scholarly journals Cowpea Mosaic Virus Promotes Anti‐Tumor Activity and Immune Memory in a Mouse Ovarian Tumor Model

2019 ◽  
Vol 2 (5) ◽  
pp. 1900003 ◽  
Author(s):  
Chao Wang ◽  
Steven N. Fiering ◽  
Nicole F. Steinmetz
Keyword(s):  
Mosaic Virus ◽  
Ovarian Tumor ◽  
Tumor Model ◽  
Immune Memory ◽  
Cowpea Mosaic Virus ◽  
Anti Tumor Activity

scholarly journals Active Delivery of VLPs Promotes Anti‐Tumor Activity in a Mouse Ovarian Tumor Model

Small ◽  
2020 ◽  
Vol 16 (20) ◽  
pp. 1907150 ◽  
Author(s):  
Chao Wang ◽  
Berta Esteban Fernández de Ávila ◽  
Rodolfo Mundaca‐Uribe ◽  
Miguel Angel Lopez‐Ramirez ◽  
Doris E. Ramírez‐Herrera ◽  
...  
Keyword(s):  
Ovarian Tumor ◽  
Tumor Model ◽  
Anti Tumor Activity

scholarly journals Capsid Proteins of Cowpea Mosaic Virus Transiently Expressed in Protoplasts Form Virus-like Particles

Virology ◽  
1996 ◽  
Vol 224 (1) ◽  
pp. 352-355 ◽  
Author(s):  
Joan Wellink ◽  
Jan Verver ◽  
Jan Van Lent ◽  
A.Van Kammen
Keyword(s):  
Mosaic Virus ◽  
Capsid Proteins ◽  
Cowpea Mosaic Virus ◽  
Virus Like Particles

scholarly journals Cowpea mosaic virus nanoparticles target surface vimentin on cancer cells

Nanomedicine ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. 351-364 ◽  
Author(s):  
Nicole F Steinmetz ◽  
Choi-Fong Cho ◽  
Amber Ablack ◽  
John D Lewis ◽  
Marianne Manchester
Keyword(s):  
Cancer Cells ◽  
Mosaic Virus ◽  
Target Surface ◽  
Cowpea Mosaic Virus

Inactivated Cowpea Mosaic Virus in Combination with OX40 Agonist Primes Potent Antitumor Immunity in a Bilateral Melanoma Mouse Model

2022 ◽  
Author(s):  
Edward C. Koellhoffer ◽  
Chenkai Mao ◽  
Veronique Beiss ◽  
Lu Wang ◽  
Steven N. Fiering ◽  
...  
Keyword(s):  
Mouse Model ◽  
Mosaic Virus ◽  
Antitumor Immunity ◽  
Cowpea Mosaic Virus

scholarly journals Encapsidation of Viral RNA inPicornavirales: Studies on Cowpea Mosaic Virus Demonstrate Dependence on Viral Replication

2018 ◽  
Vol 93 (2) ◽  
Author(s):  
Inga Kruse ◽  
Hadrien Peyret ◽  
Pooja Saxena ◽  
George P. Lomonossoff
Keyword(s):  
Viral Replication ◽  
Mosaic Virus ◽  
Viral Rna ◽  
Cowpea Mosaic Virus ◽  
Rna Packaging ◽  
Functional Link ◽  
Close Coupling ◽  
Genomic Rnas ◽  
Viral Rnas ◽  
Virus Like Particles

ABSTRACTTo elucidate the linkage between replication and encapsidation inPicornavirales, we have taken advantage of the bipartite nature of a plant-infecting member of this order, cowpea mosaic virus (CPMV), to decouple the two processes. RNA-free virus-like particles (empty virus-like particles [eVLPs]) can be generated by transiently coexpressing the RNA-2-encoded coat protein precursor (VP60) with the RNA-1-encoded 24,000-molecular-weight (24K) protease, in the absence of the replication machinery (K. Saunders, F. Sainsbury, and G. P. Lomonossoff, Virology 393:329–337, 2009, https://doi.org/10.1016/j.virol.2009.08.023). We have made use of the ability to produce assembled capsids of CPMV in the absence of replication to examine the putative linkage between RNA replication and packaging in thePicornavirales. We have created a series of mutant RNA-1 and RNA-2 molecules and have assessed the effects of the mutations on both the replication and packaging of the viral RNAs. We demonstrate that mutations that affect replication have a concomitant impact on encapsidation and that RNA-1-mediated replication is required for encapsidation of both RNA-1 and RNA-2. This close coupling between replication and encapsidation provides a means for the specific packaging of viral RNAs. Moreover, we demonstrate that this feature of CPMV can be used to specifically encapsidate custom RNA by placing a sequence of choice between the RNA-2 sequences required for replication.IMPORTANCEThe mechanism whereby members of the orderPicornaviralesspecifically package their genomic RNAs is poorly understood. Research with monopartite members of the order, such as poliovirus, indicated that packaging is linked to replication, although the presence of “packaging signals” along the length of the viral RNA has also been suggested. Thanks to the bipartite nature of the CPMV genome, which allows the manipulation of RNA-1 without modifying RNA-2, we show here that this specificity is due to a functional link between the two processes of viral replication and encapsidation. This has important implications for our understanding of the fundamental molecular biology ofPicornaviralesand opens the door to novel research and therapeutic applications in the field of custom RNA packaging and delivery technologies.

scholarly journals Anti-tumor activity of KNTC2 siRNA in orthotopic tumor model mice of hepatocellular carcinoma

2017 ◽  
Vol 493 (1) ◽  
pp. 800-806 ◽  
Author(s):  
Yukimasa Makita ◽  
Shumpei Murata ◽  
Yoshiki Katou ◽  
Kuniko Kikuchi ◽  
Hiroshi Uejima ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Model ◽  
Orthotopic Tumor ◽  
Orthotopic Tumor Model ◽  
Anti Tumor Activity
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