scholarly journals Silver Covalently Bound to Cyanographene Overcomes Bacterial Resistance to Silver Nanoparticles and Antibiotics

2021 ◽  
pp. 2003090
Author(s):  
David Panáček ◽  
Lucie Hochvaldová ◽  
Aristides Bakandritsos ◽  
Tomáš Malina ◽  
Michal Langer ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Resistance ◽  
Covalently Bound

scholarly journals Microbial Resistance: Silver Covalently Bound to Cyanographene Overcomes Bacterial Resistance to Silver Nanoparticles and Antibiotics (Adv. Sci. 12/2021)

2021 ◽  
Vol 8 (12) ◽  
pp. 2170065
Author(s):  
David Panáček ◽  
Lucie Hochvaldová ◽  
Aristides Bakandritsos ◽  
Tomáš Malina ◽  
Michal Langer ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Resistance ◽  
Microbial Resistance ◽  
Covalently Bound

Temperature-sensitive poly(N-isopropylacrylamide) (PNIPAAm) stabilized size controllable synthesis of silver nanoparticles and its improved antimicrobial activity for wound healing and nursing care after femoral fracture during surgery

2021 ◽  
Vol 11 (1) ◽  
pp. 73-84
Author(s):  
Xiaoyun Wang ◽  
Jing Zhang
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Femoral Fracture ◽  
Bacterial Resistance ◽  
Polymer Nanocomposite ◽  
Temperature Sensitive ◽  
Solution Stability ◽  
Uniform Dispersion ◽  
Significant Temperature ◽  
Thermosensitive Copolymer

In this research, a thermo-sensitive copolymer PNIPAAm-co-MHq was used to successfully synthesize different nano sizes silver nanoparticles in the ranges between 1.5 to 4 nm with uniform dispersion. The polymeric assisted synthesized Ag nanoparticles (AgNPs@PM) exhibited reasonable solution stability and thermalresponsive behaviour. In specific, AgNPs@PM3 (1.59 nm) displayed improved bacterial resistance against clinically approved anti-biotic resistant bacterial pathigens with very low MIC value (4.05 μg/mL). Subsequently, the thermal responsive polymeric molecular structure on AgNPs synthesis has been established that significant temperature dependened anti-bacterial efficiency. It was also observed that the nonparticipants size, temperature responses and proportion of thermosensitive copolymer also influenced the antibacterial efficacy of AgNPs@PM. Resulting thermal sensitive polymer nanocomposite can be extremely beneficial for wound healing treatment after femoral fracture surgery.

scholarly journals Antibacterial Effect of Silver Nanoparticles on Klebsiella spp

2020 ◽  
Vol 1 (2) ◽  
pp. 8-15
Author(s):  
Gislanne Stéphanne Estevam da Silva ◽  
Rivaldo Leon Bezerra Cabral ◽  
Nathalie de Sena Pereira ◽  
José Heriberto Oliveira do Nascimento ◽  
Dany G kramer
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Resistance ◽  
Brain Heart Infusion ◽  
Trisodium Citrate ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
Negative Control ◽  
In Vitro Tests ◽  
Klebsiella Spp

Silver nanoparticles (AgNP) can be incorporated into medical devices, such as tissues, to circumvent bacterial resistance such as Klebsiella spp, which can lead to skin and mucosal infections. Thus, the aim of the present study was to synthesize silver nanoparticles for later incorporation into cotton fabrics and in vitro tests against Klebsiella spp. The AgNP colloidal solution was synthesized (AgNO3 - 0.1 mM, 100 mM trisodium citrate, polyvinylpyrrolidone - 0.24 g, H2OH2) and then impregnated into the cotton fabric pretreated with poly diallyl dimethylammonium chloride (PDDA) of 100/500 tissue, shaken for 30 minutes). The material produced was analyzed by the FTIR; DLS and reflectance spectroscopy. The tests of the antimicrobial activities were by the microdilution technique against Klebsiella spp, in tubes containing Brain Heart Infusion (BHI), with the solution of silver (1); Tissue containing AgNP - 4 mm (2); Negative control (3) and positive control - ceftriaxone (4). Regarding MIC, the inhibitory activity occurred of the dilutions between 1/2 and 1/16. The AgNP particles had an average size of 24.75 nm. As synthesized AgNPs demonstrate the excellent antimicrobial activity against Klebsiella spp, with special emphasis on applications in nanotechnology and nanomedicine, targeting multiresistant antibiotic bacteria.

