scholarly journals Conduction Cooling and Plasmonic Heating Dramatically Increase Droplet Vitrification Volumes for Cell Cryopreservation

2021 ◽  
pp. 2004605
Author(s):  
Li Zhan ◽  
Shuang‐Zhuang Guo ◽  
Joseph Kangas ◽  
Qi Shao ◽  
Maple Shiao ◽  
...  
1982 ◽  
Vol 26 (1) ◽  
pp. 45-54 ◽  
Author(s):  
R. C. Chu ◽  
U. P. Hwang ◽  
R. E. Simons

2013 ◽  
Vol 41 (2) ◽  
pp. 638 ◽  
Author(s):  
Aylin OZUDOGRU ◽  
Diogo Pedrosa Corrêa Da SILVA ◽  
Ergun KAYA ◽  
Giuliano DRADI ◽  
Renato PAIVA ◽  
...  

The study focused on an economically-important ornamental outdoor shrub, Nandina domestica, with the aims to (i) optimize an effective in vitro conservation method, and (ii) develop a cryopreservation protocol for shoot tips by the PVS2 vitrification and droplet-vitrification techniques. For in vitro conservation of shoot cultures, the tested parameters were sucrose content in the storage medium (30, 45, 60 g/L) and storage temperature (4 °C or 8 °C). Cryopreservation was performed by applying the PVS2 vitrification solution, in 2-ml cryovials or in drops over aluminum foil strips, for 15, 30, 60 or 90 min at 0 °C, followed by the direct immersion in liquid nitrogen of shoot tips. Results show that N. domestica shoots can be conserved successfully for 6 months at both the temperatures tested, especially when 60 g/L sucrose is used in the storage medium. However, conservation at 4 °C showed to be more appropriate, as hyperhydricity was observed in post-conservation of shoots coming from storage at 8 °C. As for cryopreservation, a daily gradual increase of sucrose concentration (from 0.25 to 1.0 M) produced better protection to the samples that were stored in liquid nitrogen. Indeed, with this sucrose treatment method, a 30-min PVS2 incubation time was enough to produce, 60 days after thawing, the best recovery (47% and 50%) of shoot tips, cryopreserved with PVS2 vitrification and droplet-vitrification, respectively.


2017 ◽  
Vol 871 ◽  
pp. 012089
Author(s):  
Van Quan Dao ◽  
Taekue Kim ◽  
Thang Le Tat ◽  
Haejin Sung ◽  
Jongho Choi ◽  
...  

RSC Advances ◽  
2017 ◽  
Vol 7 (64) ◽  
pp. 40218-40226 ◽  
Author(s):  
Mahesh K. Gangishetty ◽  
Adriana M. Fontes ◽  
Marcos Malta ◽  
Timothy L. Kelly ◽  
Robert W. J. Scott

Au@Pd nanotriangles are used to accelerate coupling and hydrogenation reactions by a plasmonic heating mechanism.


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