scholarly journals Identification microRNAs and target genes in Tibetan hulless barley to BLS infection

2021 ◽  
Author(s):  
Xiaohua Yao ◽  
Yue Wang ◽  
Youhua Yao ◽  
Yixiong Bai ◽  
Kunlun Wu ◽  
...  
Keyword(s):  
Target Genes ◽  
Hulless Barley ◽  
Tibetan Hulless Barley

scholarly journals Identification and Profiling of microRNAs and Their Target Genes in Tibetan Hulless Barley in Response to Barley Leaf Stripe (BLS) Infection

2020 ◽  
Author(s):  
Xiaohua Yao ◽  
Yue Wang ◽  
Youhua Yao ◽  
Yixiong Bai ◽  
Kunlun Wu ◽  
...  
Keyword(s):  
Target Genes ◽  
Tibet Plateau ◽  
Sequencing Analysis ◽  
Hulless Barley ◽  
Leaf Stripe ◽  
Before And After ◽  
Candidate Target ◽  
Barley Leaf ◽  
Tibetan Hulless Barley ◽  
Barley Leaf Stripe

Abstract Background Tibetan hulless barley is widely grown on the Qinghai-Tibet Plateau, where it has served as a staple food for Tibetan people since the 5th century CE. Barley leaf stripe (BLS) is one of the most severe fungal diseases affecting the yield and quality of Tibetan hulless barley. Results Here, we compared the miRNA profiles before and after BLS in two Tibetan hulless barley genotypes: Z1141, a BLS-sensitive wild variety, and Kunlun14, a BLS-tolerant hybrid variety. A total of 36 conserved and 56 novel miRNAs were identified. Of these, 10 conserved and 10 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Kunlun14, respectively, while 3 conserved and 5 novel miRNAs exhibited significantly changed expression between the normal and infected leaves of Z1141, respectively. A total of 24 miRNAs were found in Z1141 and Kunlun14, and a further 546 putative target genes were predicted. Transcriptome sequencing analysis showed that among the 546 candidate genes, 131 had significant differences in expression between the normal and infected leaves of Kunlun14 and Z1141. Gene ontology, pathway, and Blast analyses indicated 10 candidate target genes that were involved in the barley disease resistance. These 10 candidate target genes may be regulated by 7 miRNAs. According to quantitative real-time PCR results, the 10 targets were negatively correlated with their corresponding miRNAs after infection with BLS. Conclusions The miRNAs and their target genes expressed in Tibetan hulless barley were identified and found to be associated with BLS resistance. Thus, these miRNAs and their target genes may be exploited via breeding programs or genetic engineering to improve BLS resistance in Tibetan hulless barley.

scholarly journals Qualitative and semi-quantitative assessment of anthocyanins in Tibetan hulless barley from different geographical locations by UPLC-QTOF-MS and their antioxidant capacities

2021 ◽  
Vol 19 (1) ◽  
pp. 1-8
Author(s):  
Tangwei Zhang ◽  
Jialin Ma ◽  
Xuelian Wu ◽  
Zhihua Hao ◽  
Ci Dun ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Quantitative Assessment ◽  
Ultra Performance Liquid Chromatography ◽  
Total Anthocyanin ◽  
Hulless Barley ◽  
Flight Mass Spectrometry ◽  
Antioxidant Capacities ◽  
Tibetan Hulless Barley ◽  
Geographical Locations

Abstract Tibetan hulless barley (“qingke” in Chinese) is a valuable food in Tibet. Purple qingke (PQK) and black qingke (BQK), two special pigmented types of hulless barley, have traditionally been widely cultivated and consumed in Tibet for thousands of years. The composition and contents of anthocyanins of two cultivars are unknown. This study aimed to explore the composition and contents of anthocyanins of two cultivars and their antioxidant capacities. Six anthocyanins were identified by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry from barley, most of the anthocyanins were acylated by malonyl group. The total anthocyanin contents ranged from 141 to 2,304 μg/g in PQK and from 248.7 to 2902.9 μg/g in BQK. Furthermore, qingke has strong antioxidant activity against DPPH, ABTS˙+, and FRAP. Qingke may be useful for treating or preventing diseases caused by the overproduction of radicals.

