Site-Directed Mutagenesis Studies on the Toluene Dioxygenase Enzymatic System: Role of Phenylalanine 366, Threonine 365 and Isoleucine 324 in the Chemo-, Regio-, and Stereoselectivity

María Agustina Vila; Diego Umpiérrez; Nicolás Veiga; Gustavo Seoane; Ignacio Carrera; Sonia Rodríguez Giordano

doi:10.1002/adsc.201700444

Site-Directed Mutagenesis Studies on the Toluene Dioxygenase Enzymatic System: Role of Phenylalanine 366, Threonine 365 and Isoleucine 324 in the Chemo-, Regio-, and Stereoselectivity

Advanced Synthesis & Catalysis ◽

10.1002/adsc.201700444 ◽

2017 ◽

Vol 359 (12) ◽

pp. 2149-2157 ◽

Cited By ~ 3

Author(s):

María Agustina Vila ◽

Diego Umpiérrez ◽

Nicolás Veiga ◽

Gustavo Seoane ◽

Ignacio Carrera ◽

...

Keyword(s):

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Enzymatic System ◽

Toluene Dioxygenase

Download Full-text

Probing the role of lysines and arginines in the catalytic function of cytochrome P450d by site-directed mutagenesis. Interaction with NADPH-cytochrome P450 reductase

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)67801-4 ◽

1991 ◽

Vol 266 (6) ◽

pp. 3372-3375

Author(s):

T Shimizu ◽

T Tateishi ◽

M Hatano ◽

Y Fujii-Kuriyama

Keyword(s):

Cytochrome P450 ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Cytochrome P450 Reductase ◽

Nadph Cytochrome ◽

P450 Reductase ◽

Catalytic Function ◽

Nadph Cytochrome P450 Reductase

Download Full-text

The role of cysteine 206 in allosteric inhibition of Escherichia coli citrate synthase. Studies by chemical modification, site-directed mutagenesis, and 19F NMR.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54766-9 ◽

1991 ◽

Vol 266 (31) ◽

pp. 20709-20713

Author(s):

L.J. Donald ◽

B.R. Crane ◽

D.H. Anderson ◽

H.W. Duckworth

Keyword(s):

Escherichia Coli ◽

Chemical Modification ◽

Citrate Synthase ◽

Site Directed Mutagenesis ◽

19F Nmr ◽

Directed Mutagenesis ◽

Allosteric Inhibition

Download Full-text

Role of Cysteine Residues in the N-Terminal Extracellular Domain of the Human VIP 1 Receptor for Ligand Binding A Site-directed Mutagenesis Studya

Annals of the New York Academy of Sciences ◽

10.1111/j.1749-6632.1996.tb17524.x ◽

2006 ◽

Vol 805 (1) ◽

pp. 585-589 ◽

Cited By ~ 4

Author(s):

PASCALE GAUDIN ◽

ALAIN COUVINEAU ◽

JEAN-JOSÉ MAORET ◽

CHRISTIANE ROUYER-FESSARD ◽

MARC LABURTHE

Keyword(s):

Ligand Binding ◽

Extracellular Domain ◽

Site Directed Mutagenesis ◽

Cysteine Residues ◽

Directed Mutagenesis ◽

A Site

Download Full-text

Site-directed mutagenesis of tyrosine hydroxylase. Role of serine 40 in catalysis.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)35673-4 ◽

1992 ◽

Vol 267 (36) ◽

pp. 25754-25758

Author(s):

J Wu ◽

D Filer ◽

A.J. Friedhoff ◽

M Goldstein

Keyword(s):

Tyrosine Hydroxylase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

Download Full-text

Determination of the functional role of phenylalanine-31 of recombinant human dihydrofolate reductase by site-directed mutagenesis

Zurich, Switzerland, September 3–8, 1989 ◽

10.1515/9783110889000-148 ◽

1990 ◽

pp. 765-768

Keyword(s):

Dihydrofolate Reductase ◽

Functional Role ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Human Dihydrofolate Reductase

Download Full-text

The role of cysteines in polyketide synthases. Site-directed mutagenesis of resveratrol and chalcone synthases, two key enzymes in different plant-specific pathways

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)92914-5 ◽

1991 ◽

Vol 266 (15) ◽

pp. 9971-9976 ◽

Cited By ~ 3

Author(s):

T. Lanz ◽

S. Tropf ◽

F.J. Marner ◽

J. Schröder ◽

G. Schröder

Keyword(s):

Polyketide Synthases ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Key Enzymes

Download Full-text

The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)38467-4 ◽

1990 ◽

Vol 265 (20) ◽

pp. 11788-11795

Author(s):

K D Egeberg ◽

B A Springer ◽

S G Sligar ◽

T E Carver ◽

R J Rohlfs ◽

...

Keyword(s):

Ligand Binding ◽

Sperm Whale ◽

Site Directed Mutagenesis ◽

Synthetic Gene ◽

Directed Mutagenesis ◽

Sperm Whale Myoglobin

Download Full-text

Probing the Role of Tyr 64 ofTreponema denticolaCystalysin by Site-Directed Mutagenesis and Kinetic Studies†

Biochemistry ◽

10.1021/bi051433n ◽

2005 ◽

Vol 44 (42) ◽

pp. 13970-13980 ◽

Cited By ~ 17

Author(s):

Barbara Cellini ◽

Mariarita Bertoldi ◽

Riccardo Montioli ◽

Carla Borri Voltattorni

Keyword(s):

Kinetic Studies ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

Download Full-text

The role of cysteine 41 in the enzymatic activities of the pertussis toxin S1 subunit as investigated by site-directed mutagenesis.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)39598-5 ◽

1990 ◽

Vol 265 (8) ◽

pp. 4552-4559 ◽

Cited By ~ 1

Author(s):

C Locht ◽

Y Lobet ◽

C Feron ◽

W Cieplak ◽

J M Keith

Keyword(s):

Pertussis Toxin ◽

Enzymatic Activities ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

Download Full-text

Role of invariant tyrosines in a crustacean mu-class glutathione S-transferase from shrimp Litopenaeus vannamei: Site-directed mutagenesis of Y7 and Y116

Biochimie ◽

10.1016/j.biochi.2008.02.005 ◽

2008 ◽

Vol 90 (6) ◽

pp. 968-971 ◽

Cited By ~ 5

Author(s):

Carmen A. Contreras-Vergara ◽

Elisa M. Valenzuela-Soto ◽

Aldo A. Arvizu-Flores ◽

Rogerio R. Sotelo-Mundo ◽

Gloria Yepiz-Plascencia

Keyword(s):

Litopenaeus Vannamei ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Glutathione S Transferase

Download Full-text