scholarly journals Targeting Antitumoral Proteins to Breast Cancer by Local Administration of Functional Inclusion Bodies

2019 ◽  
Vol 6 (18) ◽  
pp. 1900849 ◽  
Author(s):  
Mireia Pesarrodona ◽  
Toni Jauset ◽  
Zamira V. Díaz‐Riascos ◽  
Alejandro Sánchez‐Chardi ◽  
Marie‐Eve Beaulieu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Inclusion Bodies ◽  
Local Administration ◽  
Functional Inclusion

scholarly journals Positive in vitro wound healing effects of functional inclusion bodies of a lipoxygenase from the Mexican axolotl

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Anne Stamm ◽  
Sarah Strauß ◽  
Peter Vogt ◽  
Thomas Scheper ◽  
Iliyana Pepelanova
Keyword(s):  
Wound Healing ◽  
Inclusion Bodies ◽  
Mexican Axolotl ◽  
Functional Inclusion

Nanopills: Functional Inclusion Bodies Produced in Bacteria as Naturally Occurring Nanopills for Advanced Cell Therapies (Adv. Mater. 13/2012)

2012 ◽  
Vol 24 (13) ◽  
pp. 1741-1741
Author(s):  
Esther Vázquez ◽  
José L. Corchero ◽  
Joan F. Burgueño ◽  
Joaquin Seras-Franzoso ◽  
Ana Kosoy ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
Cell Therapies ◽  
Naturally Occurring ◽  
Functional Inclusion

scholarly journals Coiled-coil inspired functional inclusion bodies

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Marcos Gil-Garcia ◽  
Susanna Navarro ◽  
Salvador Ventura
Keyword(s):  
Inclusion Bodies ◽  
Coiled Coil ◽  
Functional Inclusion

scholarly journals The Arabian camel,Camelus dromedariusinterferon epsilon: functional expression,in vitrorefolding, purification and cytotoxicity on breast cancer cell lines MDA-MB-231 and MCF-7

2019 ◽  
Author(s):  
Manal Abdel-Fattah ◽  
Hesham Saeed ◽  
Lamiaa El-Shennawy ◽  
Manal Shalaby ◽  
Amira M. Embaby ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Inclusion Bodies ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Dependent Manner ◽  
Arabian Camel ◽  
Mcf 7

AbstractThe current study highlights for the first time cloning, overexpression, purification, and assessing the cytotxcity of the novel interferon epsilon (IFNε), from the Arabian camelCamelus dromedarius, against two human breast cancer cell lines MDA-MB-231 and MCF-7. Full-length cDNA encoding interferon epsilon (IFNε) was isolated and cloned from the liver of the Arabian camel,C. dromedariususing reverse transcription-polymerase chain reaction. The sequence analysis of the camel IFNε cDNA showed a 582-bp open reading frame encoding a protein of 193 amino acids with an estimated molecular weight of 22.953 kDa. A BLAST search analysis revealed that theC. dromedariusIFNε shared high sequence identity with the IFN genes of other species, such asCamelus ferus,Vicugna pacos, andHomo sapiens. Expression of the camel IFNε cDNA inEscherichia coligave a fusion protein band of 22.73 kDa after induction with either isopropyl β-D-1-thiogalactopyranoside or lactose for 5 h. Recombinant IFNε protein was overexpressed in the form of inclusion bodies that were easily solubilized and refolded using SDS and KCl. The solubilized inclusion bodies were purified to apparent homogeneity using nickel affinity chromatography. We examined the effect of IFNε on two breast cancer cell lines MDA-MB-231 and MCF-7. In both cell lines, IFNε inhibited cell survival in a dose dependent manner as observed by MTT assay, morphological changes and apoptosis assay. Caspase-3 expression level was found to be increased in MDA-MB-231 treated cells as compared to untreated cells.

scholarly journals Production of functional inclusion bodies in endotoxin-free Escherichia coli

2014 ◽  
Vol 98 (22) ◽  
pp. 9229-9238 ◽  
Author(s):  
Fabián Rueda ◽  
Olivia Cano-Garrido ◽  
Uwe Mamat ◽  
Kathleen Wilke ◽  
Joaquin Seras-Franzoso ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Functional Inclusion

Enzyme assembly guided by SPFH‐induced functional inclusion bodies for enhanced cascade biocatalysis

2020 ◽  
Vol 117 (5) ◽  
pp. 1446-1457 ◽  
Author(s):  
Xueqin Lv ◽  
Ke Jin ◽  
Yaokang Wu ◽  
Cheng Zhang ◽  
Shixiu Cui ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
Cascade Biocatalysis ◽  
Functional Inclusion

scholarly journals Promotion of Local Talents for Breast Cancer Screening by Support of Local Administration

2013 ◽  
Vol 22 (1) ◽  
pp. 45-50
Author(s):  
Takayuki Kadoya ◽  
Etsushi Akimoto ◽  
Junko Emi ◽  
Hideo Shigematsu ◽  
Norio Masumoto ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Screening ◽  
Breast Cancer Screening ◽  
Local Administration

scholarly journals Functional inclusion bodies produced in the yeast Pichia pastoris

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Fabián Rueda ◽  
Brigitte Gasser ◽  
Alejandro Sánchez-Chardi ◽  
Mònica Roldán ◽  
Sandra Villegas ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Inclusion Bodies ◽  
Yeast Pichia Pastoris ◽  
Functional Inclusion
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