scholarly journals Comprehensive mutagenesis on yeast cytosine deaminase yields improvements in 5‐fluorocytosine toxicity in HT1080 cells

AIChE Journal ◽  
2019 ◽  
Vol 66 (3) ◽  
Author(s):  
Tiana D. Warren ◽  
Krishna Patel ◽  
Jordan L. Rivera ◽  
James R. Eshleman ◽  
Marc Ostermeier
Keyword(s):  
Cytosine Deaminase ◽  
Ht1080 Cells ◽  
Yeast Cytosine Deaminase

Protein-Programmed Accumulation of Yeast Cytosine Deaminase in Cancer Cells in Response to Mock-Hypoxia

2019 ◽  
Vol 8 (5) ◽  
pp. 948-954 ◽  
Author(s):  
Tiana D. Warren ◽  
Krishna Patel ◽  
James R. Eshleman ◽  
Marc Ostermeier
Keyword(s):  
Cancer Cells ◽  
Cytosine Deaminase ◽  
Yeast Cytosine Deaminase

Bystander cytotoxicity in human medullary thyroid carcinoma cells mediated by fusion yeast cytosine deaminase and 5-fluorocytosine

2011 ◽  
Vol 311 (1) ◽  
pp. 101-112 ◽  
Author(s):  
Lucia Kucerova ◽  
Miroslava Matuskova ◽  
Kristina Hlubinova ◽  
Roman Bohovic ◽  
Lucia Feketeova ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Medullary Thyroid Carcinoma ◽  
Cytosine Deaminase ◽  
Carcinoma Cells ◽  
Medullary Thyroid ◽  
Yeast Cytosine Deaminase

scholarly journals A Molecular Dynamics Study of the Ligand Release Path in Yeast Cytosine Deaminase

2007 ◽  
Vol 92 (7) ◽  
pp. 2301-2310 ◽  
Author(s):  
Lishan Yao ◽  
Honggao Yan ◽  
Robert I. Cukier
Keyword(s):  
Molecular Dynamics ◽  
Cytosine Deaminase ◽  
Yeast Cytosine Deaminase

scholarly journals Role of Glutamate 64 in the Activation of the Prodrug 5-Fluorocytosine by Yeast Cytosine Deaminase

Biochemistry ◽  
2011 ◽  
Vol 51 (1) ◽  
pp. 475-486 ◽  
Author(s):  
Jifeng Wang ◽  
Stepan Sklenak ◽  
Aizhuo Liu ◽  
Krzysztof Felczak ◽  
Yan Wu ◽  
...  
Keyword(s):  
Cytosine Deaminase ◽  
Yeast Cytosine Deaminase

Product Release Is Rate-Limiting in the Activation of the Prodrug 5-Fluorocytosine by Yeast Cytosine Deaminase†

Biochemistry ◽  
2005 ◽  
Vol 44 (15) ◽  
pp. 5940-5947 ◽  
Author(s):  
Lishan Yao ◽  
Yue Li ◽  
Yan Wu ◽  
Aizhuo Liu ◽  
Honggao Yan
Keyword(s):  
Cytosine Deaminase ◽  
Product Release ◽  
Rate Limiting ◽  
Yeast Cytosine Deaminase

scholarly journals Crystal Structure of tRNA Adenosine Deaminase (TadA) fromAquifex aeolicus

2005 ◽  
Vol 280 (16) ◽  
pp. 16002-16008 ◽  
Author(s):  
Mitsuo Kuratani ◽  
Ryohei Ishii ◽  
Yoshitaka Bessho ◽  
Ryuya Fukunaga ◽  
Toru Sengoku ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Adenosine Deaminase ◽  
Cytosine Deaminase ◽  
Amino Acid Residues ◽  
Aquifex Aeolicus ◽  
Stem Loop ◽  
Dimeric Structure ◽  
Docking Model ◽  
Tetrameric Structure ◽  
Yeast Cytosine Deaminase

The bacterial tRNA adenosine deaminase (TadA) generates inosine by deaminating the adenosine residue at the wobble position of tRNAArg-2. This modification is essential for the decoding system. In this study, we determined the crystal structure ofAquifex aeolicusTadA at a 1.8-Å resolution. This is the first structure of a deaminase acting on tRNA.A. aeolicusTadA has an α/β/α three-layered fold and forms a homodimer. TheA. aeolicusTadA dimeric structure is completely different from the tetrameric structure of yeast CDD1, which deaminates mRNA and cytidine, but is similar to the dimeric structure of yeast cytosine deaminase. However, in theA. aeolicusTadA structure, the shapes of the C-terminal helix and the regions between the β4 and β5 strands are quite distinct from those of yeast cytosine deaminase and a large cavity is produced. This cavity contains many conserved amino acid residues that are likely to be involved in either catalysis or tRNA binding. We made a docking model of TadA with the tRNA anticodon stem loop.

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