The fenestrated collar of mammalian cardiac sarcoplasmic reticulum: A freeze-fracture study

1977 ◽  
Vol 149 (2) ◽  
pp. 277-282 ◽  
Author(s):  
W. B. Van Winkle
Author(s):  
Paul C. Dolber ◽  
Joachim R. Sommer

A number of investigators have demonstrated by thin-section electron microscopy the presence of "coated dense vesicles" associated with the sarcoplasmic reticulum (SR) of mammalian cardiac muscle. The similarity of such vesicles to junctional SR (JSR) has been reported: They are at¬tached to SR tubules, they have "feet" at their cytoplasmic surface, and they contain electron-dense material (Fig. 1). Accordingly, and In con¬sideration of their shape, they have been named corbular SR (CSR). We here report the freeze-fracture appearance of the SR, especially the CSR, in the rabbit heart.


Author(s):  
R. A. Waugh ◽  
J. R. Sommer

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.


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