scholarly journals 4D‐Printed Transformable Tube Array for High‐Throughput 3D Cell Culture and Histology

2020 ◽  
Vol 32 (40) ◽  
pp. 2004285 ◽  
Author(s):  
Chen Yang ◽  
Jeffrey Luo ◽  
Marianne Polunas ◽  
Nikola Bosnjak ◽  
Sy‐Tsong Dean Chueng ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
3D Cell Culture

Patterned superhydrophobic surfaces to process and characterize biomaterials and 3D cell culture

2018 ◽  
Vol 5 (3) ◽  
pp. 379-393 ◽  
Author(s):  
A. I. Neto ◽  
P. A. Levkin ◽  
J. F. Mano
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
High Throughput Screening ◽  
3D Cell Culture ◽  
Superhydrophobic Surfaces ◽  
Technological Breakthrough ◽  
Large Numbers

Microarrays are a technological breakthrough for high-throughput screening of large numbers of assays.

scholarly journals High-Throughput Assessment of Mechanistic Toxicity of Chemicals in Miniaturized 3D Cell Culture

2018 ◽  
Vol 79 (1) ◽  
pp. e66
Author(s):  
Pranav Joshi ◽  
Soo-Yeon Kang ◽  
Akshata Datar ◽  
Moo-Yeal Lee
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
3D Cell Culture

scholarly journals Aspiration-mediated hydrogel micropatterning using rail-based open microfluidic devices for high-throughput 3D cell culture

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Dohyun Park ◽  
Jungseub Lee ◽  
Younggyun Lee ◽  
Kyungmin Son ◽  
Jin Woo Choi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Capillary Pressure ◽  
Microfluidic Device ◽  
High Throughput ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
Cell Types ◽  
Human Organ ◽  
Patterning Technique ◽  
Experimental Yield

AbstractMicrofluidics offers promising methods for aligning cells in physiologically relevant configurations to recapitulate human organ functionality. Specifically, microstructures within microfluidic devices facilitate 3D cell culture by guiding hydrogel precursors containing cells. Conventional approaches utilize capillary forces of hydrogel precursors to guide fluid flow into desired areas of high wettability. These methods, however, require complicated fabrication processes and subtle loading protocols, thus limiting device throughput and experimental yield. Here, we present a swift and robust hydrogel patterning technique for 3D cell culture, where preloaded hydrogel solution in a microfluidic device is aspirated while only leaving a portion of the solution in desired channels. The device is designed such that differing critical capillary pressure conditions are established over the interfaces of the loaded hydrogel solution, which leads to controlled removal of the solution during aspiration. A proposed theoretical model of capillary pressure conditions provides physical insights to inform generalized design rules for device structures. We demonstrate formation of multiple, discontinuous hollow channels with a single aspiration. Then we test vasculogenic capacity of various cell types using a microfluidic device obtained by our technique to illustrate its capabilities as a viable micro-manufacturing scheme for high-throughput cellular co-culture.

Remote-Controlled 3D Porous Magnetic Interface toward High-Throughput Dynamic 3D Cell Culture

2021 ◽  
Vol 7 (9) ◽  
pp. 4535-4544
Author(s):  
Bryce J. Stottlemire ◽  
Aparna R. Chakravarti ◽  
Jonathan W. Whitlow ◽  
Cory J. Berkland ◽  
Mei He
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
3D Cell Culture

Hydrogel-incorporating unit in a well: 3D cell culture for high-throughput analysis

Lab on a Chip ◽  
2018 ◽  
Vol 18 (17) ◽  
pp. 2604-2613 ◽  
Author(s):  
Yeong Jun Yu ◽  
Young Hye Kim ◽  
Kyuhwan Na ◽  
Seo Yun Min ◽  
Ok Kyung Hwang ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Culture System ◽  
3D Cell Culture ◽  
High Content Screening ◽  
Cell Culture System ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Biochemical Assays

A microchannel-free, 3D cell culture system has a hydrogel-incorporating unit integrated with a multi-well plate. This plate provides better reproducibility in a variety of quantitative biochemical assays and high content-screening (HCS).

scholarly journals Bioluminescence Imaging of Spheroids for High-throughput Longitudinal Studies on 3D Cell Culture Models

2017 ◽  
Vol 93 (2) ◽  
pp. 531-535 ◽  
Author(s):  
Luca Cevenini ◽  
Maria M. Calabretta ◽  
Antonia Lopreside ◽  
Bruce R. Branchini ◽  
Tara L. Southworth ◽  
...  
Keyword(s):  
Cell Culture ◽  
Longitudinal Studies ◽  
High Throughput ◽  
Bioluminescence Imaging ◽  
3D Cell Culture ◽  
Culture Models ◽  
Cell Culture Models

Estimation of bisphenol A—Human toxicity by 3D cell culture arrays, high throughput alternatives to animal tests

2016 ◽  
Vol 259 ◽  
pp. 87-94 ◽  
Author(s):  
Dong Woo Lee ◽  
Woo-Yeon Oh ◽  
Sang Hyun Yi ◽  
Bosung Ku ◽  
Moo-Yeal Lee ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bisphenol A ◽  
High Throughput ◽  
3D Cell Culture ◽  
Human Toxicity ◽  
Animal Tests
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