Morphology and cytochemistry of the Golgi apparatus of rat salivary gland acinar cells

1971 ◽  
Vol 130 (2) ◽  
pp. 141-157 ◽  
Author(s):  
Arthur R. Hand
Keyword(s):  
Salivary Gland ◽  
Golgi Apparatus ◽  
Acinar Cells ◽  
Rat Salivary Gland

Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells

2007 ◽  
Vol 329 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Bing Qi ◽  
Junko Fujita-Yoshigaki ◽  
Hiromi Michikawa ◽  
Keitaro Satoh ◽  
Osamu Katsumata ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Primary Cultures ◽  
Three Dimensional ◽  
Acinar Cells ◽  
Rat Salivary Gland

scholarly journals Neurofibromin expression by normal salivary glands

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Eloá Borges Luna ◽  
Pâmella Pinho Montovani ◽  
Rafaela Elvira Rozza-de-Menezes ◽  
Karin Soares Cunha
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Acinar Cells ◽  
Staining Intensity ◽  
Neurofibromatosis 1 ◽  
Tissue Type ◽  
Sublingual Gland ◽  
Minor Salivary Glands ◽  
Expression Levels ◽  
Ductal Cells

AbstractIntroductionNeurofibromin, a protein encoded by theNF1gene, is mutated in neurofibromatosis 1, one of the most common genetic diseases. Oral manifestations are common and a high prevalence of hyposalivation was recently described in individuals with neurofibromatosis 1. Although neurofibromin is ubiquitously expressed, its expression levels vary depending on the tissue type and developmental stage of the organism. The role of neurofibromin in the development, morphology, and physiology of salivary glands is unknown and a detailed expression of neurofibromin in human normal salivary glands has never been investigated.AimTo investigate the expression levels and distribution of neurofibromin in acinar and ductal cells of major and minor salivary glands of adult individuals without NF1.Material and methodTen samples of morphologically normal major and minor salivary glands (three samples of each gland: parotid, submandibular and minor salivary; and one sample of sublingual gland) from individuals without neurofibromatosis 1 were selected to assess neurofibromin expression through immunohistochemistry. Immunoquantification was performed by a digital method.ResultsNeurofibromin was expressed in the cytoplasm of both serous and mucous acinar cells, as well as in ducts from all the samples of salivary glands. Staining intensity varied from mild to strong depending on the type of salivary gland and region (acini or ducts). Ducts had higher neurofibromin expression than acinar cells (p = 0.003). There was no statistical association between the expression of neurofibromin and the type of the salivary gland, considering acini (p = 0.09) or ducts (p = 0.50) of the four salivary glands (parotid, submandibular, minor salivary, and sublingual gland). Similar results were obtained comparing the acini (p = 0.35) and ducts (p = 0.50) of minor and major salivary glands. Besides, there was no correlation between the expression of neurofibromin and age (p = 0.08), and sex (p = 0.79) of the individuals, considering simultaneously the neurofibromin levels of acini and duct (n = 34).ConclusionNeurofibromin is expressed in the cytoplasm of serous and mucous acinar cells, and ductal cells of salivary glands, suggesting that this protein is important for salivary gland function.

scholarly journals Radiation Produces Irreversible Chronic Dysfunction in the Submandibular Glands of the Rat

2012 ◽  
Vol 6 (1) ◽  
pp. 8-13 ◽  
Author(s):  
C De la Cal ◽  
J Fernández-Solari ◽  
CE Mohn ◽  
JP Prestifilippo ◽  
A Pugnaloni ◽  
...  
Keyword(s):  
Single Dose ◽  
Salivary Gland ◽  
Ionizing Radiation ◽  
Salivary Secretion ◽  
Maximal Response ◽  
Chronic Effect ◽  
Post Irradiation ◽  
Gland Function ◽  
High Doses ◽  
Rat Salivary Gland

The exposure to high doses of ionizing radiation during radiotherapy results in severe morphological and functional alterations of the salivary glands, such as xerostomia. In the present study we investigated the chronic effect of a single radiation dose of 15 Gray (Gy) limited to head and neck on rat salivary gland function (salivary secretion and gland mass) and histology. Results indicate that norepinephrine (NE)-induced salivary secretion was reduced significantly at 30, 90, 180 and 365 days after the administration of a single dose of 15 Gy of ionizing radiation compared to non-irradiated animals. The maximal secretory response was reduced by 33% at 30 and 90 days post irradiation. Interestingly, a new fall in the salivary response to NE was observed at 180 days and was maintained at 365 days post irradiation, showing a 75% reduction in the maximal response. The functional fall of the salivary secretion observed at 180 days post irradiation was not only associated with a reduction of gland mass but also to an alteration of the epithelial architecture exhibiting a changed proportion of ducts and acini, loss of eosinophilic secretor granular material, and glandular vacuolization and fibrosis. On the basis of the presented results, we conclude that ionizing radiation produces irreversible and progressive alterations of submandibular gland (SMG) function and morphology that leads to a severe salivary hypo-function.

Effects of surgical ovariectomy on rat salivary gland function

1993 ◽  
Vol 38 (9) ◽  
pp. 779-784 ◽  
Author(s):  
Karnam R. Purushotham ◽  
Pao-Li Wang ◽  
Calogero Dolce ◽  
Tivadar Zelles ◽  
Josef Blazsek ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Salivary Gland Function ◽  
Gland Function ◽  
Rat Salivary Gland

Rate of protein synthesis in rat salivary gland cells after pilocarpine or feeding

1975 ◽  
Vol 164 (4) ◽  
pp. 447-456 ◽  
Author(s):  
A. H. Kuijper-Lenstra ◽  
M. F. Kramer ◽  
W. J. van Venrooij
Keyword(s):  
Protein Synthesis ◽  
Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Rat Salivary Gland

scholarly journals DEMONSTRATION OF PEROXIDASE ACTIVITY IN TISSUE SECTIONS

1964 ◽  
Vol 12 (7) ◽  
pp. 538-544 ◽  
Author(s):  
MAX WACHSTEIN ◽  
ELIZABETH MEISEL
Keyword(s):  
Salivary Gland ◽  
Mast Cells ◽  
Guinea Pig ◽  
Peroxidase Activity ◽  
Striated Muscle ◽  
Acinar Cells ◽  
Hair Follicles ◽  
Tissue Sections ◽  
Pig Skin ◽  
Mammalian Tissues

By using an improved benzidine technique, peroxidase activity can be demonstrated in various locations in mammalian tissues. A relatively formalin resistant enzyme is found in hemoglobin and is also associated with mitochondria of striated muscle and heart. A somewhat less formalin resistant peroxidase occurs in the granules of myeloid and mast cells. A relatively formalin sensitive peroxidase is present in a number of additional locations, e.g. the acinar cells in thyroid and salivary gland, the medulla of the kidney, in hair follicles of the guinea pig skin and Kupffer cells of the liver.

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