Development of Sertoli cell junctional specializations and the distribution of the tight-junction-associated protein ZO-1 in the mouse testis

1991 ◽  
Vol 191 (1) ◽  
pp. 35-47 ◽  
Author(s):  
Stephen Byers ◽  
Robert Graham ◽  
Hai Ning Dai ◽  
Becky Hoxter
Keyword(s):  
Tight Junction ◽  
Sertoli Cell ◽  
Mouse Testis

Developmental stage- and spermatogenic cycle-specific expression of transcription factor GATA-1 in mouse Sertoli cells

Development ◽  
1994 ◽  
Vol 120 (7) ◽  
pp. 1759-1766 ◽  
Author(s):  
K. Yomogida ◽  
H. Ohtani ◽  
H. Harigae ◽  
E. Ito ◽  
Y. Nishimune ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Developmental Stage ◽  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Transcriptional Activation ◽  
Germ Line ◽  
Mouse Testis ◽  
Seminiferous Tubules ◽  
Specific Expression

GATA-1 is an essential factor for the transcriptional activation of erythroid-specific genes, and is also abundantly expressed in a discrete subset of cells bordering the seminiferous epithelium in tubules of the murine testis. In examining normal and germ-line defective mutant mice, we show here that GATA-1 is expressed only in the Sertoli cell lineage in mouse testis. GATA-1 expression in Sertoli cells is induced concomitantly with the first wave of spermatogenesis, and GATA-1-positive cells are uniformly distributed among all tubules during prepubertal testis development. However, the number of GATA-1-positive cells declines thereafter and were found only in the peripheral zone of seminiferous tubules in stages VII, VIII and IX of spermatogenesis in the adult mouse testis. In contrast, virtually every Sertoli cell in mutant W/Wv, jsd/jsd or cryptorchid mice (all of which lack significant numbers of germ cells) expresses GATA-1, thus showing that the expression of this transcription factor is negatively controlled by the maturing germ cells. These observations suggest that transcription factor GATA-1 is a developmental stage- and spermatogenic cycle-specific regulator of gene expression in Sertoli cells.

scholarly journals Retinoblastoma protein represses E2F3 to maintain Sertoli cell quiescence in mouse testis

2019 ◽  
Vol 132 (14) ◽  
pp. jcs229849 ◽  
Author(s):  
Emmi Rotgers ◽  
Sheyla Cisneros-Montalvo ◽  
Mirja Nurmio ◽  
Jorma Toppari
Keyword(s):  
Sertoli Cell ◽  
Retinoblastoma Protein ◽  
Mouse Testis ◽  
Cell Quiescence

scholarly journals NRG1 signalling regulates the establishment of Sertoli cell stock in the mouse testis

2018 ◽  
Vol 478 ◽  
pp. 17-31 ◽  
Author(s):  
Elodie P. Gregoire ◽  
Isabelle Stevant ◽  
Anne-Amandine Chassot ◽  
Luc Martin ◽  
Simon Lachambre ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Mouse Testis

scholarly journals Expression of E-Cadherin in Immature Rat and Mouse Testis and in Rat Sertoli Cell Cultures1

1993 ◽  
Vol 49 (6) ◽  
pp. 1353-1361 ◽  
Author(s):  
Jiahn-Chun Wu ◽  
Christopher W. Gregory ◽  
Robert M. DePhilip
Keyword(s):  
Sertoli Cell ◽  
Mouse Testis ◽  
Immature Rat ◽  
E Cadherin

scholarly journals CHANGE OF LANTHANUM PERMEABILITY OF RAT SERTOLI CELL TIGHT JUNCTION AFTER CDDP ADMINISTRATION

1990 ◽  
Vol 81 (1) ◽  
pp. 45-48 ◽  
Author(s):  
Shuichi Gotoh ◽  
Masahide Mine ◽  
Kazuhiro Ishizaka ◽  
Humihisa Kaneoya ◽  
Masayuki Yokokawa
Keyword(s):  
Tight Junction ◽  
Sertoli Cell

Sertoli cell replacement in explanted mouse testis tissue supporting host spermatogenesis

