Agronomic characterization of cotton genotypes susceptible and resistant to Rotylenchulus reniformis in the US Midsouth

2021 ◽  
Author(s):  
Bhupinder Singh ◽  
Daryl Chastain ◽  
K. Raja Reddy ◽  
John Snider ◽  
L. Jason Krutz ◽  
...  
2021 ◽  
Vol 85 (3) ◽  
pp. AB47
Author(s):  
William Murphy ◽  
Vartan Pahalyants ◽  
Nicole Gunasekera ◽  
Connie Shi ◽  
Vinod Nambudiri

Author(s):  
Richard Olson ◽  
Paul Scott

The US NRC/EPRI xLPR (eXtremely Low Probability of Rupture) probabilistic pipe fracture analysis program uses deterministic modules as the foundation for the calculation of the probability of pipe leak or rupture as a consequence of active degradation mechanisms, vibration or seismic loading. The circumferential crack opening displacement module, CrCOD, estimates crack opening displacement (COD) at the inside pipe surface, at the mid-wall thickness location, and at the outside pipe surface using a combined tension/crack face pressure/bending GE/EPRI-like solution. Each module has an uncertainty beyond the uncertainty of the xLPR data inputs. This paper documents the uncertainty for CrCOD. Using 36 pipe fracture experiments, including: base metal, similar metal weld, and dissimilar metal weld experiments; bend only and pressure and bend loading; static and dynamic load histories; cracks that range from short to long, the uncertainty of the CrCOD methodology is characterized. Module uncertainty is presented in terms mean fit and standard deviation between prediction and experimental values.


2021 ◽  
Vol 15 (1) ◽  
pp. 129-138
Author(s):  
Raegan S. Hoefler ◽  
Indira T. Kudva

Background: Differences between Escherichia coli O157 (O157) strains are well-established with some of these strains being associated with major outbreaks in the US. EDL933 is one such O157 strain that caused a multistate outbreak in 1982 and has since been used as a prototype in various O157-related experiments. Objective: As O157 can readily acquire genetic mutations, we sought to determine if the genetic and phenotypic profiles of EDL933 strains from different sources would be consistent. Methods: We evaluated wild-type O157 strains stocked as EDL933 from three different laboratories, in the strain typing Polymorphic Amplified Typing Sequence (PATS) and the bovine rectal-anal junction squamous epithelial (RSE) cell- and HEp-2 cell- adherence assays. In addition, we also verified if Shiga toxins (Stx), the Locus of Enterocyte Effacement (LEE) or curli fimbriae contributed to the adherence phenotypes observed using mutant and wild-type EDL933 isolates. Results: Our results showed differences in PATS profiles and RSE cell-adherence phenotype, with no influence from the Stx or LEE genes, between EDL933 from different sources. Interestingly, the EDL933 strain that demonstrated the most contrasting diffuse adherence phenotype on RSE cells, EDL933-T, had decreased curli production that may have contributed to this phenotype. Conclusion: Our observations suggest that a comprehensive characterization of bacterial isolates, even if assigned to the same strain type prior to use in experiments, is warranted to ensure consistency and reproducibility of results.


2003 ◽  
Vol 206 (4-5) ◽  
pp. 437-445 ◽  
Author(s):  
Rajendra S. Chhabra ◽  
John R. Bucher ◽  
Mary Wolfe ◽  
Christopher Portier

2013 ◽  
Vol 85 (2) ◽  
pp. 283-292 ◽  
Author(s):  
Tara A. Edwards ◽  
Nicole A. Calica ◽  
Dolores A. Huang ◽  
Namritha Manoharan ◽  
Weiguo Hou ◽  
...  

2014 ◽  
Vol 2 ◽  
Author(s):  
Carolyn A. Young ◽  
Nikki D. Charlton ◽  
Johanna E. Takach ◽  
Ginger A. Swoboda ◽  
Michael A. Trammell ◽  
...  
Keyword(s):  

2012 ◽  
Vol 142 (5) ◽  
pp. S-965
Author(s):  
Salil D. Sheth ◽  
Montserrat Vera-Llonch ◽  
Joseph P. Lynch ◽  
Winifred Werther ◽  
Raymond Rubin
Keyword(s):  

2016 ◽  
Vol 37 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Jared P. Wood ◽  
Todd S. Campbell ◽  
Robert B. Page

We used 454 pyrosequencing to characterize 17 novel microsatellite loci from the Nile monitor (Varanus niloticus), using samples from an invasive population in the US state of Florida. These markers were screened for variation in 40 individuals from the City of Cape Coral. Observed heterozygosity ranged from 0.18 to 0.73 (mean ± SE = 0.46 ± 0.06) and the number of alleles per locus ranged from 1 to 4 (mean ± SE = 2.5 ± 0.2). Preliminary genotyping of samples from populations in West Palm Beach and Homestead revealed two loci (Mon6andMon15) that are monomorphic in Cape Coral, but polymorphic in these other two populations. We found no statistical departures from Hardy-Weinberg equilibrium or evidence of null alleles. However, there was statistical evidence for genotypic disequilibrium betweenMon1-Mon14andMon3-Mon8. BLASTn searches of NCBI’s nr/nt database identified four microsatellites containing 454 fragments that exhibit substantial sequence similarity to loci in other reptilian genomes, suggesting these markers should be prioritized by researchers interested in cross-amplification in other varanids. The resources we have developed will enable investigations of the population genetic dynamics of invasive Nile monitors in Florida and may facilitate studies within this species native range.


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