Purification of polyclonal Immunoglobulin G from human serum using peptide‐based adsorbents

AIChE Journal ◽  
2021 ◽  
Author(s):  
Wenning Chu ◽  
Sobhana A. Sripada ◽  
Hannah R. Reese ◽  
Dipendra Bhandari ◽  
Augustus Adams ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunoglobulin G ◽  
Polyclonal Immunoglobulin

scholarly journals Purification of rabbit polyclonal immunoglobulin G using anion exchangers

2011 ◽  
Vol 46 (1) ◽  
pp. 101-107 ◽  
Author(s):  
Rattana Wongchuphan ◽  
Beng Ti Tey ◽  
Wen Siang Tan ◽  
Senthil Kumar Subramanian ◽  
Farah Saleena Taip ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Anion Exchangers ◽  
Polyclonal Immunoglobulin

scholarly journals Method development of immunoglobulin G purification from micro-volumes of human serum for untargeted and targeted proteomics-based antibody repertoire studies

2019 ◽  
Vol 27 (2) ◽  
pp. 475-482
Author(s):  
Yu-Ting Chang ◽  
Ming-Chu Chang ◽  
Yun-Jung Tsai ◽  
Christine Ferng ◽  
Hsi-Chang Shih ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunoglobulin G ◽  
Method Development ◽  
Antibody Repertoire ◽  
Targeted Proteomics

Detection of inflammation/infection with human polyclonal immunoglobulin G labelled with99Tcm

1994 ◽  
Vol 67 (800) ◽  
pp. 770-774 ◽  
Author(s):  
A Jiménez-Heffernan ◽  
J L Villanueva ◽  
A Moral ◽  
A Rebollo ◽  
F M González ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Polyclonal Immunoglobulin

scholarly journals Monoclonal and polyclonal immunoglobulin G deposits on tubular basement membranes of native and pretransplant kidneys: A retrospective study

2020 ◽  
Author(s):  
Anri Sawada ◽  
Masayoshi Okumi ◽  
Shigeru Horita ◽  
Tomomi Tamura ◽  
Sekiko Taneda ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Immunoglobulin G ◽  
Basement Membranes ◽  
Polyclonal Immunoglobulin

Abstract The authors have withdrawn this preprint due to author disagreement.

Binding of enzyme--IgG complexes in human serum to Protein-A Sepharose CL-4B.

1980 ◽  
Vol 26 (2) ◽  
pp. 297-300
Author(s):  
K Bauer ◽  
P M Bayer ◽  
E Deutsch ◽  
F Gabl
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Human Serum ◽  
Immunoglobulin G ◽  
Protein A ◽  
The Other ◽  
Routine Method ◽  
Simple Method ◽  
Human Serum Protein ◽  
Enzyme Complexes

Abstract We describe a simple method for detecting enzyme--immunoglobulin G (IgG) complexes in human serum. Protein-A Sepharose CL-4B binds IgG and therefore also the enzyme--IgG complexes, which can then be separated easily from the serum by centrifugation. We demonstrate this separation in two patients, one with a complex of IgG and creatine kinase (EC 2.7.3.2) BB isoenzyme, the other with an IgG--alkaline phosphatase (EC 3.1.3.1) complex. Both patients had unexplainably high activities of the respective enzymes in their serum. The method we propose should be a useful, simple, routine method of detection in cases where IgG--enzyme complexes are suspected.

Autoantibodies to thyroxin and triiodothyronine in the immunoglobulin G fraction of serum.

1988 ◽  
Vol 34 (12) ◽  
pp. 2561-2562 ◽  
Author(s):  
L Li Calzi ◽  
S Benvenga ◽  
S Battiato ◽  
F Santini ◽  
F Trimarchi
Keyword(s):  
Thyroid Hormone ◽  
Thyroid Hormones ◽  
Human Serum ◽  
Immunoglobulin G ◽  
Subacute Thyroiditis ◽  
Total Serum ◽  
Graves Disease ◽  
Carrier Proteins ◽  
Weak Binding ◽  
Idiopathic Myxedema

Abstract Thyroid hormone antibodies (THAbs)--i.e., antibodies to thyroxin (T4) and triiodothyronine (T3)--are detected rarely in human serum, where they are searched for, possibly because of a quantitatively minimal interaction between thyroid hormones (the haptens) and serum IgGs (the antibodies). The weak binding could result from these facts: (a) there are already six physiological carrier proteins for thyroid hormones; (b) THAbs usually account for a very small fraction of the total serum IgGs; (c) THAbs may have--as reported in the literature--a relatively low affinity. To ascertain whether THAbs could pass undetected in serum, we measured antibodies to T3 and T4 in both the serum and the corresponding IgG fraction of six normal persons and 45 patients with various thyroid diseases (Graves' disease, idiopathic myxedema, Hashimoto's thyroiditis, subacute thyroiditis, tumors), using radioimmunoprecipitation. The prevalence of antibodies to T4 was 0/51 in both the sera and the IgG fractions; the prevalence of antibodies to T3 was 1/51 in both materials. Because all of the sera that tested THAb negative were confirmed to be so in the THAb assay of the IgG fraction, we conclude that the prevalence of serum THAbs is not underestimated and that autoimmunization against thyroid hormones is really a rare phenomenon.

Polyclonal immunoglobulin G deposition on the tubular basement membrane in a diabetic nephropathy: A case report

2020 ◽  
Vol 70 (7) ◽  
pp. 463-469
Author(s):  
Hirofumi Watanabe ◽  
Yoichi Takeuchi ◽  
Shinji Taniuchi ◽  
Hiroshi Sato ◽  
Yasuhiro Nakamura ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Case Report ◽  
Basement Membrane ◽  
Immunoglobulin G ◽  
Tubular Basement Membrane ◽  
Polyclonal Immunoglobulin

Possible role of polyclonal Immunoglobulin G (IgG) of patients with different clinical manifestations of the antiphospholipid syndrome on activation of inflammation biomarkers

Placenta ◽  
2019 ◽  
Vol 83 ◽  
pp. e86-e87
Author(s):  
Carlos Mario Rodríguez Colorado ◽  
Manuela Velasquez Berrío ◽  
Diana Carolina Rúa Molina ◽  
Angela María Álvarez Gomez ◽  
Angela Patricia Cadavid Jaramillo
Keyword(s):  
Antiphospholipid Syndrome ◽  
Immunoglobulin G ◽  
Clinical Manifestations ◽  
Polyclonal Immunoglobulin
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