scholarly journals Polymer Nanoparticles: Synthesis and Assembly of Click-Nucleic-Acid-Containing PEG-PLGA Nanoparticles for DNA Delivery (Adv. Mater. 24/2017)

2017 ◽  
Vol 29 (24) ◽  
Author(s):  
Albert Harguindey ◽  
Dylan W. Domaille ◽  
Benjamin D. Fairbanks ◽  
Justine Wagner ◽  
Christopher N. Bowman ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Delivery ◽  
Plga Nanoparticles ◽  
Polymer Nanoparticles ◽  
Nanoparticles Synthesis

scholarly journals Synthesis and Assembly of Click-Nucleic-Acid-Containing PEG-PLGA Nanoparticles for DNA Delivery

2017 ◽  
Vol 29 (24) ◽  
pp. 1700743 ◽  
Author(s):  
Albert Harguindey ◽  
Dylan W. Domaille ◽  
Benjamin D. Fairbanks ◽  
Justine Wagner ◽  
Christopher N. Bowman ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Delivery ◽  
Plga Nanoparticles

Fluorescent Heterotelechelic Single-Chain Polymer Nanoparticles: Synthesis, Spectroscopy, and Cellular Imaging

2019 ◽  
Vol 2 (2) ◽  
pp. 898-909 ◽  
Author(s):  
Daniel N. F. Bajj ◽  
Michael V. Tran ◽  
Hsin-Yun Tsai ◽  
Hyungki Kim ◽  
Nathan R. Paisley ◽  
...  
Keyword(s):  
Cellular Imaging ◽  
Polymer Nanoparticles ◽  
Single Chain ◽  
Chain Polymer ◽  
Nanoparticles Synthesis

scholarly journals Non-Viral Delivery of RNA Gene Therapy to the Central Nervous System

Pharmaceutics ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 165
Author(s):  
Ellen S. Hauck ◽  
James G. Hecker
Keyword(s):  
Gene Therapy ◽  
Central Nervous System ◽  
Nervous System ◽  
Nucleic Acid ◽  
Gene Delivery ◽  
Dna Delivery ◽  
Clinical Applications ◽  
Cationic Lipids ◽  
Viral Dna ◽  
The Central Nervous System

Appropriate gene delivery systems are essential for successful gene therapy in clinical medicine. Lipid-mediated nucleic acid delivery is an alternative to viral vector-mediated gene delivery and has the following advantages. Lipid-mediated delivery of DNA or mRNA is usually more rapid than viral-mediated delivery, offers a larger payload, and has a nearly zero risk of incorporation. Lipid-mediated delivery of DNA or RNA is therefore preferable to viral DNA delivery in those clinical applications that do not require long-term expression for chronic conditions. Delivery of RNA may be preferable to non-viral DNA delivery in some clinical applications, since transit across the nuclear membrane is not necessary, and onset of expression with RNA is therefore even faster than with DNA, although both are faster than most viral vectors. Delivery of RNA to target organ(s) has previously been challenging due to RNA’s rapid degradation in biological systems, but cationic lipids complexed with RNA, as well as lipid nanoparticles (LNPs), have allowed for delivery and expression of the complexed RNA both in vitro and in vivo. This review will focus on the non-viral lipid-mediated delivery of RNAs, including mRNA, siRNA, shRNA, and microRNA, to the central nervous system (CNS), an organ with at least two unique challenges. The CNS contains a large number of slowly dividing or non-dividing cell types and is protected by the blood brain barrier (BBB). In non-dividing cells, RNA-lipid complexes demonstrated increased transfection efficiency relative to DNA transfection. The efficiency, timing of the onset, and duration of expression after transfection may determine which nucleic acid is best for which proposed therapy. Expression can be seen as soon as 1 h after RNA delivery, but duration of expression has been limited to 5–7 h. In contrast, transfection with a DNA lipoplex demonstrates protein expression within 5 h and lasts as long as several weeks after transfection.

