Exploitation of a Laccase/Meldola’s Blue System for NAD+Regeneration in Preparative Scale Hydroxysteroid Dehydrogenase-Catalyzed Oxidations

2012 ◽  
Vol 354 (14-15) ◽  
pp. 2821-2828 ◽  
Author(s):  
Erica Elisa Ferrandi ◽  
Daniela Monti ◽  
Ilabahen Patel ◽  
Roman Kittl ◽  
Dietmar Haltrich ◽  
...  
Keyword(s):  
Hydroxysteroid Dehydrogenase ◽  
Preparative Scale ◽  
Meldola's Blue ◽  
Meldola’S Blue

scholarly journals Electrocatalytic Activity of Electropolymerized Meldola's Blue toward Oxidation of Dopamine

2006 ◽  
Vol 74 (1) ◽  
pp. 32-41 ◽  
Author(s):  
Takahiro YAMAGUCHI ◽  
Teruhisa KOMURA ◽  
Sayomi HAYASHI ◽  
Manabu ASANO ◽  
Guang Yao NIU ◽  
...  
Keyword(s):  
Electrocatalytic Activity ◽  
Meldola's Blue ◽  
Meldola’S Blue

scholarly journals Detection of NADH and ethanol at a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite film

2012 ◽  
Vol 10 (1) ◽  
pp. 224-231 ◽  
Author(s):  
Jolanta Kochana ◽  
Juliusz Adamski
Keyword(s):  
Nanocomposite Film ◽  
Graphite Electrode ◽  
Limit Of Detection ◽  
Sol Gel ◽  
The Matrix ◽  
Titania Sol ◽  
Electrocatalytic Determination ◽  
Meldola's Blue ◽  
Meldola’S Blue

AbstractFor electrocatalytic determination of NADH, a graphite electrode modified with titania sol-gel/Meldola’s Blue/MWCNT/Nafion nanocomposite was proposed. The composition of the matrix film was optimised in terms of the content of carbon nanotubes and Nafion. Incorporation of a redox mediator, Meldola’s Blue, into the nanocomposite film enabled electrocatalytic determination of NADH at a low potential, −50 mV. For determination of ethanol, alcohol dehydrogenase (ADH) was immobilized into the matrix layer. Experimental conditions affecting the biosensor response were examined, including enzyme loading, temperature of measurement and pH of background electrolyte. Assessments of the analytical characteristics of the biosensor were performed with respect to sensitivity, limit of detection, operational stability, repeatability and reproducibility. The proposed biosensor showed electrocatalytic activity toward oxidation of ethanol with sensitivity of 2.24 µA L mmol−1, linear range from 0.05 to 1.1 mmol L−1, and limit of detection of 25 µmol L−1. The apparent Michaelis-Menten constant was 1.24 mmol L−1, indicating a high biological affinity of ADH/titania sol-gel/Meldola’s Blue/MWCNT/Nafion electrode for ethanol. The developed biosensor was tested in determinations of ethanol content in alcoholic beverages.

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