Ultrastructure of cultured atrial cardiac muscle cells from adult rats

1984 ◽  
Vol 171 (2) ◽  
pp. 191-206 ◽  
Author(s):  
R. L. Moses ◽  
William C. Claycomb
Keyword(s):  
Cardiac Muscle ◽  
Muscle Cells ◽  
Adult Rats ◽  
Cardiac Muscle Cells

scholarly journals Proto-oncogene expression in proliferating and differentiating cardiac and skeletal muscle

1987 ◽  
Vol 247 (3) ◽  
pp. 701-706 ◽  
Author(s):  
W C Claycomb ◽  
N A Lanson
Keyword(s):  
Skeletal Muscle ◽  
Cardiac Muscle ◽  
Muscle Tissue ◽  
Cellular Proliferation ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Specific Cell ◽  
Adult Rats ◽  
Cardiac Muscle Cells ◽  
Oncogene Expression

We have examined the expression of 13 proto-oncogenes in proliferating and terminally differentiated cardiac and skeletal muscle. Total RNA was prepared from intact ventricular cardiac-muscle tissue and from purified ventricular cardiac-muscle cells of neonatal and adult rats and from cultured proliferating and terminally differentiated L6A1 rat skeletal-muscle cells. cDNA probes for histone H4, thymidine kinase, myosin heavy chain and M-creatine kinase were used to assess cellular proliferation and differentiation. Oncogenes c-myc, c-raf, c-erb-A, c-ras-H, c-ski, and c-sis were expressed in both proliferating and differentiated cardiac muscle tissue and cells, whereas c-myb expression was not observed in either. c-src was expressed only in neonatal cardiac muscle tissue and cells. c-fms, c-abl, and c-ras-K were expressed in tissue from both neonatal and adult animals but only in purified cells from neonatal animals. c-fes/fps was expressed only in neonatal cardiac muscles cells. c-fos expression was not observed in cardiac-muscle tissue from either neonatal or adult rats, but surprisingly was abundantly expressed in freshly isolated cardiac-muscle cells from animals of both ages. These results emphasize that biochemical analysis using intact cardiac-muscle tissue may not necessarily reflect muscle-specific cell processes. They also show that the expression of c-fos can be activated by the cell isolation procedure. c-myc, c-ski, c-ras-H, c-ras-K, c-abl, c-raf and c-erb-A were expressed in both proliferating and terminally differentiated skeletal-muscle cells, whereas c-myb, c-fos, c-src and c-fms transcripts were observed only in proliferating cells. c-fes/fps and c-sis were not expressed in dividing or fused skeletal-muscle cells. These results demonstrate unique tissue and cell-specific patterns of proto-oncogene expression and suggest that these genes may be involved with the regulation of cellular proliferation and terminal differentiation in striated muscle.

scholarly journals Preproenkephalin mRNA expression in developing rat heart and in cultured ventricular cardiac muscle cells

1989 ◽  
Vol 258 (1) ◽  
pp. 73-78 ◽  
Author(s):  
J P Springhorn ◽  
W C Claycomb
Keyword(s):  
Cardiac Muscle ◽  
Cell Cultures ◽  
Translational Regulation ◽  
Muscle Cells ◽  
Heart Cell ◽  
Developmentally Regulated ◽  
Adult Rats ◽  
Cardiac Muscle Cells ◽  
Adult Rat ◽  
Developing Rat

Heart muscle tissue has previously been reported to have the highest content of preproenkephalin (ppEnk) mRNA of any tissue in the adult rat. We have determined that it is present in the ventricular cardiac muscle cells of the heart and is developmentally regulated. The expression of ppEnk mRNA was observed to be low throughout the first 2 weeks of postnatal development and decreases substantially during week 3. Expression was again low by week 4, but by adulthood (approx. 3 months), it reached a maximum. ppEnk mRNA was actively expressed in primary cardiac muscle cell cultures prepared from both neonatal and adult rats. Its steady-state content in cell cultures was observed to be increased by cyclic AMP and 3-isobutyl-1-methylxanthine. The phorbol ester phorbol 12-myristate 13-acetate elicited a transient effect (i.e. an increase was observed at 4 h and a return to control values by 24 h). We speculate that enkephalin may play a multi-functional role in the differentiation of neonatal cardiac muscle cells and in the terminally differentiated adult heart cell. We demonstrate that the primary culture systems employed in this study will be useful models with which to explore both transcriptional and translational regulation of ppEnk mRNA in the heart.

scholarly journals Uniform sarcomere shortening behavior in isolated cardiac muscle cells.