Salicylic Acid Decreases the Levels of Dehydrin-Like Proteins in Tibetan Hulless Barley Leaves under Water Stress

2006 ◽  
Vol 61 (3-4) ◽  
pp. 245-250 ◽  
Author(s):  
Xin Sun ◽  
Shu Yuan ◽  
Hong-Hui Lin
Keyword(s):  
Salicylic Acid ◽  
Water Stress ◽  
Electrolyte Leakage ◽  
Oxidative Injury ◽  
Hulless Barley ◽  
Barley Leaves ◽  
Tibetan Hulless Barley

The effects of salicylic acid (SA) on the accumulation of dehydrins in leaves of Tibetan hulless barley seedlings under water stress were investigated. The results indicated that SA decreased the levels of the four dehydrin-like proteins induced by water stress. The concentrations of these dehydrin-like proteins increased under water stress. However, their levels in SA-pretreated seedlings were always lower than in those receiving only water stress. Our results also indicated that the levels of dehydrin-like proteins decreased as the SA concentration increased. In SA-pretreated seedlings, electrolyte leakage, MDA and H2O2 content were rather higher than in seedlings receiving only water stress. By these results, we suggest that lower levels of dehydrin-like proteins in seedlings with SA treatment may be due to the greater accumulation of H2O2 induced by SA, which causes more oxidative injury under water stress.

The complete chloroplast genome of Tibetan hulless barley

2015 ◽  
Vol 28 (3) ◽  
pp. 324-325 ◽  
Author(s):  
Quan Xing Zeng ◽  
Jun Hong Yuan ◽  
Lin Yu Wang ◽  
Jun Qi Xu ◽  
Tashi Nyima
Keyword(s):  
Chloroplast Genome ◽  
Complete Chloroplast Genome ◽  
Hulless Barley ◽  
Tibetan Hulless Barley

scholarly journals Transcriptome analysis revealed the drought-responsive genes in Tibetan hulless barley

BMC Genomics ◽  
2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Xingquan Zeng ◽  
Lijun Bai ◽  
Zexiu Wei ◽  
Hongjun Yuan ◽  
Yulin Wang ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Hulless Barley ◽  
Tibetan Hulless Barley

scholarly journals Purification, Preliminary Structural Characterization, and In Vitro Inhibitory Effect on Digestive Enzymes by β-Glucan from Qingke (Tibetan Hulless Barley)

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Jialiang Hu ◽  
Yue Wu ◽  
Huifang Xie ◽  
Wanyin Shi ◽  
Zhiyuan Chen ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Structural Characterization ◽  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Hordeum Vulgare L ◽  
Spectra Analysis ◽  
Hulless Barley ◽  
Ft Ir ◽  
Tibetan Hulless Barley

Background and Objective. Qingke (Tibetan hulless barley, Hordeum vulgare L.) contains a high content of β-glucan among all the cereal varieties. Although β-glucan has multiple physiological functions, the physiological function of qingke β-glucan was few studied. In this study, the β-glucan was isolated, purified, determined the structural characterization, and measured the inhibitory activity to enzymes correlating blood sugar and lipid. Methods. β-Glucan was isolated from enzymatic aqueous extract of qingke by using deproteinization, decolorization, DEAE-52 column chromatography, and sepharose CL-4B agarose gel column chromatography. The structure of the β-glucan was determined using FT-IR and 13C-NMR spectra analysis, and molecular mass by use of HPSEC-dRI-LS. The kinematic viscosity was measured. The inhibitory effects of this β-glucan on four enzymes were investigated. Results. This β-glucan had a uniform molecular weight of 201,000 Da with β-(1⟶4) as the main chain and β-(1⟶3) as a side chain. The β-glucan presented a relatively strong inhibitory activity on α-glucosidase, moderate inhibition on invertase, and a weak inhibition on α-amylase, whereas it did not inhibit lipase. Conclusion. The study indicates that the enzymatic β-glucan from qingke has the potential as natural auxiliary hypoglycemic additives in functional medicine or foods.

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