scholarly journals Multimeric Amphipathic α-Helical Sequences for Rapid and Efficient Intracellular Protein Transport at Nanomolar Concentrations

2018 ◽  
Vol 5 (8) ◽  
pp. 1800240 ◽  
Author(s):  
Jae Hoon Oh ◽  
Seung-Eun Chong ◽  
Sohee Nam ◽  
Soonsil Hyun ◽  
Sejong Choi ◽  
...  
Keyword(s):  
Protein Transport ◽  
Intracellular Protein ◽  
Intracellular Protein Transport

scholarly journals Exosomal Bta-miR-185 and Bta-miR-378 Are Potential Markers for Quality Control of Milk Infected by Staphylococcus aureus

2019 ◽  
Author(s):  
Shaoyang Ma ◽  
Chao Tong ◽  
Eveline M. Ibeagha-Awemu ◽  
Xin Zhao
Keyword(s):  
Staphylococcus Aureus ◽  
Quality Control ◽  
Endoplasmic Reticulum ◽  
Target Gene ◽  
Protein Transport ◽  
Target Genes ◽  
Intracellular Protein ◽  
Intracellular Protein Transport ◽  
Mastitic Milk ◽  
Potential Biomarkers

Abstract MicroRNAs (miRNA) in milk-derived exosomes may reflect pathophysiological changes caused by mastitis. This study profiled miRNAs in milk exosomes and potential markers for quality control of milk infected by Staphylococcus aureus (S. aureus). Total RNA from milk exosomes was collected from healthy cows (n=3) and S. aureus infected cows (n=6). Two hundred ninety miRNAs (221 known and 69 novel ones) were identified. Among them, 22 known and 15 novel miRNAs were differentially expressed (DE). Target genes of DE miRNAs were significantly enriched in intracellular protein transport, endoplasmic reticulum and identical protein binding. Two miRNAs (bta-miR-378 and bta-miR-185) with high read counts and log2 fold changes (>3.5) were considered as potential milk exosome biomarker miRNAs for S. aureus mastitis and milk quality. One target gene (VAT1L) of bta-miR-378 and five target genes (DYRK1B, MLLT3, HP1BP3, NPR2 and PGM1) of bta-miR-185 were validated. DE miRNAs in exosomes from normal and S. aureus infected milk were identified. Two DE miRNAs could be used as potential biomarkers for the infection. The linkage between the validated target genes and diseases suggest that we should pay particular attention to exosome miRNAs from mastitic milk in terms of milk safety.

scholarly journals Meiotic maturation in Xenopus oocytes: a link between the cessation of protein secretion and the polarized disappearance of Golgi apparati.

1985 ◽  
Vol 101 (1) ◽  
pp. 313-318 ◽  
Author(s):  
A Colman ◽  
E A Jones ◽  
J Heasman
Keyword(s):  
Xenopus Oocytes ◽  
Protein Transport ◽  
Meiotic Metaphase ◽  
Meiotic Maturation ◽  
Detectable Effect ◽  
Xenopus Laevis Oocytes ◽  
Intracellular Protein ◽  
Intracellular Protein Transport ◽  
The Relationship ◽  
Polyadenylated Mrna

We have studied the relationship between the timing of the late meiotic events that occur during progesterone-induced oocyte maturation, and intracellular protein transport. We have monitored the secretion of chick oviduct proteins from Xenopus laevis oocytes microinjected with polyadenylated mRNA and found that chick ovalbumin and lysozyme are not secreted during the second meiotic metaphase, in contrast to the earlier prophase stage. Maturation had no detectable effect on the glycosylation of ovalbumin, whereas it affected the glycosylation of chick ovomucoid. As maturation proceeded, the Golgi apparati disappeared in a polarized fashion, beginning in the vegetal half. This disappearance coincided temporally and spatially with that of the nuclear envelope. We speculate that Golgi apparatus disappearance and the block in secretion are causally related.

pH-induced intracellular protein transport

2006 ◽  
Vol 3 (2) ◽  
pp. 101-106 ◽  
Author(s):  
E M Baskin ◽  
S Bukshpan ◽  
G V Zilberstein
Keyword(s):  
Protein Transport ◽  
Intracellular Protein ◽  
Intracellular Protein Transport
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