2021 ◽  
Author(s):  
Kazusa Higuch ◽  
Takafumi Matsumura ◽  
Haruhiko Akiyama ◽  
Yoshiakira Kanai ◽  
Takehiko Ogawa ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Germ Cells ◽  
Sertoli Cells ◽  
Diphtheria Toxin ◽  
Mouse Testis ◽  
Seminiferous Tubules ◽  
Functional Assay ◽  
Cell Replacement ◽  
Replacement Method ◽  
Toxin Receptor

Abstract Spermatogenesis takes place in the seminiferous tubules, starting from the spermatogonial stem cell and maturing into sperm through multiple stages of cell differentiation. Sertoli cells, the main somatic cell constituting the seminiferous tubule, are in close contact with every germ cell and play pivotal roles in the progression of spermatogenesis. In this study, we developed an in vitro Sertoli cell replacement method by combining an organ culture technique and a toxin receptor-mediated cell knockout (Treck) system. We used Amh- diphtheria toxin receptor (DTR) transgenic mice, whose Sertoli cells specifically express human DTR, which renders them sensitive to diphtheria toxin (DT). An immature Amh-DTR testis was transplanted with donor testis cells followed by culturing in a medium containing DT. This procedure successfully replaced the original Sertoli cells with the transplanted Sertoli cells, and spermatogenesis originating from resident germ cells was confirmed. In addition, Sertoli cells in the mouse testis tissues were replaced by transplanted rat Sertoli cells within culture conditions, without requiring immunosuppressive treatments. This method works as a functional assay system, making it possible to evaluate any cells that might function as Sertoli cells. It would also be possible to investigate interactions between Sertoli and germ cells more closely, providing a new platform for the study of spermatogenesis and its impairments.

scholarly journals Growth Differentiation Factor 9 Is a Germ Cell Regulator of Sertoli Cell Function

Endocrinology ◽  
2008 ◽  
Vol 150 (5) ◽  
pp. 2481-2490 ◽  
Author(s):  
Peter K. Nicholls ◽  
Craig A. Harrison ◽  
Robert B. Gilchrist ◽  
Paul G. Farnworth ◽  
Peter G. Stanton
Keyword(s):  
Tight Junction ◽  
Germ Cell ◽  
Sertoli Cell ◽  
Cell Cultures ◽  
Seminiferous Epithelium ◽  
Inhibin B ◽  
Receptor Type ◽  
Type Ii ◽  
Bmp Receptor ◽  
Differentiation Factor

Oocyte-secreted growth differentiation factor (GDF) 9 and bone morphogenetic protein (BMP) 15 are critical regulatory factors in female reproduction. Together, they promote granulosa cell proliferation and stimulate the maturation of preovulatory follicles. Despite their importance in female fertility, GDF9 and BMP15 expression patterns and function during spermatogenesis have not been investigated. In this study we show that the expression and stage-specific localization of both factors are limited to the germ cells of the rat seminiferous epithelium, with GDF9 being principally localized in round spermatids and BMP15 in gonocytes and pachytene spermatocytes. To identify potential cellular targets for GDF9 actions, cells of the seminiferous tubule were isolated and screened for the expression of signaling receptors [activin-like kinase (ALK) 5, ALK6, and BMP receptor, type II)]. Individual receptor types were expressed throughout the seminiferous epithelium, but coexpression of ALK5 and BMP receptor, type II was limited to Sertoli cells and round spermatids. Based on the reproductive actions of related TGFβ ligands in the ovary and testis, GDF9 was assessed for its ability to regulate tight junction function and inhibin B production in rat Sertoli cell cultures. When recombinant mouse GDF9 was added to immature Sertoli cell cultures, it inhibited membrane localization of the junctional proteins claudin-11, occludin, and zonula occludens-1, thereby disrupting tight junction integrity. Concomitantly, GDF9 up-regulated inhibin subunit expression and significantly stimulated dimeric inhibin B protein production. Together, these results demonstrate that GDF9 and BMP15 are germ cell-specific factors in the rat testis, and that GDF9 can modulate key Sertoli cell functions.

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