The plasma membrane and matrix vesicles of mouse growth plate chondrocytes during differentiation as revealed in freeze-fracture replicas

1985 ◽  
Vol 173 (4) ◽  
pp. 269-286 ◽  
Author(s):  
Toshitaka Akisaka ◽  
Carol V. Gay
Keyword(s):  
Plasma Membrane ◽  
Growth Plate ◽  
Freeze Fracture ◽  
Matrix Vesicles ◽  
Growth Plate Chondrocytes

scholarly journals Selective enrichment of microRNAs in extracellular matrix vesicles produced by growth plate chondrocytes

Bone ◽  
2016 ◽  
Vol 88 ◽  
pp. 47-55 ◽  
Author(s):  
Zhao Lin ◽  
Nicholas E. Rodriguez ◽  
Junjun Zhao ◽  
Allison N. Ramey ◽  
Sharon L. Hyzy ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Growth Plate ◽  
Matrix Vesicles ◽  
Growth Plate Chondrocytes ◽  
Selective Enrichment

scholarly journals MicroRNA Contents in Matrix Vesicles Produced by Growth Plate Chondrocytes are Cell Maturation Dependent

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Zhao Lin ◽  
Michael J. McClure ◽  
Junjun Zhao ◽  
Allison N. Ramey ◽  
Niels Asmussen ◽  
...  
Keyword(s):  
Growth Plate ◽  
Matrix Vesicles ◽  
Cell Maturation ◽  
Growth Plate Chondrocytes

Isolation of a plasma membrane-enriched fraction from collagenase-suspended rachitic rat growth plate chondrocytes

1983 ◽  
Vol 1 (3) ◽  
pp. 319-324 ◽  
Author(s):  
William W. Bohn ◽  
Ross M. Stein ◽  
Howard H. T. Hsu ◽  
David C. Morris ◽  
H. Clarke Anderson
Keyword(s):  
Plasma Membrane ◽  
Growth Plate ◽  
Growth Plate Chondrocytes ◽  
Rat Growth

scholarly journals Retinoic acid stimulates annexin-mediated growth plate chondrocyte mineralization

2002 ◽  
Vol 157 (6) ◽  
pp. 1061-1070 ◽  
Author(s):  
Wei Wang ◽  
Thorsten Kirsch
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Retinoic Acid ◽  
Growth Plate ◽  
Calcium Concentration ◽  
Matrix Vesicles ◽  
Cytosolic Calcium ◽  
Annexin Ii ◽  
Growth Plate Chondrocytes ◽  
Cytosolic Calcium Concentration

Biomineralization is a highly regulated process that plays a major role during the development of skeletal tissues. Despite its obvious importance, little is known about its regulation. Previously, it has been demonstrated that retinoic acid (RA) stimulates terminal differentiation and mineralization of growth plate chondrocytes (Iwamoto, M., I.M. Shapiro, K. Yagumi, A.L. Boskey, P.S. Leboy, S.L. Adams, and M. Pacifici. 1993. Exp. Cell Res. 207:413–420). In this study, we provide evidence that RA treatment of growth plate chondrocytes caused a series of events eventually leading to mineralization of these cultures: increase in cytosolic calcium concentration, followed by up-regulation of annexin II, V, and VI gene expression, and release of annexin II–, V–, VI– and alkaline phosphatase–containing matrix vesicles. Cotreatment of growth plate chondrocytes with RA and BAPTA-AM, a cell permeable Ca2+ chelator, inhibited the up-regulation of annexin gene expression and mineralization of these cultures. Interestingly, only matrix vesicles isolated from RA-treated cells that contained annexins, were able to take up Ca2+ and mineralize, whereas vesicles isolated from untreated or RA/BAPTA-treated cells, that contained no or only little annexins were not able to take up Ca2+ and mineralize. Cotreatment of chondrocytes with RA and EDTA revealed that increases in the cytosolic calcium concentration were due to influx of extracellular calcium. Interestingly, the novel 1,4-benzothiazepine derivative K-201, a specific annexin Ca2+ channel blocker, or antibodies specific for annexin II, V, or VI inhibited the increases in cytosolic calcium concentration in RA-treated chondrocytes. These findings indicate that annexins II, V, and VI form Ca2+ channels in the plasma membrane of terminally differentiated growth plate chondrocytes and mediate Ca2+ influx into these cells. The resulting increased cytosolic calcium concentration leads to a further up-regulation of annexin II, V, and VI gene expression, the release of annexin II–, V–, VI– and alkaline phosphatase–containing matrix vesicles, and the initiation of mineralization by these vesicles.

scholarly journals Ultrastructural localization of calcium-activated adenosine triphosphatase (Ca2+-ATPase) in growth-plate cartilage.

1985 ◽  
Vol 33 (9) ◽  
pp. 925-932 ◽  
Author(s):  
T Akisaka ◽  
C V Gay
Keyword(s):  
Plasma Membrane ◽  
Enzymatic Activity ◽  
Growth Plate ◽  
Golgi Complex ◽  
Adenosine Triphosphatase ◽  
Matrix Vesicles ◽  
Matrix Vesicle ◽  
Epiphyseal Growth Plate ◽  
Growth Plate Cartilage ◽  
Vesicle Membranes

The electron-microscopic cytochemical localization of calcium-activated adenosine triphosphatase (Ca2+-ATPase) was determined in chick epiphyseal growth-plate cartilage. In the reserve zone, mitochondria and lysosomes contained substantial amounts of reaction product, while the plasma membrane and the Golgi complex showed very weak enzymatic activity, and matrix vesicle membranes did not exhibit the cytochemical reaction. As maturation proceeded, the plasma membrane, Golgi complex, and matrix vesicle membranes also stained and were most intense in the proliferative and early hypertrophic zones. From the hypertrophic to the calcifying zone, cytochemical staining decreased progressively in the plasma membrane, the Golgi complex, and lysosomes, while in some cases mitochondrial reaction product remained intense. Matrix vesicles lost their enzymatic activity at the same time that matrix vesicle calcification commenced. It is proposed that this event allows matrix vesicles to calcify, since efflux of calcium would no longer occur.

Heterogeneity of Size and Distribution of Intramembrane Particles in the Plasma Membrane of Yeast

1977 ◽  
Vol 35 ◽  
pp. 596-597
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Candida Utilis ◽  
Synthetic Medium ◽  
Freeze Fracture ◽  
Translational Diffusion ◽  
Yeast Cells ◽  
Distilled Water ◽  
Intramembrane Particles ◽  
The Matrix ◽  
Water Cell

The matrix of biological membranes consists of a lipid bilayer into which proteins or protein aggregates are intercalated. Freeze-fracture techni- ques permit these proteins, perhaps in association with lipids, to be visualized in the hydrophobic regions of the membrane. Thus, numerous intramembrane particles (IMP) have been found on the fracture faces of membranes from a wide variety of cells (1-3). A recognized property of IMP is their tendency to form aggregates in response to changes in experi- mental conditions (4,5), perhaps as a result of translational diffusion through the viscous plane of the membrane. The purpose of this communica- tion is to describe the distribution and size of IMP in the plasma membrane of yeast (Candida utilis).Yeast cells (ATCC 8205) were grown in synthetic medium (6), and then harvested after 16 hours of culture, and washed twice in distilled water. Cell pellets were suspended in growth medium supplemented with 30% glycerol and incubated for 30 minutes at 0°C, centrifuged, and prepared for freeze-fracture, as described earlier (2,3).

Sign in / Sign up

Export Citation Format

Share Document