scholarly journals A Cyclometalated Iridium (III) Complex as a Microtubule Probe for Correlative Super‐Resolution Fluorescence and Electron Microscopy

2020 ◽  
Vol 32 (39) ◽  
pp. 2003901
Author(s):  
Xiaohe Tian ◽  
Cesare De Pace ◽  
Lorena Ruiz‐Perez ◽  
Bo Chen ◽  
Rina Su ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Super Resolution ◽  
Fluorescence And Electron Microscopy

scholarly journals Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution

2014 ◽  
Vol 127 (20) ◽  
pp. 4351-4355 ◽  
Author(s):  
Anna Löschberger ◽  
Christian Franke ◽  
Georg Krohne ◽  
Sebastian van de Linde ◽  
Markus Sauer
Keyword(s):  
Electron Microscopy ◽  
Nuclear Pore Complex ◽  
Super Resolution ◽  
Nuclear Pore ◽  
Fluorescence And Electron Microscopy

scholarly journals Correlative super-resolution fluorescence and electron microscopy using conventional fluorescent proteins in vacuo

2017 ◽  
Vol 199 (2) ◽  
pp. 120-131 ◽  
Author(s):  
Christopher J. Peddie ◽  
Marie-Charlotte Domart ◽  
Xenia Snetkov ◽  
Peter O'Toole ◽  
Banafshe Larijani ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Fluorescence And Electron Microscopy

scholarly journals Correlated super-resolution fluorescence and electron microscopy reveals the catalytically active nanorods within individual H-ZSM-22 zeolite particles

2019 ◽  
Vol 9 (17) ◽  
pp. 4645-4650 ◽  
Author(s):  
Arunasish Layek ◽  
Jordi Van Loon ◽  
Maarten B. J. Roeffaers ◽  
Alexey V. Kubarev
Keyword(s):  
Electron Microscopy ◽  
Catalytic Activity ◽  
Growth Mechanism ◽  
Super Resolution ◽  
Fluorescence And Electron Microscopy ◽  
Catalytically Active ◽  
Structural Imperfections

Correlative fluorescence and electron microscopy finds heterogeneity in the catalytic activity of H-ZSM-22 zeolite, related to the growth mechanism and structural imperfections.

scholarly journals Cryogenic Super-Resolution Fluorescence and Electron Microscopy Correlated at the Nanoscale

2021 ◽  
Vol 72 (1) ◽  
Author(s):  
Peter D. Dahlberg ◽  
W.E. Moerner
Keyword(s):  
Electron Microscopy ◽  
Single Molecule ◽  
Electron Tomography ◽  
Light And Electron Microscopy ◽  
Super Resolution ◽  
Nanometer Scale ◽  
Annual Review ◽  
Publication Date ◽  
Fluorescence And Electron Microscopy ◽  
Cellular Context

We review the emerging method of super-resolved cryogenic correlative light and electron microscopy (srCryoCLEM). Super-resolution (SR) fluorescence microscopy and cryogenic electron tomography (CET) are both powerful techniques for observing subcellular organization, but each approach has unique limitations. The combination of the two brings the single-molecule sensitivity and specificity of SR to the detailed cellular context and molecular scale resolution of CET. The resulting correlative data is more informative than the sum of its parts. The correlative images can be used to pinpoint the positions of fluorescently labeled proteins in the high-resolution context of CET with nanometer-scale precision and/or to identify proteins in electron-dense structures. The execution of srCryoCLEM is challenging and the approach is best described as a method that is still in its infancy with numerous technical challenges. In this review, we describe state-of-the-art srCryoCLEM experiments, discuss the most pressing challenges, and give a brief outlook on future applications. Expected final online publication date for the Annual Review of Physical Chemistry, Volume 72 is April 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

scholarly journals Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells

2019 ◽  
Author(s):  
David P. Hoffman ◽  
Gleb Shtengel ◽  
C. Shan Xu ◽  
Kirby R. Campbell ◽  
Melanie Freeman ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
Super Resolution ◽  
Field Of View ◽  
Whole Cells ◽  
Workflow Optimization ◽  
Fluorescence And Electron Microscopy ◽  
Block Face

AbstractLiving cells function through the spatial compartmentalization of thousands of distinct proteins serving a multitude of diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) has emerged as a pathway to directly view nanoscale protein relationships to the underlying global ultrastructure, but has traditionally suffered from tradeoffs of structure preservation, fluorescence retention, resolution, and field of view. We developed a platform for three-dimensional correlative cryogenic SR and focused ion beam milled block-face EM across entire vitreously frozen cells that addresses these issues by preserving native ultrastructure and enabling independent SR and EM workflow optimization. Application to a variety of biological systems revealed a number of unexpected protein-ultrastructure relationships and underscored the value of a comprehensive multimodal view of ultrastructural variability across whole cells.

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