Active Polymer Nanoparticles: Delivery of Antibiotics

2007 ◽  
Vol 1019 ◽  
Author(s):  
Monica Rabinovich ◽  
Shankari N. Somayaji ◽  
Rajeev Raghavan Pillai ◽  
Michael C. Hudson ◽  
J. Kent Ellington ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Drug Loading ◽  
Plga Nanoparticles ◽  
Polymer Nanoparticles ◽  
Copolymer Composition ◽  
Zns Nanoparticles ◽  
Primary Mouse ◽  
Evaporation Technique ◽  
Uv Visible ◽  
Scanning Electron

AbstractAntibiotic-encapsulated PLA and PLGA nanoparticles were prepared by the single emulsion-solvent evaporation technique. Different PLA and PLGA systems were prepared, varying the copolymer composition and the amount of the surfactant polyvinyl alcohol. Characterization and drug loading studies were performed by UV-Visible spectrophotometry, dynamic light scattering, and scanning electron microscopy (SEM).Simultaneously, in order to model the diffusion of the nanoparticles within the osteoblast, QDs such as functionalized InGaP/ZnS and polymer encapsulated InGaP/ZnS nanoparticles were added to confluent cultures of primary mouse osteoblasts. Following PreFer fixation, cultures were examined via confocal microscopy. QDs were clearly visible within osteoblasts.

Development of Oral Formulation Technology for Nucleic Acid Drug by Using PLGA Nanoparticles as DDS Carriers

2013 ◽  
Vol 50 (7) ◽  
pp. 513-518 ◽  
Author(s):  
Yusuke Tsukada ◽  
Hiroyuki Tsujimoto ◽  
Hajime Watanabe ◽  
Takayuki Sugimoto ◽  
Osami Nakano ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Oral Formulation ◽  
Plga Nanoparticles ◽  
Nucleic Acid Drug

scholarly journals Click Nucleic Acid Mediated Loading of Prodrug Activating Enzymes in PEG–PLGA Nanoparticles for Combination Chemotherapy

2019 ◽  
Vol 20 (4) ◽  
pp. 1683-1690 ◽  
Author(s):  
Albert Harguindey ◽  
Shambojit Roy ◽  
Alexander W. Harris ◽  
Benjamin D. Fairbanks ◽  
Andrew P. Goodwin ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Combination Chemotherapy ◽  
Plga Nanoparticles

scholarly journals Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

Polymers ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 341
Author(s):  
Veronika Subjakova ◽  
Veronika Oravczova ◽  
Tibor Hianik
Keyword(s):  
Nucleic Acid ◽  
Targeted Delivery ◽  
Controlled Drug Release ◽  
Polymer Nanoparticles ◽  
Rna Aptamers ◽  
Special Focus ◽  
Cancer Markers ◽  
Nucleic Acid Aptamers ◽  
Targeted Transport ◽  
Or Gene

Polymer nanoparticles and nano/micromotors are novel nanostructures that are of increased interest especially in the diagnosis and therapy of cancer. These structures are modified by antibodies or nucleic acid aptamers and can recognize the cancer markers at the membrane of the cancer cells or in the intracellular side. They can serve as a cargo for targeted transport of drugs or nucleic acids in chemo- immuno- or gene therapy. The various mechanisms, such as enzyme, ultrasound, magnetic, electrical, or light, served as a driving force for nano/micromotors, allowing their transport into the cells. This review is focused on the recent achievements in the development of polymer nanoparticles and nano/micromotors modified by antibodies and nucleic acid aptamers. The methods of preparation of polymer nanoparticles, their structure and properties are provided together with those for synthesis and the application of nano/micromotors. The various mechanisms of the driving of nano/micromotors such as chemical, light, ultrasound, electric and magnetic fields are explained. The targeting drug delivery is based on the modification of nanostructures by receptors such as nucleic acid aptamers and antibodies. Special focus is therefore on the method of selection aptamers for recognition cancer markers as well as on the comparison of the properties of nucleic acid aptamers and antibodies. The methods of immobilization of aptamers at the nanoparticles and nano/micromotors are provided. Examples of applications of polymer nanoparticles and nano/micromotors in targeted delivery and in controlled drug release are presented. The future perspectives of biomimetic nanostructures in personalized nanomedicine are also discussed.

Synthesis and Characterization of Click Nucleic Acid Conjugated Polymeric Microparticles for DNA Delivery Applications

2021 ◽  
Vol 22 (3) ◽  
pp. 1127-1136
Author(s):  
Alex J. Anderson ◽  
Emerson Grey ◽  
Nicholas J. Bongiardina ◽  
Christopher N. Bowman ◽  
Stephanie J. Bryant
Keyword(s):  
Nucleic Acid ◽  
Dna Delivery ◽  
Synthesis And Characterization ◽  
Polymeric Microparticles
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