scholarly journals Time-lapse total internal reflection fluorescence video of acetylcholine receptor cluster formation on myotubes

1994 ◽  
Vol 201 (1) ◽  
pp. 29-40 ◽  
Author(s):  
Michelle Dong Wang ◽  
Daniel Axelrod
Keyword(s):  
Acetylcholine Receptor ◽  
Total Internal Reflection ◽  
Cluster Formation ◽  
Time Lapse ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Receptor Cluster ◽  
Acetylcholine Receptor Cluster

scholarly journals Imaging Early Steps of Sindbis Virus Infection by Total Internal Reflection Fluorescence Microscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Youling Gu ◽  
Yuanzheng Yang ◽  
Yuechueng Liu
Keyword(s):  
Fluorescence Microscopy ◽  
Total Internal Reflection ◽  
Sindbis Virus ◽  
Time Lapse ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Host Cells ◽  
Broad Host Range ◽  
Early Endosomes ◽  
And Behavior

Sindbis virus (SINV) is an alphavirus that has a broad host range and has been widely used as a vector for recombinant gene transduction, DNA-based vaccine production, and oncolytic cancer therapy. The mechanism of SINV entry into host cells has yet to be fully understood. In this paper, we used single virus tracking under total internal reflection fluorescence microscopy (TIRFM) to investigate SINV attachment to cell surface. Biotinylated viral particles were labeled with quantum dots, which retained viral viability and infectivity. By time-lapse imaging, we showed that the SINV exhibited a heterogeneous dynamics on the surface of the host cells. Analysis of SINV motility demonstrated a two-step attachment reaction. Moreover, dual color TIRFM of GFP-Rab5 and SINV suggested that the virus was targeted to the early endosomes after endocytosis. These findings demonstrate the utility of quantum dot labeling in studying the early steps and behavior of SINV infection.

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