scholarly journals Effects of 3-D microwell culture on initial fate specification in human embryonic stem cells

AIChE Journal ◽  
2014 ◽  
Vol 60 (4) ◽  
pp. 1225-1235 ◽  
Author(s):  
Cheston Hsiao ◽  
Matthew Tomai ◽  
Jeremy Glynn ◽  
Sean P. Palecek
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Fate Specification

scholarly journals E2A regulates neural ectoderm fate specification in human embryonic stem cells

Development ◽  
2020 ◽  
Vol 147 (23) ◽  
pp. dev190298
Author(s):  
Siqi Yi ◽  
Xiaotian Huang ◽  
Shixin Zhou ◽  
Yuan Zhou ◽  
Michele K. Anderson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Fate ◽  
Human Embryonic Stem Cells ◽  
Gene Locus ◽  
Embryonic Stem ◽  
Cell Fate Decisions ◽  
Fate Specification ◽  
E Protein ◽  
Neural Ectoderm

ABSTRACTE protein transcription factors are crucial for many cell fate decisions. However, the roles of E proteins in the germ-layer specification of human embryonic stem cells (hESCs) are poorly understood. We disrupted the TCF3 gene locus to delete the E protein E2A in hESCs. E2A knockout (KO) hESCs retained key features of pluripotency, but displayed decreased neural ectoderm coupled with enhanced mesoendoderm outcomes. Genome-wide analyses showed that E2A directly regulates neural ectoderm and Nodal pathway genes. Accordingly, inhibition of Nodal or E2A overexpression partially rescued the neural ectoderm defect in E2A KO hESCs. Loss of E2A had little impact on the epigenetic landscape of hESCs, whereas E2A KO neural precursors displayed increased accessibility of the gene locus encoding the Nodal agonist CRIPTO. Double-deletion of both E2A and HEB (TCF12) resulted in a more severe neural ectoderm defect. Therefore, this study reveals critical context-dependent functions for E2A in human neural ectoderm fate specification.

Ethanol alters cell cycle gene expression in human embryonic stem cells

2016 ◽  
Vol 01 (03) ◽  
pp. 201-208 ◽  
Author(s):  
Malini Krishnamoorthy ◽  
Brian Gerwe ◽  
Jamie Heimburg-Molinaro ◽  
Rachel Nash ◽  
Jagan Arumugham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Cycle Gene ◽  
Cell Cycle Gene Expression
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