scholarly journals Label‐Free Near‐Infrared Plasmonic Sensing Technique for DNA Detection at Ultralow Concentrations

2020 ◽  
Vol 7 (23) ◽  
pp. 2000763
Author(s):  
Shimeng Chen ◽  
Chuan Liu ◽  
Yun Liu ◽  
Qiang Liu ◽  
Mengdi Lu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Dna Detection ◽  
Label Free ◽  
Plasmonic Sensing ◽  
Ultralow Concentrations

scholarly journals Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Soo-Yeon Cho ◽  
Xun Gong ◽  
Volodymyr B. Koman ◽  
Matthias Kuehne ◽  
Sun Jin Moon ◽  
...  
Keyword(s):  
Near Infrared ◽  
Single Cells ◽  
Microfluidic Channel ◽  
Human Monocyte ◽  
Cellular Heterogeneity ◽  
Label Free ◽  
Nanotube Array ◽  
Chemical Cytometry ◽  
Rich Information ◽  
Non Destructive

AbstractNanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of ~600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.

scholarly journals Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 922
Author(s):  
William Querido ◽  
Shital Kandel ◽  
Nancy Pleshko
Keyword(s):  
Raman Spectroscopy ◽  
Vibrational Spectroscopy ◽  
Near Infrared ◽  
Ex Vivo ◽  
Biological Tissues ◽  
Label Free ◽  
Connective Tissues ◽  
Bone Properties ◽  
Composition And Structure

Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.

A low-cost, label-free DNA detection method in lab-on-chip format based on electrohydrodynamic instabilities, with application to long-range PCR

Lab on a Chip ◽  
2012 ◽  
Vol 12 (22) ◽  
pp. 4738 ◽  
Author(s):  
Mohamed Lemine Youba Diakité ◽  
Jerôme Champ ◽  
Stephanie Descroix ◽  
Laurent Malaquin ◽  
François Amblard ◽  
...  
Keyword(s):  
Long Range ◽  
Detection Method ◽  
Dna Detection ◽  
Low Cost ◽  
Label Free ◽  
Lab On Chip ◽  
Free Dna ◽  
Chip Format ◽  
Long Range Pcr ◽  
On Chip

scholarly journals A one-step and label-free, electrochemical DNA detection using metal ion-mediated molecular beacon probe

2019 ◽  
Vol 100 ◽  
pp. 64-69 ◽  
Author(s):  
Songyi Baek ◽  
Jun Ki Ahn ◽  
Byoung Yeon Won ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Metal Ion ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Label Free ◽  
One Step ◽  
Electrochemical Dna Detection

Fabrication of Precise Asymmetric Nanoshells Array with Nanogaps for a Label-Free Immunoassay Based on NIR-Light Responsive LSPR

2012 ◽  
Vol 523-524 ◽  
pp. 680-685
Author(s):  
Shuhei Uchida ◽  
Kazuya Yamamura ◽  
Nobuyuki Zettsu
Keyword(s):  
Near Infrared ◽  
Atmospheric Pressure Plasma ◽  
High Sensitivity ◽  
Medical Diagnostics ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Fabrication Technique ◽  
Alternative Structures ◽  
Wide Range ◽  
Surrounding Refractive Index

Localized surface plasmon resonance (LSPR) based sensors are a well established technology utilized for label-free biochemical sensing in immunoassay, medical diagnostics and environmental monitoring. The understanding of asymmetric metal nanoparticles, new object for complex, coupled plasmon systems providing localized significantly enhanced E-field, is central to a wide range of novel applications and processes in science of higher sensitive sensing systems. However, few methods are available for actual characterization of such nanostructures at the single particle level. Here we propose a precise and large sized scale fabrication technique for asymmetric nanoshells array with nanogaps of several tens of nanometers for LSPR sensor through atmospheric pressure plasma etching processes. A nanoshell was simply constructed by laminating thin Au films on periodic isolated polymer nanoparticles template. This nanoshells array was expected to exhibit specific near-infrared plasmonic properties. When measuring the sensitivity, nanoshells array exhibited a high sensitivity to changes of surrounding refractive index and showed a higher sensor figure of merit than the alternative structures. This indicated that the enhanced plasmon E-field in the asymmetric nanostructures improved sensor performance. Our fabrication technique and the optical properties of the arrays will provide useful information for developing new plasmonic applications.

Label-free DNA detection based on a novel nanostructured conducting poly(indole-6-carboxylic acid) films

2009 ◽  
Vol 139 (2) ◽  
pp. 592-597 ◽  
Author(s):  
Guangming Nie ◽  
Yan Zhang ◽  
Qingfu Guo ◽  
Shusheng Zhang
Keyword(s):  
Carboxylic Acid ◽  
Dna Detection ◽  
Label Free ◽  
Free Dna
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