Immunocytochemical localization of epidermal growth factor during the postnatal development of the submandibular gland of the mouse

1978 ◽  
Vol 151 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Edward W. Gresik ◽  
Tibor Barka
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Postnatal Development ◽  
Submandibular Gland ◽  
Immunocytochemical Localization ◽  
Epidermal Growth

Expression of epidermal growth factor in the kidney and submandibular gland during mouse postnatal development

1990 ◽  
Vol 45 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Eduardo C. Salido ◽  
Jayaraman Lakshmanan ◽  
Larry J. Shapiro ◽  
Delbert A. Fisher ◽  
Luciano Barajas
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Postnatal Development ◽  
Submandibular Gland ◽  
Epidermal Growth

Altered secretion and processing of epidermal growth factor in adrenergic-induced growth of the rat submandibular gland

2002 ◽  
Vol 106 (1-3) ◽  
pp. 105-114 ◽  
Author(s):  
Jesper Thulesen ◽  
Mustafa Vakur Bor ◽  
Stina Thulesen ◽  
Ebba Nexø ◽  
Steen Seier Poulsen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Submandibular Gland ◽  
Epidermal Growth ◽  
Rat Submandibular Gland

Effect of pregestational sialoadenectomy of nursing mothers on eyelid opening of pups

1985 ◽  
Vol 249 (3) ◽  
pp. R285-R289
Author(s):  
S. Okamoto ◽  
T. Oka
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Submandibular Gland ◽  
Mammary Glands ◽  
Submandibular Glands ◽  
Nursing Mothers ◽  
Pregnant Mice ◽  
Epidermal Growth

The eyelid opening of pups born to and nursed by normal mice occurred by the 15th day of birth, whereas pregestational sialoadenectomy (removal of submandibular glands) of nursing mice delayed eyelid opening of their pups by as much as 5 days. Parotidectomy, however, had no effect on eyelid opening. Cross-foster nursing experiments indicated that the cause for delayed eyelid opening of pups was to be found in sialoadenectomized mothers, not their pups. Sialoadenectomized mothers had underdeveloped mammary glands that produced approximately 50% less milk than controls, and the amount of epidermal growth factor in their milk was similarly reduced. When epidermal growth factor, a polypeptide produced by the submandibular gland, was injected daily at a dose of 5 micrograms into sialoadenectomized pregnant mice, the eyelid opening of the pups nursed by their mothers occurred normally. These results are discussed with regard to the possible role of the submandibular gland and epidermal growth factor in neonatal eyelid opening.

scholarly journals Characteristics of epidermal growth factor isolated from the rat submandibular gland

1990 ◽  
Vol 52 ◽  
pp. 305
Author(s):  
Masanori Kashimata ◽  
Masahiko Hiramatsu ◽  
Makoto Murayama ◽  
Akinao Sato ◽  
Satoshi Yokose ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Submandibular Gland ◽  
Epidermal Growth ◽  
Rat Submandibular Gland

In situ localization of mRNA for epidermal growth factor in the submandibular gland of the mouse.

1985 ◽  
Vol 33 (12) ◽  
pp. 1235-1240 ◽  
Author(s):  
E W Gresik ◽  
R M Gubits ◽  
T Barka
Keyword(s):  
In Situ Hybridization ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Submandibular Gland ◽  
Cdna Probe ◽  
Coding Region ◽  
Specific Hybridization ◽  
Epidermal Growth ◽  
Convoluted Tubules

Epidermal growth factor (EGF) is a polypeptide originally isolated from the mouse submandibular gland, where it is localized immunocytochemically in cells of the granular convoluted tubules (GCT). cDNAs encoding the precursor of mouse submandibular EGF have been cloned (Scott et al. Science 221:236, 1983; Gray et al. Nature 303:722, 1983). A fragment of one of these clones, pmegf10, containing the EGF coding region, was tritium-labeled by nick-translation and used as a probe for in situ hybridization to EGF mRNA. A specific hybridization signal for EGF mRNA was seen only in mature or developing GCT cells. The intensity of the signal was stronger in glands of intact males than in females or in castrated males. In glands of castrates treated with testosterone, or of intact females treated with triiodothyronine (T3), the signal was comparable to that in intact males. In glands of males treated with T3 the intensity of the signal was stronger than in untreated males. A weak to moderate signal was seen in developing GCT cells of 20-day-old males but not females. Hybridization for 3 days gave a stronger signal than that for 1 day. No signal was seen in either sex at 10 days of age, or in control preparations exposed to labeled DNA of pBR322. The presence of EGF mRNA exclusively in GCT cells provides strong evidence that these cells are the only site of synthesis of EGF in the submandibular gland. In situ hybridization with this cDNA probe will provide a sensitive method to determine possible cellular sites of EGF production outside of the submandibular gland.

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