Ultrahigh‐Sensitivity Sandwiched Plasmon Ruler for Label‐Free Clinical Diagnosis

2019 ◽  
Vol 32 (2) ◽  
pp. 1905927 ◽  
Author(s):  
Jingjie Nan ◽  
Shoujun Zhu ◽  
Shunsheng Ye ◽  
Weihong Sun ◽  
Ying Yue ◽  
...  
Keyword(s):  
Clinical Diagnosis ◽  
Label Free ◽  
Ultrahigh Sensitivity ◽  
Plasmon Ruler

scholarly journals Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 448
Author(s):  
Vien Thi Tran ◽  
Heongkyu Ju
Keyword(s):  
Surface Plasmon ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Limit Of Detection ◽  
Delayed Diagnosis ◽  
Cardiac Troponin I ◽  
Quantitative Detection ◽  
Fluorescence Signal ◽  
Label Free ◽  
Ultrahigh Sensitivity

This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL−1 (atto-gram mL−1) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity.

Mueller Matrix Polarimetry: A Label-Free, Quantitative Optical Method for Clinical Diagnosis

2020 ◽  
Vol 47 (2) ◽  
pp. 0207001
Author(s):  
沈元星 Shen Yuanxing ◽  
姚悦 Yao Yue ◽  
何宏辉 He Honghui ◽  
刘少雄 Liu Shaoxiong ◽  
马辉 Ma Hui
Keyword(s):  
Clinical Diagnosis ◽  
Optical Method ◽  
Mueller Matrix ◽  
Label Free

Target-triggered cascade recycling amplification for label-free detection of microRNA and molecular logic operations

2016 ◽  
Vol 52 (2) ◽  
pp. 402-405 ◽  
Author(s):  
Sai Bi ◽  
Jiayan Ye ◽  
Ying Dong ◽  
Haoting Li ◽  
Wei Cao
Keyword(s):  
Logic Gates ◽  
Logic Circuits ◽  
Label Free ◽  
Chemiluminescence Detection ◽  
Molecular Logic ◽  
Label Free Detection ◽  
Molecular Scale ◽  
Ultrahigh Sensitivity ◽  
Recycling Amplification ◽  
Logic Operations

A cascade recycling amplification (CRA) that implements cascade logic circuits with feedback amplification function is developed for label-free chemiluminescence detection of microRNA-122 with an ultrahigh sensitivity of 0.82 fM and excellent specificity, which is applied to construct a series of molecular-scale two-input logic gates by using microRNAs as inputs and CRA products as outputs.

scholarly journals Development of SPR Imaging-Impedance Sensor for Multi-Parametric Living Cell Analysis

Sensors ◽  
2019 ◽  
Vol 19 (9) ◽  
pp. 2067 ◽  
Author(s):  
Yuhki Yanase ◽  
Kyohei Yoshizaki ◽  
Kaiken Kimura ◽  
Tomoko Kawaguchi ◽  
Michihiro Hide ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Diagnosis ◽  
Living Cell ◽  
Living Cells ◽  
Sensor Chip ◽  
Type I ◽  
Label Free ◽  
Cell Functions ◽  
Spr Imaging ◽  
Impedance Sensor

Label-free evaluation and monitoring of living cell conditions or functions by means of chemical and/or physical sensors in a real-time manner are increasingly desired in the field of basic research of cells and clinical diagnosis. In order to perform multi-parametric analysis of living cells on a chip, we here developed a surface plasmon resonance (SPR) imaging (SPRI)-impedance sensor that can detect both refractive index (RI) and impedance changes on a sensor chip with comb-shaped electrodes. We then investigated the potential of the sensor for label-free and real-time analysis of living cell reactions in response to stimuli. We cultured rat basophilic leukemia (RBL)-2H3 cells on the sensor chip, which was a glass slide coated with comb-shaped electrodes, and detected activation of RBL-2H3 cells, such as degranulation and morphological changes, in response to a dinitro-phenol-conjugated human serum albumin (DNP-HSA) antigen. Moreover, impedance analysis revealed that the changes of impedance derived from RBL-2H3 cell activation appeared in the range of 1 kHz–1 MHz. Furthermore, we monitored living cell-derived RI and impedance changes simultaneously on a sensor chip using the SPRI-impedance sensor. Thus, we developed a new technique to monitor both impedance and RI derived from living cells by using a comb-shaped electrode sensor chip. This technique may enable us to clarify complex living cell functions which affect the RI and impedance and apply this to medical applications, such as accurate clinical diagnosis of type I allergy.

scholarly journals Raman Spectroscopy and Microscopy Applications in Cardiovascular Diseases: From Molecules to Organs

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 107 ◽  
Author(s):  
Ardalan Chaichi ◽  
Alisha Prasad ◽  
Manas Gartia
Keyword(s):  
Computed Tomography ◽  
Raman Spectroscopy ◽  
Cardiovascular Diseases ◽  
Clinical Diagnosis ◽  
Vibrational Spectroscopy ◽  
Single Photon ◽  
Photon Emission ◽  
Biological Tissues ◽  
Emission Computed Tomography ◽  
Label Free

Noninvasive and label-free vibrational spectroscopy and microscopy methods have shown great potential for clinical diagnosis applications. Raman spectroscopy is based on inelastic light scattering due to rotational and vibrational modes of molecular bonds. It has been shown that Raman spectra provide chemical signatures of changes in biological tissues in different diseases, and this technique can be employed in label-free monitoring and clinical diagnosis of several diseases, including cardiovascular studies. However, there are very few literature reviews available to summarize the state of art and future applications of Raman spectroscopy in cardiovascular diseases, particularly cardiac hypertrophy. In addition to conventional clinical approaches such as electrocardiography (ECG), echocardiogram (cardiac ultrasound), positron emission tomography (PET), cardiac computed tomography (CT), and single photon emission computed tomography (SPECT), applications of vibrational spectroscopy and microscopy will provide invaluable information useful for the prevention, diagnosis, and treatment of cardiovascular diseases. Various in vivo and ex vivo investigations can potentially be performed using Raman imaging to study and distinguish pathological and physiological cardiac hypertrophies and understand the mechanisms of other cardiac diseases. Here, we have reviewed the recent literature on Raman spectroscopy to study cardiovascular diseases covering investigations on the molecular, cellular, tissue, and organ level.

Label-Free Nanoplasmonic Biosensing of Cancer Biomarkers for Clinical Diagnosis

2019 ◽  
pp. 115-140 ◽  
Author(s):  
Alejandro Portela ◽  
Enelia C. Peláez ◽  
Olalla Calvo-Lozano ◽  
Mari C. Estévez ◽  
Laura M. Lechuga
Keyword(s):  
Clinical Diagnosis ◽  
Cancer Biomarkers ◽  
Label Free
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