A study of the blood of the leopard frog, by the method of supravital staining combined with the injection of India ink into the dorsal lymph sac, with special reference to the genetic relationships among leucocytes

1925 ◽  
Vol 35 (1) ◽  
pp. 105-131 ◽  
Author(s):  
H. E. Jordan
Keyword(s):  
Special Reference ◽  
Genetic Relationships ◽  
Leopard Frog ◽  
India Ink ◽  
Supravital Staining

Genetic diversity and structure in Canadian northern leopard frog (Rana pipiens) populations: implications for reintroduction programs

2008 ◽  
Vol 86 (8) ◽  
pp. 863-874 ◽  
Author(s):  
G. A. Wilson ◽  
T. L. Fulton ◽  
K. Kendell ◽  
G. Scrimgeour ◽  
C. A. Paszkowski ◽  
...  
Keyword(s):  
Evolutionary History ◽  
Rana Pipiens ◽  
Genetic Relationships ◽  
Geographic Distance ◽  
Leopard Frog ◽  
Northern Leopard Frog ◽  
Environmental Similarity ◽  
Genetic Diversity And Structure ◽  
Source Populations ◽  
Western Portion

The northern leopard frog ( Rana pipiens Schreber, 1782) underwent a large decline in the western portion of its range and only occurs in 20% of historically occupied sites in Alberta. Its absence may reflect an inability to disperse to these sites because of habitat fragmentation, and human-mediated translocation has been proposed. In this study, we used three criteria to examine the genetic suitability of potential translocation sources: diversity, similarity to area of reintroduction, and evolutionary history. We genotyped 187 samples and sequenced 812 bp of the mitochondrial NADH dehydrogenase 1 gene from 14 Canadian northern leopard frog populations. Nuclear and mitochondrial diversity were highest in Manitoba and western Ontario and declined westward. There was no significant relationship between genetic and geographic distance, suggesting that genetic drift is a driving force affecting the genetic relationships between populations. Regions separated by more than ~50 km were quite differentiated. Therefore, source populations similar to the original inhabitants of an area for reintroduction may be uncommon. Mitochondrial analyses revealed that all populations share a close evolutionary history, belonging to the western haplotype group. While genetic criteria support the use of Manitoba and Ontario as sources, the desirability of environmental similarity to the reintroduction site suggests that ecologically exchangeable Alberta populations should also be considered.

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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