Bio‐Inspired Multi‐Mode Pain‐Perceptual System (MMPPS) with Noxious Stimuli Warning, Damage Localization, and Enhanced Damage Protection

Advanced Science ◽

10.1002/advs.202004208 ◽

2021 ◽

pp. 2004208

Author(s):

Fali Li ◽

Shuang Gao ◽

Ying Lu ◽

Waqas Asghar ◽

Jinwei Cao ◽

...

Keyword(s):

Perceptual System ◽

Damage Localization ◽

Noxious Stimuli ◽

Multi Mode ◽

Damage Protection

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Multi‐Mode Pain‐Perceptual System: Bio‐Inspired Multi‐Mode Pain‐Perceptual System (MMPPS) with Noxious Stimuli Warning, Damage Localization, and Enhanced Damage Protection (Adv. Sci. 10/2021)

Advanced Science ◽

10.1002/advs.202170055 ◽

2021 ◽

Vol 8 (10) ◽

pp. 2170055

Author(s):

Fali Li ◽

Shuang Gao ◽

Ying Lu ◽

Waqas Asghar ◽

Jinwei Cao ◽

...

Keyword(s):

Perceptual System ◽

Damage Localization ◽

Noxious Stimuli ◽

Multi Mode ◽

Damage Protection

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Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100166117 ◽

1996 ◽

Vol 54 ◽

pp. 730-731

Author(s):

E. D. Salmon ◽

J. C. Waters ◽

C. Waterman-Storer

Keyword(s):

Imaging System ◽

Ccd Camera ◽

Transmitted Light ◽

Microtubule Assembly ◽

Live Cells ◽

Optical Efficiency ◽

Multiple Band ◽

Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

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