Development of a Novel Orthogonal Double Gradient for High-Throughput Screening of Mesenchymal Stem Cells-Materials Interaction

2018 ◽  
Vol 5 (18) ◽  
pp. 1800504 ◽  
Author(s):  
Qihui Zhou ◽  
Lu Ge ◽  
Carlos F. Guimarães ◽  
Philipp T. Kühn ◽  
Liangliang Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
High Throughput Screening

scholarly journals High content and high throughput screening to assess the angiogenic and neurogenic actions of mesenchymal stem cells in vitro

2015 ◽  
Vol 333 (1) ◽  
pp. 93-104 ◽  
Author(s):  
Jennifer J. Bara ◽  
Sarah Turner ◽  
Sally Roberts ◽  
Gareth Griffiths ◽  
Rod Benson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
High Throughput Screening

scholarly journals Non-invasive and high-throughput interrogation of exon-specific isoform expression

2021 ◽  
Author(s):  
Dong-Jiunn Jeffery Truong ◽  
Teeradon Phlairaharn ◽  
Bianca Eßwein ◽  
Christoph Gruber ◽  
Deniz Tümen ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Throughput ◽  
High Throughput Screening ◽  
Embryonic Stem ◽  
High Sensitivity ◽  
Therapeutic Interventions ◽  
Dominant Factor ◽  
Reporter System ◽  
Isoform Expression ◽  
Induced Pluripotent

AbstractExpression of exon-specific isoforms from alternatively spliced mRNA is a fundamental mechanism that substantially expands the proteome of a cell. However, conventional methods to assess alternative splicing are either consumptive and work-intensive or do not quantify isoform expression longitudinally at the protein level. Here, we therefore developed an exon-specific isoform expression reporter system (EXSISERS), which non-invasively reports the translation of exon-containing isoforms of endogenous genes by scarlessly excising reporter proteins from the nascent polypeptide chain through highly efficient, intein-mediated protein splicing. We applied EXSISERS to quantify the inclusion of the disease-associated exon 10 in microtubule-associated protein tau (MAPT) in patient-derived induced pluripotent stem cells and screened Cas13-based RNA-targeting effectors for isoform specificity. We also coupled cell survival to the inclusion of exon 18b of FOXP1, which is involved in maintaining pluripotency of embryonic stem cells, and confirmed that MBNL1 is a dominant factor for exon 18b exclusion. EXSISERS enables non-disruptive and multimodal monitoring of exon-specific isoform expression with high sensitivity and cellular resolution, and empowers high-throughput screening of exon-specific therapeutic interventions.

scholarly journals High Throughput Transcriptome Profiling of Lithium Stimulated Human Mesenchymal Stem Cells Reveals Priming towards Osteoblastic Lineage

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e55769 ◽  
Author(s):  
Neeraj Kumar Satija ◽  
Deepa Sharma ◽  
Farhat Afrin ◽  
Rajendra P. Tripathi ◽  
Gurudutta Gangenahalli
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
Human Mesenchymal Stem Cells ◽  
Transcriptome Profiling ◽  
Osteoblastic Lineage

scholarly journals High-Throughput Screening Enhances Kidney Organoid Differentiation from Human Pluripotent Stem Cells and Enables Automated Multidimensional Phenotyping

2018 ◽  
Vol 22 (6) ◽  
pp. 929-940.e4 ◽  
Author(s):  
Stefan M. Czerniecki ◽  
Nelly M. Cruz ◽  
Jennifer L. Harder ◽  
Rajasree Menon ◽  
James Annis ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Throughput ◽  
Pluripotent Stem Cells ◽  
High Throughput Screening ◽  
Human Pluripotent Stem Cells

scholarly journals High-Throughput Screening Assay for the Identification of Compounds Regulating Self-Renewal and Differentiation in Human Embryonic Stem Cells

2008 ◽  
Vol 2 (6) ◽  
pp. 602-612 ◽  
Author(s):  
Sabrina C. Desbordes ◽  
Dimitris G. Placantonakis ◽  
Anthony Ciro ◽  
Nicholas D. Socci ◽  
Gabsang Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
High Throughput ◽  
Human Embryonic Stem Cells ◽  
High Throughput Screening ◽  
Embryonic Stem ◽  
Screening Assay ◽  
Self Renewal ◽  
High Throughput Screening Assay
Sign in / Sign up

Export Citation Format

Share Document