Lengthening the Lifetime of Common Emissive Probes to Microseconds by a Jigsaw‐Like Construction of NIR‐Responsive Nanohybrids

2020 ◽  
Vol 8 (8) ◽  
pp. 1902030 ◽  
Author(s):  
Nestor Estebanez ◽  
Juan Ferrera‐González ◽  
Ian Alberto Cortez‐Cevallos ◽  
María González‐Béjar ◽  
Julia Pérez‐Prieto
Keyword(s):  
Emissive Probes

Note: On the measurement of plasma potential fluctuations using emissive probes

2014 ◽  
Vol 85 (5) ◽  
pp. 056102 ◽  
Author(s):  
G. Bousselin ◽  
N. Lemoine ◽  
J. Cavalier ◽  
S. Heuraux ◽  
G. Bonhomme
Keyword(s):  
Plasma Potential ◽  
Potential Fluctuations ◽  
Emissive Probes

Cellular membrane-anchored fluorescent probe with aggregation-induced emission characteristics for selective detection of Cu2+ ions

2017 ◽  
Vol 196 ◽  
pp. 377-393 ◽  
Author(s):  
Danni Liu ◽  
Shenglu Ji ◽  
Heran Li ◽  
Liang Hong ◽  
Deling Kong ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Cell Membrane ◽  
Water Solubility ◽  
Live Cells ◽  
Probe Design ◽  
Specific Cell ◽  
Selective Detection ◽  
High Concentration ◽  
Aggregation Induced Emission ◽  
Emissive Probes

The exploration of advanced fluorescent probes that can detect divalent copper (Cu2+) in aqueous environments and even in live organisms is particularly valuable for understanding the occurrence and development of Cu2+-related diseases. In this work, we report the design and synthesis of an aggregation-induced emission luminogen (AIEgen)-based probe (TPE-Py-EEGTIGYG) by integrating an AIEgen, TPE-Py, with a peptide, EEGTIGYG, which can selectively detect Cu2+ in both aqueous solution and live cells. Peptide EEGTIGYG has dual functionality in the probe design, namely improving water solubility and providing specific cell membrane-binding ability. TPE-Py-EEGTIGYG can self-assemble into nanoaggregates at high concentration in aqueous solution (e.g., 25 μM), which possess large fluorescence output due to the restriction of intramolecular rotation of the phenyl rings on TPE-Py. The fluorescence of the TPE-Py-EEGTIGYG nanoaggregates can be significantly quenched by Cu2+ but not by other metal ions, achieving the selective detection of Cu2+ in aqueous media. Furthermore, TPE-Py-EEGTIGYG can exist as a molecular species and is very weakly fluorescent in dilute aqueous solution (e.g., 5 μM), but can however largely switch on its fluorescence upon specifically anchoring onto the cell membrane. The emissive probes on the cell membrane can be used for the detection of Cu2+ ions that move in and out of cells with a fluorescence “turn-off” mode.

Comparison of Langmuir and emissive probes as diagnostics for turbulence studies in the low-temperature plasma of the torsatron TJ-K

2005 ◽  
Vol 47 (4) ◽  
pp. 569-579 ◽  
Author(s):  
N Mahdizadeh ◽  
F Greiner ◽  
M Ramisch ◽  
U Stroth ◽  
W Guttenfelder ◽  
...  
Keyword(s):  
Low Temperature ◽  
Low Temperature Plasma ◽  
Temperature Plasma ◽  
Emissive Probes

scholarly journals Spectroscopically Well-Characterized RGD Optical Probe as a Prerequisite for Lifetime-Gated Tumor Imaging

2011 ◽  
Vol 10 (6) ◽  
pp. 7290.2011.00018 ◽  
Author(s):  
Julia Eva Mathejczyk ◽  
Jutta Pauli ◽  
Christian Dullin ◽  
Joanna Napp ◽  
Lutz-F. Tietze ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Nude Mice ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Specific Binding ◽  
Optical Probe ◽  
Detection Sensitivity ◽  
Rgd Peptides ◽  
Emissive Probes

Labeling of RGD peptides with near-infrared fluorophores yields optical probes for noninvasive imaging of tumors overexpressing αvβ3 integrins. An important prerequisite for optimum detection sensitivity in vivo is strongly absorbing and highly emissive probes with a known fluorescence lifetime. The RGD-Cy5.5 optical probe was derived by coupling Cy5.5 to a cyclic arginine–glycine–aspartic acid–d-phenylalanine–lysine (RGDfK) peptide via an aminohexanoic acid spacer. Spectroscopic properties of the probe were studied in different matrices in comparison to Cy5.5. For in vivo imaging, human glioblastoma cells were subcutaneously implanted into nude mice, and in vivo fluorescence intensity and lifetime were measured. The fluorescence quantum yield and lifetime of Cy5.5 were found to be barely affected on RGD conjugation but dramatically changed in the presence of proteins. By time domain fluorescence imaging, we demonstrated specific binding of RGD-Cy5.5 to glioblastoma xenografts in nude mice. Discrimination of unspecific fluorescence by lifetime-gated analysis further enhanced the detection sensitivity of RGD-Cy5.5-derived signals. We characterized RGD-Cy5.5 as a strongly emissive and stable probe adequate for selective targeting of αvβ3 integrins. The specificity and thus the overall detection sensitivity in vivo were optimized with lifetime gating, based on the previous determination of the probes fluorescence lifetime under application-relevant conditions.

scholarly journals Recommended Practice for Use of Emissive Probes in Electric Propulsion Testing

2017 ◽  
Vol 33 (3) ◽  
pp. 614-637 ◽  
Author(s):  
J. P. Sheehan ◽  
Yevgeny Raitses ◽  
Noah Hershkowitz ◽  
Michael McDonald
Keyword(s):  
Electric Propulsion ◽  
Emissive Probes

Turbulence and transport measurements with cold and emissive probes in ISTTOK

2008 ◽  
Vol 50 (5) ◽  
pp. 055004 ◽  
Author(s):  
R Schrittwieser ◽  
C Ionita ◽  
P Balan ◽  
C Silva ◽  
H Figueiredo ◽  
...  
Keyword(s):  
Transport Measurements ◽  
Emissive Probes

The Use of Emissive Probes in Laboratory and Tokamak Plasmas

2011 ◽  
Vol 51 (2-3) ◽  
pp. 264-270 ◽  
Author(s):  
C. Ionita ◽  
J. Grünwald ◽  
Ch. Maszl ◽  
R. Stärz ◽  
M. Čerček ◽  
...  
Keyword(s):  
Tokamak Plasmas ◽  
Emissive Probes
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