scholarly journals Cell‐Free Protein Synthesis: A Cell‐Free Platform Based on Nisin Biosynthesis for Discovering Novel Lanthipeptides and Guiding their Overproduction In Vivo (Adv. Sci. 17/2020)

2020 ◽  
Vol 7 (17) ◽  
pp. 2070096
Author(s):  
Ran Liu ◽  
Yuchen Zhang ◽  
Guoqing Zhai ◽  
Shuai Fu ◽  
Yao Xia ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
A Cell ◽  
Cell Free Protein Synthesis

scholarly journals A User’s Guide to Cell-Free Protein Synthesis

2019 ◽  
Vol 2 (1) ◽  
pp. 24 ◽  
Author(s):  
Nicole E. Gregorio ◽  
Max Z. Levine ◽  
Javin P. Oza
Keyword(s):  
Protein Synthesis ◽  
Protein Expression ◽  
Living Cells ◽  
Development Education ◽  
Free Protein ◽  
Therapeutic Development ◽  
A Cell ◽  
New Applications ◽  
Cell Free Protein Synthesis

Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on living cells, and the ability to focus all system energy on production of the protein of interest. Over the last 60 years, the CFPS platform has grown and diversified greatly, and it continues to evolve today. Both new applications and new types of extracts based on a variety of organisms are current areas of development. However, new users interested in CFPS may find it challenging to implement a cell-free platform in their laboratory due to the technical and functional considerations involved in choosing and executing a platform that best suits their needs. Here we hope to reduce this barrier to implementing CFPS by clarifying the similarities and differences amongst cell-free platforms, highlighting the various applications that have been accomplished in each of them, and detailing the main methodological and instrumental requirement for their preparation. Additionally, this review will help to contextualize the landscape of work that has been done using CFPS and showcase the diversity of applications that it enables.

Translational incorporation of multiple unnatural amino acids in a cell-free protein synthesis system

2014 ◽  
Vol 19 (3) ◽  
pp. 426-432 ◽  
Author(s):  
Su-Jin Oh ◽  
Kyung-Ho Lee ◽  
Ho-Cheol Kim ◽  
Christy Catherine ◽  
Hyungdon Yun ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Unnatural Amino Acids ◽  
Synthesis System ◽  
Free Protein ◽  
A Cell ◽  
Protein Synthesis System ◽  
Cell Free Protein Synthesis

Temperature effect on a cell free protein synthesis of rat testis

FEBS Letters ◽  
1980 ◽  
Vol 112 (2) ◽  
pp. 221-224 ◽  
Author(s):  
Masahisa Nakamura ◽  
Peter F. Hall ◽  
Junzo Kato
Keyword(s):  
Protein Synthesis ◽  
Temperature Effect ◽  
Free Protein ◽  
Rat Testis ◽  
A Cell ◽  
Cell Free Protein Synthesis

Effect of in vivo administration of somatostatin on cell-free protein synthesis of pituitary and of adrenal ribosomes

Metabolism ◽  
1978 ◽  
Vol 27 (9) ◽  
pp. 1387-1390 ◽  
Author(s):  
Karl-Heinz Tragl ◽  
Helmut Kinast ◽  
Helmut Steininger ◽  
Georg Geyer
Keyword(s):  
Protein Synthesis ◽  
Free Protein ◽  
In Vivo Administration ◽  
Cell Free Protein Synthesis
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