Fluctuations in follicular structures of rat thyroid glands during 24 hours: Fine structural and morphometric studies

1986 ◽  
Vol 175 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Yasuo Uchiyama ◽  
Akemi Oomiya ◽  
Gen Murakami
Keyword(s):  
Thyroid Glands ◽  
Rat Thyroid

scholarly journals ENGLISH VERSION: AGE FEATURES OF RADIOACTIVE IODINE (131I) ABSORPTION BY RAT THYROID GLANDS IN CORRECTION OF THE DIETARY IODINE DEFICIENCY WITH ORGANIC IODINE

2021 ◽  
Vol 25 (1-2) ◽  
pp. 31-35
Author(s):  
O.I. Ryabukha
Keyword(s):  
Thyroid Gland ◽  
Iodine Deficiency ◽  
Radioactive Iodine ◽  
Male Rats ◽  
Organic Iodine ◽  
Study Results ◽  
Thyroid Glands ◽  
Immature Rats ◽  
Old Rats ◽  
Rat Thyroid

The structure of endocrine morbidity is characterized by a significant spread of thyroid pathology. The insufficient efficacy of inorganic iodine drugs poses the problem of search for new means for iodine deficiency treatment and prevention. Given the progressive aging of the population in economically developed countries, the purpose of the study was to clarify the effect of organic iodine on the features of absorption and elimination of radioactive iodine from the thyroid glands of variously aged rats in the conditions of iodine deficiency in the diet. The study was performed on nonlinear white male rats in two series of studies that were kept on iodine-deficient isocaloric starch-casein diet for 60 days: the first series included two groups of old rats weighing 0.400-0.450 kg, the second series – two groups of sexually immature rats weighing 0.060-0.090 kg. There were 5 rats in each group. In animals of the experimental groups in each series, 10% of casein in the diet was replaced with organic iodine, which came with iodine-protein preparation from the red Black Sea algae Phyllophora nervosa. The functional state of the thyroid gland was studied using the Sodium Iodide Na 131 I Injection drug. The dosimetry was performed using the STS-6 Geiger-Muller Detector. Radioindication of the thyroid gland was carried out after subcutaneous administration of 0.1 ml of 131I solution at the following time intervals: 0.5, 1, 2, 4, 6, 8, 10, 12, 24, 48, 72, and 96 hours after administration of 131I. The study results were presented as a percentage of the radioiodine dose administered, adjusted for natural radioactivity background and the radioactive decay of the drug. It was found that in the iodine deficiency conditions, the thyroid glands of old rats have higher rates of radioiodine absorption and a lower rate of its excretion than the glands of immature rats, which indicates their lower iodine reserve and greater liability to iodine deficiency pathology. Intake of organic iodine regardless of the rats’ age is accompanied by a decrease in radioiodine accumulation and acceleration of its excretion from the thyroid gland, which indicates a decrease in functional stress, but the glands of older rats absorb more iodine and excrete it more slowly, indicating less effective correction of iodine deficiency with age. Reduced functional activity of the thyroid glands in old rats can be used as a sensitive changes marker for the in-depth study of thyrotropic and thyroid disrupting effects.

scholarly journals DETERMINATION OF IODINE CONCENTRATION AND DISTRIBUTION IN RAT THYROID FOLLICLES BY ELECTRON-PROBE MICROANALYSIS

1969 ◽  
Vol 43 (1) ◽  
pp. 115-121 ◽  
Author(s):  
William L. Robison ◽  
David Davis
Keyword(s):  
Sample Preparation ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Freeze Drying ◽  
Iodine Concentration ◽  
Sodium Concentration ◽  
Thyroid Glands ◽  
The Mean ◽  
Rat Thyroid

