Studies on the organization and regeneration of bone marrow: Origin, growth, and differentiation of endocloned hematopoietic colonies

1983 ◽  
Vol 166 (4) ◽  
pp. 369-392 ◽  
Author(s):  
Richard H. Lambertsen ◽  
Leon Weiss
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Origin ◽  
Regeneration Of Bone

scholarly journals Some Special Aspects of Liver Repair after Resection and Administration of Multipotent Stromal Cells in Experiment

Life ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 66
Author(s):  
Igor Maiborodin ◽  
Elena Lushnikova ◽  
Marina Klinnikova ◽  
Swetlana Klochkova
Keyword(s):  
Bone Marrow ◽  
Liver Resection ◽  
Connective Tissue ◽  
Light Microscopy ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Multipotent Stromal Cells ◽  
Rapid Restoration ◽  
Bone Marrow Origin

Changes in rat liver after resection and injection of autologous multipotent mesenchymal stromal cells of bone marrow origin (MSCs) transfected with the GFP gene and cell membranes stained with red-fluorescent lipophilic membrane dye were studied by light microscopy. It was found that after the introduction of MSCs into the damaged liver, their differentiation into any cells was not found. However, under the conditions of MSCs use, the number of neutrophils in the parenchyma normalizes earlier, and necrosis and hemorrhages disappear more quickly. It was concluded that the use of MSCs at liver resection for the rapid restoration of an organ is inappropriate, since the injected cells in vivo do not differentiate either into hepatocytes, into epithelial cells of bile capillaries, into endotheliocytes and pericytes of the vascular membranes, into fibroblasts of the scar or other connective tissue structures, or into any other cells present in the liver.

scholarly journals Bone marrow-derived neural crest precursors improve nerve defect repair partially through secreted trophic factors

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Haiyan Shi ◽  
Xiaoli Li ◽  
Junling Yang ◽  
Yahong Zhao ◽  
Chengbin Xue ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Peripheral Nerve ◽  
Neural Crest ◽  
Sensory Neurons ◽  
Sensory Function ◽  
Trophic Factors ◽  
Factor Alpha ◽  
Bone Marrow Origin ◽  
Rat Bone

Abstract Background Emerging evidence suggests that neural crest-derived cells (NCCs) present important functions in peripheral nerve regeneration to correct the insufficiency of autogenous Schwann cells. Postmigratory NCCs have been successfully isolated from adult rat bone marrow in our previous work. In this study, we aim to provide neural crest-derived Schwann cell precursors (SCPs) for repair of nerve defects in adult rats, and partially reveal the mechanisms involved in neuroregeneration of cell therapy. Methods A clonal cell line of neural crest precursors of rat bone marrow origin (rBM-NCPs) with SCP identity was expanded in adherent monolayer culture to ensure the stable cell viability of NCPs and potentiate the repair of nerve defects after rBM-NCPs implantation based on tissue engineering nerve grafts (TENG). Here the behavioral, morphological, and electrophysiological detection was performed to evaluate the therapy efficacy. We further investigated the treatment with NCP-conditioned medium (NCP-CM) to sensory neurons after exposure to oxygen-glucose-deprivation (OGD) and partially compared the expression of trophic factor genes in rBM-NCPs with that in mesenchymal stem cells of bone marrow origin (rBM-MSCs). Results It was showed that the constructed TENG with rBM-NCPs loaded into silk fibroin fiber scaffolds/chitosan conduits repaired 10-mm long sciatic nerve defects more efficiently than conduits alone. The axonal regrowth, remyelination promoted the reinnervation of the denervated hind limb muscle and skin and thereby alleviated muscle atrophy and facilitated the rehabilitation of motor and sensory function. Moreover, it was demonstrated that treatment with NCP-CM could restore the cultured primary sensory neurons after OGD through trophic factors including epidermal growth factor (EGF), platelet-derived growth factor alpha (PDGFα), ciliary neurotrophic factor (CNTF), and vascular endothelial growth factor alpha (VEGFα). Conclusions In summary, our findings indicated that monolayer-cultured rBM-NCPs cell-based therapy might effectively repair peripheral nerve defects partially through secreted trophic factors, which represented the secretome of rBM-NCPs differing from that of rBM-MSCs.

scholarly journals Hyaluronic acid stimulates neovascularization during the regeneration of bone marrow after ablation

2011 ◽  
Vol 96A (3) ◽  
pp. 575-583 ◽  
Author(s):  
Andrew L. Raines ◽  
MoonHae Sunwoo ◽  
Arthur A. Gertzman ◽  
Kipling Thacker ◽  
Robert E. Guldberg ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Hyaluronic Acid ◽  
Regeneration Of Bone

scholarly journals Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector

2014 ◽  
Vol 55 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Ivana Okić-Djordjević ◽  
Drenka Trivanović ◽  
Miloš Jovanović ◽  
Marija Ignjatović ◽  
Bojana Šećerov ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Regeneration Of Bone

scholarly journals COMPARATIVE EXPERIMENTAL AND CLINICAL EVALUATION OF OSTEOGENIC PROPERTIES OF BONE MARROW AND FATTY AUTOLOGICAL STOMATIC CELLS

