Distribution and movement of membrane-associated platelet glycoproteins: Use of colloidal gold with correlative video-enhanced light microscopy, low-voltage high-resolution scanning electron microscopy, and high-voltage transmission electron microscopy

1989 ◽  
Vol 185 (2-3) ◽  
pp. 149-164 ◽  
Author(s):  
Ralph M. Albrecht ◽  
Steven L. Goodman ◽  
Scott R. Simmons
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
High Voltage ◽  
Colloidal Gold ◽  
Low Voltage ◽  
Transmission Electron ◽  
Scanning Electron

scholarly journals Optimizing parameters for correlative immunogold localization by video-enhanced light microscopy, high-voltage transmission electron microscopy, and field emission scanning electron microscopy.

1990 ◽  
Vol 38 (12) ◽  
pp. 1781-1785 ◽  
Author(s):  
S R Simmons ◽  
J B Pawley ◽  
R M Albrecht
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
High Voltage ◽  
Low Voltage ◽  
Immunogold Localization ◽  
Transmission Electron ◽  
High Voltage Transmission ◽  
Scanning Electron

Correlative video-enhanced light microscopy, high-voltage transmission electron microscopy, and low-voltage high resolution scanning electron microscopy were used to examine the binding of colloidal gold-labeled fibrinogen to platelet surfaces. Optimal conditions for the detection of large (18 nm) and small (3 nm) gold particles are described.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
The Other ◽  
Transmission Electron ◽  
Palm Species ◽  
Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 

Radiation – induced preparation of polyaniline/poly vinyl alcohol nanocomposites and their properties

2019 ◽  
Vol 107 (8) ◽  
pp. 725-735
Author(s):  
Hoda H. Saleh ◽  
Rehab Sokary ◽  
Zakaria I. Ali
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Vinyl Alcohol ◽  
Oxidative Polymerization ◽  
Nanocomposite Films ◽  
Poly Vinyl Alcohol ◽  
Transmission Electron ◽  
Scanning Electron

Abstract Polyaniline (PANI) nanoparticles and PANI/poly vinyl alcohol (PVA) nanocomposite films were synthesized by the oxidative polymerization of aniline and ammonium peroxodisulfate (APS), as an oxidizing agent in aqueous medium. The PANI/PVA nanocomposite films were exposed to γ-irradiation after oxidative polymerization. Synthesized polyaniline (PANI) nanoparticles and PANI/PVA nanocomposite films were characterized by attenuated total reflectance infrared spectroscopy (FTIR-ATR), X-ray diffraction, high resolution scanning electron microscopy, (HRSEM) high resolution transmission electron microscopy, (HRTEM) and UV-VIS absorption spectroscopy. Energy band gap of PANI nanofibers was determined from Tauc’s plots which equal 4.2 eV. Scanning electron microscopy images show that chemically synthesized of polyaniline has nanofibers structure and irradiated PANI/PVA nanocomposite have a mixture of nanorod and nanosphere structures. The transmission electron microscopy show that chemically synthesized of polyaniline has average length in the range 34 ± 10 nm with less wide distribution, where as the irradiated PANI/PVA nanocomposite has coreshell structure.

Colonization of Sphagnum cells by Lyophyllum palustre

1985 ◽  
Vol 63 (4) ◽  
pp. 757-761 ◽  
Author(s):  
E. Untiedt ◽  
K. Müller
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Host Cell ◽  
Cell Walls ◽  
Transmission Electron ◽  
Scanning Electron

Lyophyllum palustre (Peck) Singer, a basidiomycete (Tricholomataceae) parasitizing Sphagnum, was examined for points of contact between hyphae and Sphagnum cells with the help of light microscopy, scanning electron microscopy, and transmission electron microscopy. Results indicate that the fungus attacks Sphagnum cells by penetrating cell walls and altering host cell protosplasm. In addition, the formation of additional partitioning cell walls in attacked living Sphagnum cells was observed.

scholarly journals Mitochondria of Human Adrenal Cortex Have Tubular Cristae with Bulbous Tips

2003 ◽  
Vol 88 (4) ◽  
pp. 1903-1906 ◽  
Author(s):  
Alessandro Riva ◽  
Felice Loffredo ◽  
Alessandro Uccheddu ◽  
Francesca Testa Riva ◽  
Bernard Tandler
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Adrenal Cortex ◽  
Thin Sections ◽  
Transmission Electron ◽  
Soluble Material ◽  
Bulbous Tip ◽  
Scanning Electron

By taking advantage of a modified osmium maceration technique, we have been able to examine by high resolution scanning electron microscopy (HRSEM) the interior of human adrenocortical mitochondria from which all soluble material has been extracted. The so-called vesicles apparent in thin sections examined by transmission electron microscopy actually are finger-like cristae as determined by HRSEM. These digitiform cristae have a segmented appearance and a bulbous tip. The segmented form of the cristae may have important metabolic implications.

Sign in / Sign up

Export Citation Format

Share Document