have multiple functions for neurons, including enabling neuronal development, growth, and protection. Glucagon-like peptide-1 (GLP-1) is an intestinal-secreted incretin that enhances cellular glucose up-take to decrease blood sugar levels. However, many studies suggest that the function of GLP-1 is not limited to the regulation of blood sugar levels. Instead, it may also act as a neurotrophic factor with a role in ensuring neuronal survival and neurite outgrowth, as well as protecting synaptic plasticity and memory formation. Methods: The SH-SY5Y cells were differentiated by sequential treatments of retinoic acid and GLP-1 treatment within polyethylenimine-coated dishes under serum-free Neurobasal medium. PI3K inhibitor (LY294002) and MEK inhibitor (U0126) were used to determine the signaling pathway in regulation of neuronal differentiation. Neuronal marker (TUJ1) and synaptic markers (synapsin 1, synaptophysin, and PSD95) as well as single cell patch-clamp were applied to determine maturity of neurons. Antibodies of AMPA receptor, NMDA receptor subunit 2A, dopamine receptor D1, muscarinic acetylcholine receptor 2, and nicotinic acetylcholine receptor α4 were used to examine the types of differentiated neurons. Results: Our study’s results demonstrated that the treatment with GLP-1 of SH-SY5Y human neuroblastoma cells increased the expression of AMPA receptors, NMDA receptors, dopamine receptors, synaptic proteins-synapsin 1, synaptophysin, and postsynaptic density protein 95, but not muscular and nicotinic acetylcholine receptors. In addition, the biomarker of dividing neuronal cells, vimentin, was decreased after treatment with GLP-1. Tuj1 immunostaining images showed that GLP-1 induced neurite processes and the development of neuronal morphologies. The GLP-1-differentiated neurons were able to be induced to generate action potentials by single cell patch-clamp. Our study also suggested that the PI3K-AKT axis is the dominant signaling pathway promoting the differentiation of SH-SY5Y cells into mature and functional neurons in response to GLP-1 receptor activation. Conclusions: The sequential treatment of retinoic acid and GLP-1 within a serum-free medium is able to trigger the differentiation of SH-SY5Y cells into morphologically and physiologically mature glutamatergic and dopaminergic neurons.

Cholera toxin and retinoic acid slow the growth rate of human keratinocytes in low-calcium, serum-free culture

2004 ◽  
Vol 34 (1) ◽  
pp. 59-61
Author(s):  
Danielle Pluff ◽  
William R Dunham ◽  
Cynthia L Marcelo ◽  
Hiromi Kageyama
Keyword(s):  
Growth Rate ◽  
Retinoic Acid ◽  
Cholera Toxin ◽  
Human Keratinocytes ◽  
Serum Free ◽  
Low Calcium
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