Histochemical studies on the histogenesis of the joints in human fetuses with special reference to the development of the joint cavities in the hand and foot

1961 ◽  
Vol 108 (1) ◽  
pp. 111-122 ◽  
Author(s):  
Helge Andersen ◽  
Frede Bro-Rasmussen
2016 ◽  
Vol 49 (2) ◽  
pp. 116 ◽  
Author(s):  
Zhe Wu Jin ◽  
Yu Jin ◽  
Xiang Wu Li ◽  
Gen Murakami ◽  
José Francisco Rodríguez-Vázquez ◽  
...  

2018 ◽  
Vol 51 (3) ◽  
pp. 150 ◽  
Author(s):  
Masahito Yamamoto ◽  
Jőrg Wilting ◽  
Hiroshi Abe ◽  
Gen Murakami ◽  
Jose Francisco Rodríguez-Vázquez ◽  
...  

2017 ◽  
Vol 54 (3) ◽  
pp. 295-303 ◽  
Author(s):  
Ji Hyun Kim ◽  
Zhe Wu Jin ◽  
Shunichi Shibata ◽  
Jae Do Yang ◽  
Gen Murakami ◽  
...  

Objective To access detailed distribution and age-dependent changes of oral epithelial pearls. Design Investigation and analysis with human fetal serial sections. Setting Institute of Embryology. Methods This study examined serial frontal sections of the upper and lower jaws of 19 human fetuses at 12 to 18 weeks and of the lower jaws of four late-stage fetuses. Results The upper jaw contained more than 20 midline and more than 60 lateral pearls greater than 20 μm in diameter, whereas the lower jaw contained fewer than 30 pearls of the same size. Midline pearls in the upper jaw were often cylindrical or rugby-ball shaped, whereas all pearls in the lower jaw were small and spherical. Epithelial pearls in the upper jaw started developing along the upper midline until 12 weeks; lateral pearls and additional midline pearls (or strictly, paramedian pearls) developed until 15 weeks. In the lower jaw, however, pearl development started at 18 weeks and was almost always from the dental lamina. Some of the fetuses assessed had an open nasopalatine canal without a duct, but there was no fibrous connection between this canal and pearls. Similarly, the lip frenulum or incisive suture was not connected with these pearls. Conclusion The timing and sequence of development suggest that postfusion rupture of the palate by midline pearls was unlikely.


Author(s):  
Bruce Wetzel ◽  
Robert Buscho ◽  
Raphael Dolin

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.


Sign in / Sign up

Export Citation Format

Share Document