scholarly journals Characterization of Silver Nanoparticles Obtained by a Green Route and Their Evaluation in the Bacterium of Pseudomonas aeruginosa

Crystals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 395 ◽  
Author(s):  
Juan Carlos Martínez Espinosa ◽  
Raúl Carrera Cerritos ◽  
Maria Antonieta Ramírez Morales ◽  
Karla Paola Sánchez Guerrero ◽  
Rocio Alejandra Silva Contreras ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
Bacterial Resistance ◽  
Antimicrobial Effect ◽  
X Ray ◽  
Transmission Electron ◽  
Green Route

Metal nanoparticles are widely used in different areas such as biotechnology and biomedicine, for example in drug delivery, imaging and control of bacterial growth. The antimicrobial effect of silver has been identified as an alternative approach to the increasing bacterial resistance to antibiotics. Silver nanoparticles were synthesized by the green route using the Geranium extract as a reducing agent. The characterization was carried out by the techniques of UV-Vis spectrophotometry, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), X-ray emitted photoelectron spectroscopy (XPS) and X-ray diffraction. Nanoparticle diameters between 15 and 50 nm were obtained and the interplanar spaces calculated from the electron diffraction pattern corresponding to a mixture of silver with 4H and FCC structures. To determine the minimum inhibitory concentration of silver nanoparticles (AgNPs) on the Pseudomonas aeruginosa bacteria (ATCC-27853), different concentrations of colloidal solution 0.36, 0.18, 0.09 and 0.05 μg/mL were evaluated as a function of the incubation time, measuring the inhibition halo and colony forming unit (CFU) during 0, 2 and 4 h of incubation. The minimum inhibitory AgNPs concentration (MIC) is 0.36 μg/mL at 0 h while the concentration of 0.18 μg/mL presents a total inhibition of the bacterium after 2 h. For the rest of the dilutions, gradual inhibitions as a function of time were observed. We evaluate the antibacterial effect of silver nanoparticles obtained by a green methodology in Pseudomonas aeruginosa bacteria. Finally, the colloidal nanoparticle solution can be an antibacterial alternative for different biomedical approaches.

Bacterial resistance to silver nanoparticles and how to overcome it

2017 ◽  
Vol 13 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Aleš Panáček ◽  
Libor Kvítek ◽  
Monika Smékalová ◽  
Renata Večeřová ◽  
Milan Kolář ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Resistance

scholarly journals Antimicrobial applications of nanoliposome encapsulated silver nanoparticles: a potential strategy to overcome bacterial resistance

2020 ◽  
Vol 16 ◽  
Author(s):  
M.R. Mozafari ◽  
Sarabanou Torkaman ◽  
Fatemeh Mahsa Karamouzian ◽  
Babak Rasti ◽  
Bikash Baral
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Infections ◽  
Bacterial Resistance ◽  
Water Soluble ◽  
Severe Illness ◽  
Efficient System ◽  
Antimicrobial Substances ◽  
Potential Strategy

: Bacterial infections result in hundreds of million cases of severe illness annually worldwide. Rapidly increasing drug resistance of pathogens further aggravates this threat to human health and warrants the search for effective broadspectrum antibacterial agents. Silver metal has a long history of application in human medicine and healthcare. In ancient times, silver was employed as a disinfectant for water purification and storage while it is still being used as an antimicrobial ingredient in some nanotechnology-based products. Encapsulation of antimicrobial substances such as silver nanoparticles in nanoliposomes could provide protection and targeting for the encapsulated or entrapped material. Nanoliposomes are biocompatible and biodegradable drug delivery systems with the ability to encapsulate both lipidsoluble and water-soluble compounds, as well as metal ions. Furthermore, nanoliposomes have been shown to be able to deliver encapsulated agents to target bacteria in vitro as well as in vivo. In this review, we present the use of nanoliposome-encapsulated silver nanoparticles as an efficient system for antibacterial applications.

