scholarly journals Surface Plasmon Resonance Microscopy: Correlating Surface Plasmon Resonance Microscopy of Living and Fixated Cells with Electron Microscopy Allows for Investigation of Potential Preparation Artifacts (Adv. Mater. Interfaces 6/2020)

2020 ◽  
Vol 7 (6) ◽  
pp. 2070026 ◽  
Author(s):  
Eva Kreysing ◽  
Silke Seyock ◽  
Hossein Hassani ◽  
Elke Brauweiler‐Reuters ◽  
Elmar Neumann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Microscopy ◽  
Potential Preparation

scholarly journals Correlating surface plasmon resonance microscopy of living and fixated cells with electron microscopy allows for investigation of potential preparation artifacts

2019 ◽  
Author(s):  
Eva Kreysing ◽  
Silke Seyock ◽  
Hossein Hassani ◽  
Elke Brauweiler-Reuters ◽  
Elmar Neumann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Surface Plasmon Resonance ◽  
Sample Preparation ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Chemical Fixation ◽  
Substrate Interface ◽  
Cell Substrate ◽  
Surface Plasmon Resonance Microscopy ◽  
The Impact

AbstractThe investigation of the cell-substrate interface is of great importance for a broad spectrum of areas such as biomedical engineering, brain-chip interfacing and fundamental research. Due to its unique resolution and the prevalence of instruments, electron microscopy (EM) is used as one of the standard techniques for the analysis of the cell-substrate interface. However, possible artifacts that might be introduced by the required sample preparation have been the subject of speculation for decades. Due to recent advances in Surface plasmon resonance microscopy (SPRM), the technique now offers a label-free alternative for the interface characterization with nanometer resolution in axial direction. In contrast to EM, SPRM studies do not require fixation and can therefore be performed on living cells. Here, we present a workflow that allows us to quantify the impact of chemical fixation on the cell-substrate interface. These measurements confirmed that chemical fixation preserved the average cell-substrate distances in the majority of studied cells. Furthermore, we were able to correlate the SPRM measurements with EM images of the cell-substrate interface of the exact same cells allowing us to identify regions with good agreement between the two methods and reveal artifacts introduced during further sample preparation.

scholarly journals Palladium nanoparticles anchored to anatase TiO2 for enhanced surface plasmon resonance-stimulated, visible-light-driven photocatalytic activity

2015 ◽  
Vol 6 ◽  
pp. 428-437 ◽  
Author(s):  
Kah Hon Leong ◽  
Hong Ye Chu ◽  
Shaliza Ibrahim ◽  
Pichiah Saravanan
Keyword(s):  
Electron Microscopy ◽  
Photocatalytic Activity ◽  
Surface Plasmon Resonance ◽  
Visible Light ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Palladium Nanoparticles ◽  
Noble Metals ◽  
Visible Region ◽  
Bet Surface Area

Freely assembled palladium nanoparticles (Pd NPs) on titania (TiO2) nano photocatalysts were successfully synthesized through a photodeposition method using natural sunlight. This synthesized heterogeneous photocatalyst (Pd/TiO2) was characterized through field emission scanning electron microscopy (FESEM), high resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), BET surface area, UV–vis diffuse reflectance spectra (UV-DRS), Raman and photoluminescence (PL) analyses. The simple and smart synthesis anchored well the deposition with controlled Pd NPs size ranging between 17 and 29 nm onto the surface of TiO2. Thus, it gives the characteristic for Pd NPs to absorb light in the visible region obtained through localized surface plasmon resonance (LSPRs). Apparently, the photocatalytic activity of the prepared photocatalysts was evaluated by degrading the endocrine disrupting compound (EDC) amoxicillin (AMX) excited under an artificial visible light source. In the preliminary run, almost complete degradation (97.5%) was achieved in 5 h with 0.5 wt % Pd loading and the degradation followed pseudo-first-order kinetics. The reusability trend proved the photostability of the prepared photocatalysts. Hence, the study provides a new insight about the modification of TiO2 with noble metals in order to enhance the absorption in the visible-light region for superior photocatalytic performance.

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