scholarly journals Quantitative Mapping of Liver Hypoxia in Living Mice Using Time‐Resolved Wide‐Field Phosphorescence Lifetime Imaging

2020 ◽  
Vol 7 (11) ◽  
pp. 1902929 ◽  
Author(s):  
Yawei Liu ◽  
Yuyang Gu ◽  
Wei Yuan ◽  
Xiaobo Zhou ◽  
Xiaochen Qiu ◽  
...  
Keyword(s):  
Wide Field ◽  
Time Resolved ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Quantitative Mapping ◽  
Phosphorescence Lifetime Imaging

scholarly journals 3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation

2012 ◽  
Vol 20 (24) ◽  
pp. 26219 ◽  
Author(s):  
Heejin Choi ◽  
Dimitrios S. Tzeranis ◽  
Jae Won Cha ◽  
Philippe Clémenceau ◽  
Sander J. G. de Jong ◽  
...  
Keyword(s):  
Wide Field ◽  
Phosphorescence Lifetime ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Photon Excitation ◽  
Temporal Focusing ◽  
Phosphorescence Lifetime Imaging ◽  
Fluorescence And Phosphorescence ◽  
Two Photon Excitation

scholarly journals Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2898
Author(s):  
Ilya S. Kritchenkov ◽  
Anastasia I. Solomatina ◽  
Daria O. Kozina ◽  
Vitaly V. Porsev ◽  
Victor V. Sokolov ◽  
...  
Keyword(s):  
Near Infrared ◽  
Biological Systems ◽  
Aqueous Media ◽  
Oxygen Sensors ◽  
Quantum Yields ◽  
Complete Agreement ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Nir Emission ◽  
Phosphorescence Lifetime Imaging

Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry. We have designed and synthetized four novel iridium [Ir(N^C)2(N^N)]+ complexes (N^C–benzothienyl-phenanthridine based cyclometalated ligand; N^N–pyridin-phenanthroimidazol diimine chelate), decorated with oligo(ethylene glycol) groups to impart these emitters’ solubility in aqueous media, biocompatibility, and to shield them from interaction with bio-environment. These substances were fully characterized using NMR spectroscopy and ESI mass-spectrometry. The complexes exhibited excitation close to the biological “window of transparency”, NIR emission at 730 nm, and quantum yields up to 12% in water. The compounds with higher degree of the chromophore shielding possess low toxicity, bleaching stability, absence of sensitivity to variations of pH, serum, and complex concentrations. The properties of these probes as oxygen sensors for biological systems have been studied by using phosphorescence lifetime imaging experiments in different cell cultures. The results showed essential lifetime response onto variations in oxygen concentration (2.0–2.3 μs under normoxia and 2.8–3.0 μs under hypoxia conditions) in complete agreement with the calibration curves obtained “in cuvette”. The data obtained indicate that these emitters can be used as semi-quantitative oxygen sensors in biological systems.

scholarly journals Water-soluble cyclometalated platinum(ii) and iridium(iii) complexes: synthesis, tuning of the photophysical properties, and in vitro and in vivo phosphorescence lifetime imaging

RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17224-17236 ◽  
Author(s):  
Anastasia I. Solomatina ◽  
Shih-Hao Su ◽  
Maria M. Lukina ◽  
Varvara V. Dudenkova ◽  
Vladislav I. Shcheslavskiy ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Water Soluble ◽  
Iridium Complexes ◽  
Hypoxia Imaging ◽  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

Novel water-soluble iridium complexes with sulfonated diphosphine allow in vitro and in vivo lifetime hypoxia imaging.

Phosphorescence Lifetime Imaging of Labile Zn2+ in Mitochondria via a Phosphorescent Iridium(III) Complex

2018 ◽  
Vol 57 (17) ◽  
pp. 10625-10632 ◽  
Author(s):  
Changli Zhang ◽  
Minsheng Liu ◽  
Shaoxian Liu ◽  
Hui Yang ◽  
Qiang Zhao ◽  
...  
Keyword(s):  
Phosphorescence Lifetime ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

scholarly journals A pilot study of the dynamics of tissue oxygenation in vivo using time-resolved phosphorescence imaging

2021 ◽  
pp. 2142001
Author(s):  
Natalia Kazachkina ◽  
Julia Lymar ◽  
Vladislav Shcheslavskiy ◽  
Alexander Savitsky
Keyword(s):  
Solid Tumor ◽  
Single Photon ◽  
Photon Counting ◽  
Time Resolved ◽  
Phosphorescence Lifetime ◽  
Phosphorescence Quenching ◽  
Single Photon Counting ◽  
Normal Tissues ◽  
Phosphorescence Lifetime Imaging

Oxygenation of tissues plays an important role in the development and progression of tumor to treatment effects. Method of metalloporphyrines phosphorescence quenching by oxygen is one of the ways to measure dynamics of the oxygen concentration in the tissues by phosphorescence lifetime imaging of meso-tetra(sulfopheny1)tetrabenzoporphyrin Pd (II) (TBP) using the time-correlated single photon counting (TCSPC) method. It has been shown that phosphorescence lifetime of the sensor in S37 tumor in vivo varied in the range of 130 to 290 [Formula: see text]s after both topical and intravenous administration of TBP. It indicates that oxygen level in tumors was lower compared to normal tissues where TBP phosphorescence has not been detected. Phosphorescence lifetimes of TBP increased in the solid tumor and in the muscle after photodynamic therapy of solid tumor that demonstrates oxygen consumption during treatment and possibly stopping the blood flow and hence the oxygen supply to the tissues.

scholarly journals Monitoring mitochondrial viscosity with anticancer phosphorescent Ir(iii) complexes via two-photon lifetime imaging

2019 ◽  
Vol 10 (5) ◽  
pp. 1285-1293 ◽  
Author(s):  
Liang Hao ◽  
Zhi-Wei Li ◽  
Dong-Yang Zhang ◽  
Liang He ◽  
Wenting Liu ◽  
...  
Keyword(s):  
Mitochondrial Dysfunction ◽  
Phosphorescence Lifetime ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Phosphorescence Lifetime Imaging

A theranostic Ir(iii) complex is developed to induce mitochondrial dysfunction and monitor mitochondrial viscosity quantitatively via two-photon phosphorescence lifetime imaging.

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