scholarly journals Enhanced Antibacterial Activity of Poly (dimethylsiloxane) Membranes by Incorporating SiO2 Microspheres Generated Silver Nanoparticles

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 705 ◽  
Author(s):  
Qihui Shen ◽  
Yixuan Shan ◽  
Yang Lü ◽  
Peng Xue ◽  
Yan Liu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Bacterial Resistance ◽  
Click Reaction ◽  
Low Cost ◽  
Thiol Group ◽  
Water Contact ◽  
Stabilizing Agents ◽  
Wide Range ◽  
Sio2 Microspheres

The nonspecific adsorption of proteins and bacteria on the surface of polydimethylsiloxane (PDMS) had been a serious concern in a wide range of applications, such as medical devices. In order to improve the anti-adhesive and antibacterial capability, bare silver nanoparticles (AgNPs, ~15 nm) were generated in-situ on their surface without extra reducing and stabilizing agents. The main reason for this was that the SiO2 microspheres that are covalent bonded to the bulked PDMS could not only generate AgNPs spontaneously but also insure that no AgNPs were released to the environment. Meanwhile, the thiol-group-functionalized SiO2 microspheres self-assembled on the surface of PDMS by thiol-vinyl click reaction without any impact on their biomedical applications. After the modification of SiO2 microspheres with AgNPs, the surface of PDMS showed a smaller water contact angle than before, and the adhesion and growth of E. coli and Bacillus subtilis were effectively inhibited. When the monolayer of SiO2 microspheres with AgNPs was assembled completely on the surface of PDMS, they present improved bacterial resistance performance (living bacteria, 0%). This approach offers an antibacterial and anti-adhesive surface bearing small and well-defined quantities of in-situ generated AgNPs, and it is a novel, green, simple, and low-cost technique to generate AgNPs on soft biomedical substrates.

scholarly journals Bactericidal Properties of Bacillus Subtilis Nanoparticles Against Selected Human Pathogens

2020 ◽  
Vol 1 (5) ◽  
Author(s):  
A. O. Daniels ◽  
J. K. Fadairo ◽  
A. O. Fashoyin
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Silver Nanoparticles ◽  
Bacillus Subtilis ◽  
Bacterial Resistance ◽  
Antibacterial Properties ◽  
Resistance Index ◽  
High Rate ◽  
Resistance To Antibiotics ◽  
Antibiotic Susceptibility Test

The use of biologically synthesized nanoparticles has been an area of research interest in recent times. Due to the high rate of bacterial resistance to antibiotics, there is a need to search for a more potent alternative to ineffective antibiotics. This study aims to evaluate the antibacterial effects of silver nanoparticles synthesized by Bacillus subtilis against Pseudomonas aeruginosa and Staphylococcus aureus. Silver nanoparticles were obtained by dissolving 0.842 gram of AgNO3 silver nitrate into 100ml of B. subtilis in Mueller Hinton broth. The antibacterial susceptibility of the nanoparticles formed was carried out using standard methods. Comparative antibacterial test was also carried out using standard antibiotics The multiple antibiotic resistance index were also determined. The zones of inhibition were 29 and 12 mm against Staphylococcus aureus and Pseudomonas aeruginosa respectively after 8 hrs of nanoparticle synthesis. The antibiotic susceptibility test using standard antibiotics revealed that S. aureus was sensitive to only Erythromycin and ofloxacin with a zone of inhibition of 15mm and 9mm respectively while P. aeruginosa was sensitive only to ofloxacin. The Multiple resistance index (MARi) shows P aeruginosa to have MARi of 0.9 while S, aureus has MARi of 0.82. The result indicated that B. subtilis nanoparticles presented better antibacterial properties than standard antibiotic and can be explored as a candidate for drug production to fight bacterial resistance to antibiotics.