1980 ◽  
Vol 76 (5) ◽  
pp. 587-607 ◽  
Author(s):  
J W Krueger ◽  
D Forletti ◽  
B A Wittenberg
Keyword(s):  
Cardiac Muscle ◽  
Heart Muscle ◽  
Sarcomere Length ◽  
Maximum Velocity ◽  
Diffraction Method ◽  
Muscle Cells ◽  
Adult Rats ◽  
Cardiac Muscle Cells ◽  
Ventricular Tissue ◽  
Intact Heart

We have observed the dynamics of sarcomere shortening and the diffracting action of single, functionally intact, unattached cardiac muscle cells enzymatically isolated from the ventricular tissue of adult rats. Sarcomere length was measured either (a) continuously by a light diffraction method or (b) by direct inspection of the cell's striated image as recorded on videotape or by cinemicroscopy (120--400 frames/s). At physiological levels of added CaCl2 (0.5--2.0 mM), many cells were quiescent (i.e., they did not beat spontaneously) and contracted in response to electrical stimulation (less than or equal to 1.0-ms pulse width). Sarcomere length in the quiescent, unstimulated cells (1.93 +/- 0.10 [SD] micrometers), at peak shortening (1.57 +/- 0.13 micrometers, n = 49), and the maximum velocity of sarcomere shortening and relengthening were comparable to previous observations in intact heart muscle preparations. The dispersion of light diffracted by the cell remained narrow, and individual striations remained distinct and laterally well registered throughout the shortening-relengthening cycle. In contrast, appreciable nonuniformity and internal buckling were seen at sarcomere lengths < 1.8 micrometers when the resting cell, embedded in gelatin, was longitudinally compressed These results indicate (a) that shortening and relengthening is characterized by uniform activation between myofibrils within the cardiac cell and (b) that physiologically significant relengthening forces in living heart muscle originate at the level of the cell rather than in extracellular connections. First-order diffracted light intensity, extremely variable during sarcomere shortening, was always greatest during midrelaxation preceding the onset of a very slow and uniform phase of sarcomere relengthening.

THE EFFECTS OF ACUPUNCTURE ON CARDIAC MUSCLE CELLS AND BLOOD PRESSURE IN SPONTANEOUS HYPERTENSIVE RATS

2004 ◽  
Vol 29 (1) ◽  
pp. 83-95 ◽  
Author(s):  
Hung-Chien Wu ◽  
Jaung-Geng Lin ◽  
Chun-Hsien Chu ◽  
Yung-Hsien Chang ◽  
Chung-Gwo Chang ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Cardiac Muscle ◽  
Muscle Cells ◽  
Hypertensive Rats ◽  
Cardiac Muscle Cells

Dendroaspis Natriuretic Peptide Induces the Apoptosis of Cardiac Muscle Cells

2005 ◽  
Vol 27 (1) ◽  
pp. 33-51 ◽  
Author(s):  
Ki-Chan Ha ◽  
Han-Jung Chae ◽  
Cheng-Shi Piao ◽  
Suhn-Hee Kim ◽  
Hyung-Ryong Kim ◽  
...  
Keyword(s):  
Cardiac Muscle ◽  
Natriuretic Peptide ◽  
Muscle Cells ◽  
Cardiac Muscle Cells

Microwave Radiation Effects on Cardiac Muscle Cells in Vitro

1981 ◽  
Vol 86 (2) ◽  
pp. 358 ◽  
Author(s):  
M. J. Galvin ◽  
C. A. Hall ◽  
D. I. McRee
Keyword(s):  
Cardiac Muscle ◽  
Microwave Radiation ◽  
Radiation Effects ◽  
Muscle Cells ◽  
Cardiac Muscle Cells
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