The concentration and the distribution of iodine in various sized follicles of rat thyroid glands have been analyzed by electron-probe microanalysis. The results of the iodine analysis were grouped according to uncorrected lumen diameter size. No significant differences in iodine concentration were observed among the various size categories. When the results for all follicles from a given sample were pooled, the standard error of the mean was approximately 4%. Usually 40–50 follicles per animal were analyzed. The concentration of iodine ranged from 0.9 to 2.1% by weight among individual animals. Scanning pictures and step-scan analysis showed the iodine distribution to be quite uniform across the colloid area. Several techniques of sample preparation were used; they produced no significant differences in the iodine concentrations observed. Sodium concentration, also determined in all samples, was found to vary from 2 to 9% by weight. Because of the mobility of the sodium ion, its distribution was greatly affected by the method of sample preparation. The technique that best preserved the natural chemistry of the sample was that of freezing the tissue, sectioning, and then freeze-drying.

WATER AND ELECTROLYTE DISTRIBUTION IN RAT AND GUINEA-PIG THYROID GLANDS

1971 ◽  
Vol 50 (4) ◽  
pp. 561-576 ◽  
Author(s):  
S. Y. CHOW ◽  
D. M. WOODBURY
Keyword(s):  
Thyroid Gland ◽  
Guinea Pig ◽  
Interstitial Fluid ◽  
Equilibrium Potential ◽  
Excellent Correlation ◽  
Nernst Equation ◽  
Thyroid Glands ◽  
Uptake Studies ◽  
Rat Thyroid ◽  
Cellular Compartments

SUMMARY Procedures for the calculation of the volume of water and the Na+, K+ and Cl− concentrations in the three anatomical compartments of the thyroid (interstitium, follicular cells and lumen) are described. There was an excellent correlation between the volume of water of these three compartments calculated from the total water, [14C]inulin space and histometric measurements of the thyroid gland and the results obtained from 3H2O-uptake studies. Na+ and K+ concentrations in the luminal fluid of rat and guinea-pig thyroid glands were the same as those in the interstitial fluid which is an ultrafiltrate of plasma. Cl− concentration in the luminal fluid of thyroid gland was usually lower than that in the interstitial fluid. The concentration depends on the amount of colloid present in the follicular lumen. In the cellular fluid of the thyroid follicle, Na+ and Cl− concentrations were low and K+ concentration was high. The Cl− equilibrium potential between the interstitial and the cellular compartments, calculated from the corresponding Cl− concentrations by the Nernst equation, was the same as for previous values reported for the intracellular potentials determined by micro-electrode techniques in both rat and guinea-pig thyroid glands. In the rat thyroid, the calculated Cl− equilibrium potential between the interstitial and the luminal compartments was the same as the measured intra-luminal potential. However, in the guinea-pig thyroid the calculated Cl− equilibrium potential between the interstitial and the luminal fluids was considerably higher than the measured intra-luminal potential.

On the Identification of 4-Hydroxy-3,5-Diiodophenylpyruvic Acid in Rat Thyroid Glands

Endocrinology ◽  
1968 ◽  
Vol 82 (6) ◽  
pp. 1156-1162 ◽  
Author(s):  
MARTIN I. SURKS ◽  
SANDRA WEINBACH ◽  
EUGENE M. VOLPERT
Keyword(s):  
Thyroid Glands ◽  
Rat Thyroid

INABILITY OF HETEROLOGOUS ANTIBODIES TO AFFECT RAT THYROID GLAND

1961 ◽  
Vol 39 (4) ◽  
pp. 691-694 ◽  
Author(s):  
H. S. Sodhi
Keyword(s):  
Thyroid Gland ◽  
Thyroid Antibodies ◽  
Specific Antibodies ◽  
Thyroid Glands ◽  
Rat Thyroid ◽  
Rat Thyroid Gland

Heterologous rat thyroid antibodies produced in rabbits were injected by intraperitoneal, intravenous, and intra-arterial routes in different groups of rats and the effects on the morphology and 24-hour I131 uptake of their thyroid glands were investigated. In spite of the administration of high titers of specific antibodies no effects, acute or chronic, were observed, indicating the inability of the heterologous thyroid antibodies to alter the structure or function of the rat thyroid glands.

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