2018 ◽  
pp. 22-26
Author(s):  
V. F. Kutsevlyak ◽  
V. I. Kutsevlyak ◽  
I.V. Tsyganova
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adipose Tissue ◽  
Bone Tissue ◽  
Periodontal Diseases ◽  
Complex Treatment ◽  
Alveolar Process ◽  
Surgical Methods ◽  
Regeneration Of Bone Tissue ◽  
Regeneration Of Bone

The success of restorative surgical treatment of periodontal inflammatory diseases is largely determined by the processes of reparative regeneration of bone tissue of the patient. Analysis of the causes of unsuccessful treatment of such patients suggests that the ways of overcoming them consist both in improving the technology of the surgical intervention itself and in creating optimal conditions for regeneration of bone tissue. One of the most promising directions for correcting the regeneration process is the transplantation of stem cells of various origin. Using the method of directed regeneration of bone tissue of the alveolar process with the addition of autologous stem cells in periodontal surgery in the treatment of generalized periodontitis II and III degree of severity will not only eliminate the inflammatory process, but also anatomically restore the structural components of the periodontal, in particular, the alveolar bone. The purpose of the study was to compare the osteogenic properties of bone marrow and adipose tissue cells. A complex of experimental and clinical research has been carried out. In experimental studies on the effects of rabbit's autologous cells derived from bone marrow and adipose tissue, rabbits-male Chinchilla breeds of 1 year old were used to heal the pustular defects of the alveolar process; the animals were divided into 8 ordinal groups. Under thiopental anesthesia a defect of bone tissue was created at a depth of the cortical plate in the size of 0.027 cm3, which was filled as follows: a defect under the cluster; Defect and Collapan L; defect, 100000 units bone marrow stem cells, Collapan L.; defect, 500000 units Stem cells of adipose tissue, Collapan L, defect, 1 million units. CCMC, Collapan L.; defect, 100000 units SCJT, Collapan L.; defect, 500000 units SCJT, Collapan L.; defect, 1 million units. SCJT, Collapan L., after which the wound was sutured. Animals were brought out from the experiment by air embolism for 42 or 90 days. In the initial survey, the average indexes of periodontal disease in patients of both groups were equal. Radiologically, in patients of the experimental group, an increase in the height of interalveolar septal walls was determined at 1-1.5 mm, in comparison with preoperative orthopantomograms. There was an increase in the density of the spongy bone of the interdental partitions, an increase in its X-ray contrast. The use of autologous stem cells at the surgical stage of complex treatment of periodontal diseases has evident advantages over the traditional surgical methods of treating these diseases, since restoration of the periodontal structure is restored, periodontal pockets are eliminated, long remission is established, which is confirmed by the improvement of the level of oral hygiene, the normalization of the index assessment of the periodontal condition, processes of bone tissue regeneration. Thus, we have proved that the use of autologous stem cells at the surgical stage of complex treatment of periodontal diseases has evident advantages over traditional surgical methods of treatment of these diseases, since restoration of periodontal structure is restored, periodontal pockets are eliminated, long-term remission is established. Аs a result of the evaluation of the dynamics regeneration of bone defects of the mandible in case of the introduction of stem cells with Collapan into the defect zone, we found that the course was most favorable for the administration of bone tissue stem cells comparing to the introduction of adipose tissue stem cells.

Bone marrow origin of complement-receptor lymphocytes

1971 ◽  
Vol 1 (6) ◽  
pp. 491-494 ◽  
Author(s):  
P. Dukor ◽  
C. Bianco ◽  
V. Nussenzweig
Keyword(s):  
Bone Marrow ◽  
Complement Receptor ◽  
Bone Marrow Origin

scholarly journals Complex Role of the IL-4 Receptor α in a Murine Model of Airway Inflammation: Expression of the IL-4 Receptor α on Nonlymphoid Cells of Bone Marrow Origin Contributes to Severity of Inflammation

2004 ◽  
Vol 172 (7) ◽  
pp. 4545-4555 ◽  
Author(s):  
Ann E. Kelly-Welch ◽  
Marco E. F. Melo ◽  
Elizabeth Smith ◽  
Andrew Q. Ford ◽  
Christian Haudenschild ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Airway Inflammation ◽  
Murine Model ◽  
Bone Marrow Origin

Smooth-muscle progenitor cells of bone marrow origin contribute to the development of neointimal thickenings in rat aortic allografts and injured rat carotid arteries1

2002 ◽  
Vol 74 (9) ◽  
pp. 1310-1315 ◽  
Author(s):  
Piotr Religa ◽  
Krzysztof Bojakowski ◽  
Michal Maksymowicz ◽  
Maria Bojakowska ◽  
Allan Sirsj?? ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Smooth Muscle ◽  
Progenitor Cells ◽  
Bone Marrow Origin ◽  
Muscle Progenitor Cells
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