Three-dimensional characteristics of the endoplasmic reticulum in the columnar cells of the rat small interstine: An electron microscopy study in thick sections

1983 ◽  
Vol 167 (4) ◽  
pp. 479-493 ◽  
Author(s):  
G. Thiéry ◽  
P. Gaffiero ◽  
M. Bergeron
2013 ◽  
Vol 24 (6) ◽  
pp. 1579-1588 ◽  
Author(s):  
Angel Merchán-Pérez ◽  
José-Rodrigo Rodríguez ◽  
Santiago González ◽  
Víctor Robles ◽  
Javier DeFelipe ◽  
...  

2013 ◽  
Vol 76 (5) ◽  
pp. 538-544 ◽  
Author(s):  
Cristina De Sousa Bolina ◽  
Regina De Sousa Bolina-Matos ◽  
Paulo Henrique De Matos Alves ◽  
Diego Pulzato Cury ◽  
Adriano Polican Ciena ◽  
...  

2004 ◽  
Vol 10 (11-12) ◽  
pp. 1684-1694 ◽  
Author(s):  
Stefan B.C. Watzka ◽  
Marianne Steiner ◽  
Puchit Samorapoompichit ◽  
Karin Gross ◽  
John G. Coles ◽  
...  

1999 ◽  
Vol 194 (1) ◽  
pp. 109-118
Author(s):  
IGNACIO DE GASPAR ◽  
MARIA JOSE BLANQUEZ ◽  
BENITO FRAILE ◽  
RICARDO PANIAGUA ◽  
MARIA ISABEL ARENAS

A histochemical, light and electron microscopy study of the hatching gland cells (HGCs) in incubated 50-d-old trout embryos is reported. The distribution of carbohydrate residues in the glycoconjugates of these cells was studied by means of a battery of 13 different lectins conjugated with horseradish peroxidase (PNA, ConA, LCA, WGA, SBA, UEA-I, HPA, DBA) or digoxigenin (DSA, MAA, AAA, SNA, GNA). Identification of N- and O-linked oligosaccharides in HGCs was performed by application of both chemical and enzymatic treatments. Present results suggest that HGCs are seromucous cells which store both high choriolytic enzyme (HCE) and low choriolytic enzyme (LCE), and that their cytoplasmic granules, endoplasmic reticulum and Golgi complex contain additional sialic acid-rich glycoproteins. The negative charge of these glycoproteins might be responsible for the rapid expansion of mucin to form a highly hydrated gel, which would facilite the action of these enzymes in programmed cell death and might play a major role during the morphogenic events.


Author(s):  
Julie A. Martini ◽  
Robert H. Doremus

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.


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