scholarly journals Silver nanoparticles: an ecological method of synthesis, properties and use against antibiotic resistant microflora

2021 ◽  
Vol 18 (3) ◽  
pp. 351-361
Author(s):  
R. I. Dovnar ◽  
A. Yu. Vasil’kov ◽  
T. M. Sakalova ◽  
A. V. Naumkin ◽  
A. V. Budnikov ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
Bacterial Resistance ◽  
Antibacterial Effect ◽  
Synthesis Method ◽  
Antibacterial Properties ◽  
Average Diameter ◽  
Dilution Method ◽  
Antimicrobial Drugs ◽  
Gram Negative

A quantitative assessment of the antibacterial effect of silver nanoparticles on polyantibiotic-resistant grampositive and gram-negative microorganisms was carried out. Silver nanoparticles were synthesized by the environmentally friendly metal-steam synthesis method. The size and electronic state of nanoparticles were investigated by transmission electron and X-ray photoelectron spectroscopy. The antibacterial properties of nanomaterials were assessed on two clinical pathogenic strains of gram-positive and four strains of gram-negative microorganisms. The typing and assessment of bacterial resistance to antibiotics were carried out on a microbiological analyzer. The antibacterial effect of nanoparticles was quantitatively assessed using the dilution method and the determination of the minimum inhibitory and minimum bactericidal concentrations.It was found that the studied silver nanoparticles have sizes in the range from 5 to 24 nm with an average diameter of 10.8 nm. It was shown that all clinical strains of microorganisms used in the study are characterized by multiple antibacterial resistance; the percentage of their antibiotic resistance ranges from 12.5 to 93.3 %. It was found that for the studied microorganism, the values of the minimum inhibitory concentration (MIC) are in the range from 7.81 to 31.25 μg/ml, and the minimum bactericidal concentration (MBC) is in the range from 31.25 to 62.50 μg/ml. The obtained MIC and MBC data can be used to create promising antimicrobial drugs and medical next generation devices.

Spondias tuberosa Extract for Silver Nanoparticles Assisted Synthesis Against Multiresistant Bacteria

2019 ◽  
Vol 11 (11) ◽  
pp. 1041-1048
Author(s):  
José Reinaldo Oliveira Silva ◽  
Anne Caroline Santos Ramos ◽  
Zenon Machado Lima ◽  
Bruno Dos Santos Lima ◽  
Adriano Antunes de Souza Araújo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Bacterial Resistance ◽  
Nanoparticle Synthesis ◽  
Antimicrobial Effect ◽  
Resistant Bacteria ◽  
Phytochemical Analysis ◽  
Minimal Risk ◽  
Multiresistant Bacteria ◽  
Spondias Tuberosa

Multiresistant bacteria represent a global health problem, encouraging the development of effective antimicrobial treatments. In this context, metallic nanoparticles like silver nanoparticles (AgNPs) can serve as an alternative to the pathogenic bacterial growth control since AgNPs have minimal risk of promoting bacterial resistance. The chemical methods to synthesize AgNPs involves toxic agents however, an alternative way to obtain them would be employing plant extract due to its redox ability. In the current study, Spondias tuberosa hydroethanolic leaf extract (StHE) was used to assist silver nanoparticle synthesis (AgNP) and to evaluate the antimicrobial effect of both StHE and AgNP against antibiotic-resistant bacterias Pseudomonas aeruginosa and Staphylococcus aureus. The StHE phytochemical analysis displayed significant polyphenol and flavonoids contents, identified by HPLC. Synthesized AgNPs were spherical with an average size of 3.44–19.46 nm, characterized by UV-Vis and transmission electron microscopy (TEM). The AgNPs antimicrobial activity displayed higher capacity than the crude StHE, mainly against Gram-negative bacteria. Therefore, StHE is an effective source of reducing agents for the AgNPs synthesis, which exhibit high antibacterial potential against multidrug-resistant bacteria.

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