Simulation Assisted Nanoscale Imaging of Single Live Cells with Scanning Electrochemical Microscopy

2018 ◽  
Vol 2 (2) ◽  
pp. 1800124 ◽  
Author(s):  
Fraser P. Filice ◽  
Michelle S. M. Li ◽  
Zhifeng Ding
Keyword(s):  
Scanning Electrochemical Microscopy ◽  
Live Cells ◽  
Nanoscale Imaging ◽  
Electrochemical Microscopy

Analysing single live cells by scanning electrochemical microscopy

The Analyst ◽  
2019 ◽  
Vol 144 (3) ◽  
pp. 738-752 ◽  
Author(s):  
Fraser P. Filice ◽  
Zhifeng Ding
Keyword(s):  
Scanning Electrochemical Microscopy ◽  
Live Cell ◽  
Live Cells ◽  
Cellular Physiology ◽  
Electrochemical Microscopy

Scanning electrochemical microscopy (SECM) offers single live cell activities along its topography toward cellular physiology and pathology.

Comparison study of live cells by atomic force microscopy, confocal microscopy, and scanning electrochemical microscopy

2007 ◽  
Vol 85 (3) ◽  
pp. 175-183 ◽  
Author(s):  
Xiaocui Zhao ◽  
Nils O Petersen ◽  
Zhifeng Ding
Keyword(s):  
Atomic Force Microscopy ◽  
Confocal Microscopy ◽  
Scanning Electrochemical Microscopy ◽  
Time Lapse ◽  
Live Cells ◽  
Tapping Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tapping Mode Afm ◽  
Electrochemical Microscopy

In this report, three kinds of scanning probe microscopy techniques, atomic force microscopy (AFM), confocal microscopy (CM), and scanning electrochemical microscopy (SECM), were used to study live cells in the physiological environment. Two model cell lines, CV-1 and COS-7, were studied. Time-lapse images were obtained with both contact and tapping mode AFM techniques. Cells were more easily scratched or moved by contact mode AFM than by tapping mode AFM. Detailed surface structures such as filamentous structures on the cell membrane can be obtained and easily discerned with tapping mode AFM. The toxicity of ferrocenemethanol (Fc) on live cells was studied by CM in reflection mode by recording the time-lapse images of controlled live cells and live cells with different Fc concentrations. No significant change in the morphology of cells was caused by Fc. Cells were imaged by SECM with Fc as the mediator at a biased potential of 0.35 V (vs. Ag/AgCl with a saturated KCl solution). Cells did not change visibly within 1 h, which indicated that SECM was a noninvasive technique and thus has a unique advantage for the study of soft cells, since the electrode scanned above the cells instead of in contact with them. Reactive oxygen species (ROS) generated by the cells were detected and images based on these chemical species were obtained. It is demonstrated that SECM can provide not only the topographical images but also the images related to the chemical or biochemical species released by the live cells.Key words: live cells, atomic force microscopy, confocal microscopy, scanning electrochemical microscopy.

Sensing cisplatin-induced permeation of single live human bladder cancer cells by scanning electrochemical microscopy

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6054-6060 ◽  
Author(s):  
Meng-Ni Zhang ◽  
Zhifeng Ding ◽  
Yi-Tao Long
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Scanning Electrochemical Microscopy ◽  
Live Cells ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Simultaneous Quantification ◽  
Bladder Cancer Cells ◽  
Human Bladder Cancer Cells ◽  
Electrochemical Microscopy

Simultaneous quantification of cellular topography and membrane permeability of single live cells was realized by means of scanning electrochemical microscopy.

scholarly journals Glucose and Lactate Biosensors for Scanning Electrochemical Microscopy Imaging of Single Live Cells

2008 ◽  
Vol 80 (8) ◽  
pp. 2717-2727 ◽  
Author(s):  
Madalina Ciobanu ◽  
Dale E. Taylor ◽  
Jeremy P. Wilburn ◽  
David E. Cliffel
Keyword(s):  
Scanning Electrochemical Microscopy ◽  
Live Cells ◽  
Microscopy Imaging ◽  
Electrochemical Microscopy

scholarly journals Use of Amperometric and Potentiometric Probes in Scanning Electrochemical Microscopy for the Spatially-Resolved Monitoring of Severe Localized Corrosion Sites on Aluminum Alloy 2098-T351

Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1132 ◽  
Author(s):  
Rejane M. P. da Silva ◽  
Javier Izquierdo ◽  
Mariana X. Milagre ◽  
Abenchara M. Betancor-Abreu ◽  
Isolda Costa ◽  
...  
Keyword(s):  
Scanning Electrochemical Microscopy ◽  
Oxygen Depletion ◽  
Localized Corrosion ◽  
Alloy Surface ◽  
Al Alloy ◽  
Spatially Resolved ◽  
Lower Oxygen ◽  
Local Ph ◽  
Electrochemical Microscopy

Amperometric and potentiometric probes were employed for the detection and characterization of reactive sites on the 2098-T351 Al-alloy (AA2098-T351) using scanning electrochemical microscopy (SECM). Firstly, the probe of concept was performed on a model Mg-Al galvanic pair system using SECM in the amperometric and potentiometric operation modes, in order to address the responsiveness of the probes for the characterization of this galvanic pair system. Next, these sensing probes were employed to characterize the 2098-T351 alloy surface immersed in a saline aqueous solution at ambient temperature. The distribution of reactive sites and the local pH changes associated with severe localized corrosion (SLC) on the alloy surface were imaged and subsequently studied. Higher hydrogen evolution, lower oxygen depletion and acidification occurred at the SLC sites developed on the 2098-T351 Al-alloy.

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